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Pseudomonas stutzeri CY003 for efficiently removing trite nitrogen, nitrate nitrogen and ammonia nitrogen from water and application thereof

A technology of Pseudomonas stutzeri and nitrite nitrogen, applied in the direction of bacteria, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of long reaction start-up time and slow growth rate, and reduce follow-up work The effect of large quantity, strong adaptability and great development potential

Inactive Publication Date: 2010-06-09
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

This has become a bottleneck restricting the research and application of traditional biological denitrification technology: on the one hand, nitrous bacteria are chemoautotrophic bacteria with a slow growth rate, resulting in a long reaction start-up time, and nitrous bacteria are sensitive to the composition, pH and temperature of sewage. Sensitive to changes (Mobarry et al, 1996)

Method used

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  • Pseudomonas stutzeri CY003 for efficiently removing trite nitrogen, nitrate nitrogen and ammonia nitrogen from water and application thereof
  • Pseudomonas stutzeri CY003 for efficiently removing trite nitrogen, nitrate nitrogen and ammonia nitrogen from water and application thereof
  • Pseudomonas stutzeri CY003 for efficiently removing trite nitrogen, nitrate nitrogen and ammonia nitrogen from water and application thereof

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Experimental program
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Effect test

Embodiment 1

[0080] Embodiment 1, isolation, screening and identification of bacterial strains

[0081] 1. Isolation and screening of strains

[0082] 1. Prepare the following medium:

[0083] Denitrification medium: potassium nitrate 2.0g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 0.5g, potassium sodium tartrate 20g, distilled water 1L, pH 7.2 (adding 20g agar is a solid medium)

[0084] BTB screening medium: L-asparagine 1g, KNO 3 (or NaNO 2 )1g, KH 2 PO 4 1g, sodium succinate 8.5g, FeCl 2 .6H 2 O 0.05g, CaCl 2 .2H 2 O 0.2g, MgSO 4 .7H 2 O 0.07g, 1ml / L BTB [pH indicator bromothymol] (1%, soluble in ethanol), agar 20g, pH 7.2.

[0085] 2. Screening of aerobic denitrifying bacteria

[0086] ①. Microbial selective enrichment culture: put the water sample on hold and discard the supernatant, take the activated sludge and inoculate it in a test tube with a Duchenne tubule (denitrification medium), the inoculation amount is 5%, in an incubator at 28°C Af...

Embodiment 2

[0090] Example 2, Pseudomonas stutzeri CY003 to the determination of the removal ability of domestic sewage and aquaculture wastewater ammonia nitrogen

[0091] The domestic sewage used in the test was taken from the sewage outlet of Jingzhou Dongmen Moat, and the ammonia nitrogen concentration was measured to be 62.15mg / L, the nitrite concentration was 5.2mg / L, and pH6.0-6.8; the aquaculture wastewater was taken from the aquaculture sewage of the rice field eel breeding base of Yangtze University , the concentration of ammonia nitrogen is 5.72mg / L, the concentration of nitrite is 0.7mg / L, and the pH is 7.5-8.5. Set the concentration to 1 x 10 7 cfu / mL CY003 bacterial agent, put into the sewage according to the inoculum amount of 1‰. Incubate at room temperature for 72 hours, measure the content of ammonia nitrogen in the two kinds of sewage, and determine the denitrification ability of sewage. The specific experimental results are shown in Table 1.

[0092] Table 3 The remo...

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Abstract

The invention belongs to the technical field of water environmental biological treatment and relates to a separating screen of a bacterial strain CY003 for efficiently degrading trite nitrogen, nitrate nitrogen and ammonia nitrogen, method for testing safety and preparing bacterial solution, and application for treating domestic sewage and cultivating sewage. The bacterial strain CY003 of the invention is preserved in Chinese Type Culture Collection Center and the preserved number is CCTCC No.: M209196. According to morphological observation, physiologic biochemical analysis and 16S rDNA homology analysis, the bacterial strain CY003 is Pseudomonas stutzeri which can rapidly degrade the trite nitrogen, nitrate nitrogen and ammonia nitrogen in water and is not toxic to the animals such as silver carp, crucian, freshwater shrimp, small white mouse, and the like. At normal temperature, the Pseudomonas stutzeri can excellently degrade the nitrite and the ammonia nitrogen in actual sewage. After culturing 75 h, the Pseudomonas stutzeri can degrade 94.7% and 92.7% of trite nitrogen and ammonia nitrogen in cultivating sewage and can degrade 96.2% and 94.6% of trite nitrogen and ammonia nitrogen in domestic sewage. The invention has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of water environment microbial control, and specifically relates to the screening and application of a strain of Pseudomonas stutzeri CY003 for removing nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water bodies. technical background [0002] At present, my country has done a lot of work in the prevention and control of water pollution, but the problem of eutrophication in aquaculture water bodies and natural water bodies is still very serious. The survey shows (Jin Xiangcan et al., 1995): Most of the lakes and reservoirs in my country have reached or exceeded the level of eutrophication. The "eutrophication" of the water body makes the water body smelly, fish and other organisms die in large numbers, blue-green algae bloom, and many places have no water to drink in high temperature seasons. Excessive levels of nutrients such as nitrogen and phosphorus in water bodies are the main cause of eutr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C02F3/34C02F3/02C12R1/38
CPCY02W10/10
Inventor 袁科平李谷邹世平艾晓辉何力郑卫东
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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