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Preparation method of sleeve-fish skin low-molecular-weight collagen peptide

A technology of collagen peptide and low molecular weight, which is applied in the field of preparation of squid skin low molecular weight collagen peptide, can solve the problems of long preparation cycle, low protein extraction rate, difficult control of collagen molecular weight, etc., and achieve simple preparation method and uniform color , Increase the effect of added value

Active Publication Date: 2012-09-26
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its weak point is, at first this invention needs squid skin to be soaked 2-8d in NaOH aqueous solution to decolorize earlier, then in Ca(OH) 2 Soak in the solution for 2-4 days to fix the color, and the preparation cycle is longer. Secondly, the invention uses high-pressure treatment to process squid skin protein to prepare bioactive peptides. The preparation conditions are strict, and the preparation process and the molecular weight of collagen are not easy to control. In addition, This invention adopts PH neutral water as protein extractant, and the protein extraction rate is low

Method used

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  • Preparation method of sleeve-fish skin low-molecular-weight collagen peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take 1Kg of squid skin after washing and mash it with a masher; add 30L of NaOH solution with a mass fraction of 0.4% to the mashed squid skin, wash with water after soaking for 24h until the pH is neutral, then add 40L volume percent 12% n-butanol solution, stirred for 24 hours, washed with water until the pH was neutral and drained; 60L of citric acid solution with a mass fraction of 0.5% was added to the drained squid skin, heated to 60°C, and Constantly stirring and extracting for 7 hours, after filtration, the crude collagen extract was obtained; the pH of the crude collagen extract was adjusted to 8 with phosphate buffer, and then the compound enzyme was added. For the amount of the compound enzyme and the percentage content of each enzyme in the compound enzyme, see Table 1. Enzymolysis was carried out at an ultrasonic frequency of 40kHz, power of 100W, and temperature of 60°C for 2 hours; the obtained enzymolysis solution was heated to 95°C and kept for 7 minutes...

Embodiment 2

[0033]Take 1Kg of squid skin after washing and mash it with a masher; add 50L of NaOH solution with a mass fraction of 0.2% to the mashed squid skin, soak it for 30h, wash it with water until the pH is neutral, and then add 30L volume percent 10% n-butanol solution, stirred for 16 hours, washed with water until the pH is neutral and drained; 80L of 0.3% acetic acid solution was added to the drained squid skin, heated to 90°C, and continuously Stir and extract for 8 hours, and obtain the crude collagen extract after filtering; adjust the pH of the crude collagen extract to 7 with phosphate buffer, then add the compound enzyme, the amount of the compound enzyme added and the percentage content of each enzyme in the compound enzyme are shown in the table 1. Carry out enzymatic hydrolysis at an ultrasonic frequency of 30kHz, a power of 300W, and a temperature of 55°C for 1 hour; heat the obtained enzymatic solution to 90°C and keep it for 5 minutes to inactivate the enzyme. The fi...

Embodiment 3

[0039] Take 1Kg of squid skin after washing and mash it with a masher; add 35L of KOH solution with a mass fraction of 0.5% to the mashed squid skin, soak it for 28h, wash it with water until the pH is neutral, and then add 50L volume percent 15% n-butanol solution, stirred for 20 hours, washed with water until the pH is neutral and drained; 50L of citric acid solution with a mass fraction of 0.2% was added to the drained squid skin, heated to 80°C, and Constantly stirring and extracting for 6 hours, after filtration, the crude collagen extract was obtained; the pH of the crude collagen extract was adjusted to 7 with phosphate buffer solution, and then the compound enzyme was added. For the amount of the compound enzyme and the percentage content of each enzyme in the compound enzyme, see Table 1. Enzymolysis was carried out at an ultrasonic frequency of 25kHz, power of 400W, and temperature of 50°C for 1 hour; the obtained enzymolysis solution was heated to 100°C and kept for ...

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Abstract

The invention discloses a preparation method of a sleeve-fish skin low-molecular-weight collagen peptide. The preparation method adopts a compound enzyme composed of three enzymes including trypsin, papain and alkali protease, the three enzymes carry out a synergistic effect on sleeve-fish skin collagen under an ultrasonic condition to carry out enzymolysis on the sleeve-fish skin collagen; and then, steps of de-bittering, de-coloring, concentrating in vacuum, and spraying and drying can be performed to obtain the sleeve-fish skin low-molecular-weight collagen peptide. The preparation method disclosed by the invention is simple, the process is easy to control and no environmental pollution exists; the enzymolysis time is short and the production efficiency is high; the enzymolysis speed and the extracting rate of the collagen peptide can be improved; and the sleeve-fish skin low-molecular-weight collagen peptide obtained by using the preparation method is easy to digest and absorb, has no objectionable odors, uniform color and luster and good biological performance, and is relatively easy to digest and absorb by a human body.

Description

technical field [0001] The invention relates to a preparation method of collagen peptide, in particular to a preparation method of squid skin low molecular weight collagen peptide. Background technique [0002] Collagen, or collagen, is a biological polymer synthesized by animal cells. It is widely found in animal bones, tendons, cartilage, skin and other connective tissues. It has the functions of supporting organs and protecting the body. Collagen is the most abundant protein in mammals, accounting for about one-third of the total protein in animals. In recent years, with the development of collagen extraction technology, the in-depth study of its properties and structure, and the improvement of the understanding of the biological functions of collagen and collagen peptides, the research and application of collagen has become a hot spot. However, due to the unique three-strand superhelical structure of collagen, its properties are very stable, and general processing tempe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/12C07K1/34
Inventor 杨会成许丹郑斌周宇芳廖妙飞钟明杰付万冬马华威
Owner ZHEJIANG MARINE DEV RES INST
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