40 results about "Pseudomonas mendocina" patented technology

The invention belongs to the technical field of water environment biological treatment, in particular to a Pseudomonas mendocina strain CY004 for strongly degrade nitrite nitrogen, nitrate nitrogen and ammonia nitrogen, separation screening, safety measurement, a bacterial liquid preparation method and treatment application to domestic sewage and aquaculture sewage. The strain CY004 is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 209197. By morphology observation, physiology and biochemistry analysis and 16S rDNA homology analysis, the strain CY004 is authenticated to be Pseudomonas mendocina ymp. The strain can quickly degrade the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the water body, has no pathogenicity on animals of silver carp, sharksucker, shrimp, Eriocheir sinensis, white mouse and the like, and better degradation function on the nitrite and the ammonia nitrogen in the real sewage under normal temperature, degradation rate reaching 96.1 percent and 90 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the aquaculture sewage, degradation rate reaching 97.3 percent and 97.1 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the domestic sewage and better application prospect.

Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.

The invention discloses a mannuronic acid C-5 epimerase / alginate lyase coding gene, enzyme, preparation and application, and belongs to the field of genetic engineering. Specifically disclosed is an enzyme gene derived from Pseudomonas mendocina, which has two functions of epimerizing mannuronic acid C-5 and cracking alginate, and the preparation method and application of the enzyme, namely Using the technical method of genetic engineering, clone the gene of the enzyme into the expression vector of Escherichia coli to obtain the recombinant strain of Escherichia coli that can express the enzyme heterologously, and the enzyme prepared by the heterologous expression of the strain can efficiently transform β-D- Conversion of mannuronic acid (M) to α‑L‑guluronic acid (G) and cleavage of alginate to oligosaccharides. The bifunctional enzyme provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine and the like.