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Technique for preparing collagen of freshwater fish skin

A technology of collagen and freshwater fish, which is applied in the fields of functional food, leather, biomedicine, cosmetics, and aquatic product processing. It can solve the problems of fishy smell, poor color and low purity of aquatic collagen, and achieve the removal of fishy smell and easy operation. The effect of convenience and simple equipment

Inactive Publication Date: 2007-12-26
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention relates to a preparation process of freshwater fish skin collagen, which overcomes the technical problems of fishy smell, low purity and poor color of traditional aquatic collagen, and provides a new method for the preparation of freshwater fish skin collagen with high efficiency, low cost and high quality. Technology

Method used

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Examples

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Effect test

example ( 1

[0018] Example (1) preparation process of grass carp skin collagen

[0019] Take the frozen grass carp skin, thaw it at room temperature, remove the scales and subcutaneous tissue on the grass carp skin, wash it with tap water, drain it, and cut it into pieces of about 1×1cm with scissors. Take 10g (calculated on a dry basis), add 200mL of 0.01M sodium hydroxide solution containing 1% (v / v) hydrogen peroxide, soak at 20°C for 24h, stir with a stirrer (Philips, HR2964) at high speed (20000rpm) for 2min, double Filter with a layer of gauze; wash the fish skin with 10% (v / v) n-butanol for 3 times, then add 200mL of 10% (v / v) n-butanol to soak for 6h, filter with gauze, and wash with tap water; then add 1.5L containing 1g Pepsin, pH 2.5, 0.5M acetic acid solution, extract at 4°C for 36 hours, filter the above system through a 50 μm membrane, then filter through a 5 μm membrane, then add 5M NaCl solution to make the final concentration 0.9M, centrifuge to obtain a precipitate Diss...

example ( 2

[0020] Example (two) herring skin collagen preparation process

[0021] Take the frozen herring skin, thaw it at room temperature, remove the scales and subcutaneous tissue on the herring skin, wash it with tap water, drain it, and cut it into pieces of about 1×1cm with scissors. Take 10g (calculated on a dry basis), add 300mL of 0.01mol / L sodium hydroxide solution containing 0.3% (v / v) hydrogen peroxide, soak at 4°C for 36h, stir with a mixer (Philips, HR2964) at high speed for 3min, double-layer Filter with gauze; wash the fish skin with ethanol for 3 times, then add 200mL of ethanol to soak for 6h, filter with gauze, and wash with tap water; then add 2L of 0.5g pepsin, pH 2.5, 0.5M acetic acid solution, extract at 20°C for 36h, and the above system After filtering with a 30 μm membrane, then filter with a 3 μm membrane, then add 5M NaCl solution to make the final salt concentration 2.0M, centrifuge to obtain a precipitate that is dissolved in 0.05mol / L acetic acid and dialy...

example ( 3

[0022] Example (three) carp skin collagen preparation process

[0023] Take the frozen carp skin, thaw it at room temperature, remove the scales and subcutaneous tissue on the carp skin, wash it with tap water, drain it, and cut it into pieces about 1×1cm with scissors. Take 10g (calculated on a dry basis), add 300mL of 0.01mol / L sodium hydroxide solution containing 2% (v / v), soak at 20°C for 24h, stir with a stirrer (Philips, HR2964) at high speed (50000rpm) for 4min, Filter with double-layer gauze; wash the fish skin with acetone for 3 times, then add 200 mL of acetone to soak for 6 h, filter with gauze, and wash with tap water; then add 3 L of pepsin, pH 2.5, 0.5 M acetic acid solution, extract at 20 ° C for 48 h, Filter the above system with a 30 μm membrane, then filter with a 2 μm membrane, then add 5M NaCl solution to a final concentration of 0.9M, centrifuge to dissolve the precipitate in 0.01M acetic acid, dialyze, and freeze-dry to obtain white and odorless carp skin...

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Abstract

This invention discloses a method for preparing freshwater fish skin collagen. The method comprises: (1) washing fish skin, draining, cutting, and soaking in 0.001-0.2 M alkali solution containing 0.005-2% (v / v) H2O2 for 3-48 h; (2) stirring at a high speed, washing and soaking with an organic reagent; (3) extracting with acid solution (pH = 1.0-5.0) containing 0.01-2% (m / v) pepsin for 12-64 h, and filtering with 30-100 mu.m membrane, and then with 1-30 mu.m membrane; (4) adding 5 M NaCl solution into the filtrate to 0.8-2.0 M, centrifuging, dissolving the precipitate in 0.001-0.1 M acetic acid, dialyzing with distilled water, and freeze-drying to obtain white and odorless collagen solid. The collagen can be widely used in food, cosmetic and biomedical fields.

Description

1. Technical field [0001] The invention relates to a preparation process of freshwater fish skin collagen, which belongs to the technical field of aquatic product processing, and the product can be widely used in the fields of functional food, biomedicine, cosmetics, leather and the like. 2. Technical background [0002] With the development of the domestic aquatic product processing industry, the leftovers such as fish heads, fish skins, fish bones, etc. produced in the process of aquatic product processing are increasing year by year. These leftovers are mainly used as low-value-added feed, fertilizer, etc., or directly discarded , causing serious environmental pollution. Therefore, it is of great significance to research, develop and utilize these low-value-added leftovers to improve their added value. [0003] Fish skin is rich in collagen, which can be used to make cosmetics and health foods that have the functions of skin care and beauty, delaying skin aging and mainta...

Claims

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Application Information

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IPC IPC(8): C07K4/12C07K1/36
CPCY02P20/582
Inventor 胡秋辉安辛欣杨方美辛志宏张建忠王林赵立艳
Owner NANJING AGRICULTURAL UNIVERSITY
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