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Method for producing collagen polypeptide, natural pigment and fish oil by using fish skin and fish scale

A technology for collagen polypeptides and natural pigments, which is applied in the field of producing collagen polypeptides, natural pigments and fish oil from fish skin and fish scales. It can solve the problems of not extracting natural pigments and collagen polypeptides, and achieve bright colors and high yields.

Inactive Publication Date: 2011-10-12
LINGAO ZESHITAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application number: 200810064074.5, publication number CN 101240313A, mentions the method of extracting fish scale collagen peptides from fish scales, and does not extract pigments and fish oil
Chinese patent application number: 00111079.9, publication number CN 1318634A, mentions the method of extracting fish scale collagen peptides with fish scales, and does not extract pigments and fish oil
Chinese patent application number: 200710031626.9, publication number CN 101248821A, mentions the method of extracting fish oil and fish protein peptides by enzymatic hydrolysis of fish leftovers, without extracting natural pigments and collagen peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Extract 100 kilograms of 20-mesh coarse powder of fish skin and fish scales, remove impurities, put it into an extraction tank, add 300 kilograms of purified water, 0.8 kilograms of food-grade hydrochloric acid or 0.4 kilograms of food-grade sodium hydroxide. Keep warm at 14°C to 16°C and stir for 1 hour at a stirring speed of 36 rpm. Fish oil on the liquid surface is fished at any time, and the source material for refined edible fish oil is preserved. Heat up to 54°C-56°C, add 0.5 kg of special enzyme for hydrolyzing fish skin and scale, and moisten the enzyme with purified water first. Keep warm at 54°C to 56°C and stir for 6 hours to carry out hydrolysis. Heat up to 90°C and stir for 0.5 hours to inactivate the enzyme. Cool down to about 60°C, add 2.0 kg of medicinal activated carbon, 5.0 kg of kaolin, stir for 15 minutes, and let stand for 30 minutes to absorb impurities. Extract the hydrolyzate, press filter with a pressure of 0.2Mpa through a plate and frame fi...

Embodiment 2

[0029] Extract 100 kg of 20-mesh coarse powder of fish skin and fish scale, remove impurities, put it into an extraction tank, add 300 kg of purified water, 0.8 kg of food-grade hydrochloric acid or 0.4 kg of food-grade sodium hydroxide. Stir at 16° C. for 1 hour at a stirring speed of 30 rpm. Fish oil on the liquid surface is fished at any time, and the source material for refined edible fish oil is preserved. Heat up to 54°C-56°C, add 0.45 kg of special enzyme for hydrolyzing fish skin and scale, and moisten the enzyme powder with purified water first. Keep warm at 54°C to 56°C and stir for 7 hours to carry out hydrolysis. Heat up to 90°C and stir for 0.5 hours to inactivate the enzyme. Cool down to about 60°C, add 2.0 kg of activated carbon and 5.0 kg of kaolin, stir for 15 minutes, and let stand for 30 minutes to absorb impurities. Extract the hydrolyzate, press filter with a pressure of 0.25Mpa through a plate and frame filter press, and reciprocate until the filtrate ...

Embodiment 3

[0035] Get 100 kilograms of 20-mesh coarse powder of fish skin and fish scale, remove sundries, put it into an extraction tank, add 300 kilograms of purified water, 0.8 kilograms of food-grade hydrochloric acid or 0.4 kilograms of food-grade sodium hydroxide. Stir at 15° C. for 1 hour at a stirring speed of 33 rpm. Fish oil on the liquid surface is fished at any time, and the source material for refined edible fish oil is preserved. Heat up to 54°C-56°C, add 0.5 kg of special enzyme for hydrolyzing fish skin and scale, and moisten the enzyme with purified water first. Keep warm at 54°C to 56°C. Heat up to 90°C and stir for 0.5 hours to inactivate the enzyme. Cool down to about 60°C, add 2.0 kg of activated carbon and 5.0 kg of kaolin, stir for 20 minutes, and let stand for 30 minutes to absorb impurities. Extract the hydrolyzate, press filter with a pressure of 0.25Mpa through a plate and frame filter press, and reciprocate until the filtrate is clear. The aqueous solution...

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Abstract

The invention relates to a method for producing collagen polypeptide, natural pigment and fish oil by using fish skin and fish scale. So far, a method for integrally producing the four products by taking the fish skin and the fish scale as raw materials is not provided yet. The method disclosed by the invention is characterized by comprising the following steps of: deoiling the fish skin and the fish scale at low temperature; performing composite enzyme hydrolysis; adsorbing malodorous smell by adding porcellanite and active carbon; filter-pressing; clarifying; adsorbing natural pigment by using macro-porous resin; flowing out collagen polypeptide; eluting red pigment by using acid ethanol; eluting yellow pigment by using alkaline ethanol; respectively condensing, spraying and drying the solution containing collagen polypeptide and two pigments to obtain collagen polypeptide and red pigment and yellow pigment powder; and extracting crude oil by using supercritical carbon dioxide so asto obtain edible fish oil. The method disclosed by the invention has the benefits that: the collagen polypeptide is white and inodorous and has high yield; the natural red pigment and the yellow pigment have bright colour and are steady to light, heat, acid and alkali; the fish oil is edible oil; and the four products have very important significance to human bodies and can be used as nutritious foods, health care products, foods, beverages, food additives and cosmetics.

Description

technical field [0001] The invention relates to a method for producing collagen polypeptide, natural pigment and fish oil by using fish skin and scale. Background technique [0002] my country is rich in fish resources, and the leftover fish skin and scales produced by the fish processing industry are tens of thousands of tons. Fish skin contains more than 90% protein, of which collagen accounts for about 70% of protein. Fish scales contain about 30% collagen. Fish skin and fish scales still contain 6% fish oil, natural red pigment and natural yellow pigment. There are no reported articles and patents on the comprehensive production of these four products by utilizing the mixture of fish skin and fish scales. [0003] At present, the raw materials for the production of collagen polypeptide powder mainly come from the skin and bone bonds of terrestrial mammals such as pigs, cattle, mules, and horses. Due to the deterioration of the global ecological environment, diseases ...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L1/327C09B61/00A23L17/20
CPCY02P20/54
Inventor 李世泰王清云林强
Owner LINGAO ZESHITAI BIOTECH CO LTD
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