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Fish skin collagen based composite sponge and preparation method thereof

A technology of composite sponge and fish skin collagen is applied in the field of fish skin collagen-based composite sponge and its preparation, which can solve the problems of rapid degradation and poor flexibility, and achieve the effects of simple preparation process, good hygroscopicity, low price and easy availability

Inactive Publication Date: 2013-04-03
福建省博特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, as a biomedical material, aquatic collagen has the same disadvantages as terrestrial mammalian collagen, such as fast degradation in vivo and poor flexibility, which limit its application.

Method used

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  • Fish skin collagen based composite sponge and preparation method thereof
  • Fish skin collagen based composite sponge and preparation method thereof
  • Fish skin collagen based composite sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of fish skin collagen-based composite sponge

[0023] 1) Weigh 50g of chopped and washed silver carp skin and put it in 1000mL0.01mol / LNaOH solution (containing 1mLH 2 o 2 ), stirred and soaked at 4°C for 48h, washed with water until neutral; put the washed fish skin in 300mL distilled water and 30mL isopropanol, stirred and soaked at 4°C for 4h, washed with water until neutral; weighed 25.6gNaCl and dissolved it in 1000mL water, then soak the fish skin in NaCl solution for 10 hours, centrifuge, remove the waste liquid, and obtain the pretreated minced fish skin.

[0024] 2) Mix the above minced fish skin with 500mL deionized water, homogenate for 15min, add 1.5g pepsin, adjust the pH to 2.0, enzymatically hydrolyze at 10°C for 10h, filter, centrifuge the filtrate at 8000r / min for 20min at low temperature, and take the supernatant .

[0025] 3) Add 2% acetic acid solution to the supernatant, filter, dialyze with 2% acetic acid solution a...

Embodiment 2

[0027] Embodiment 2: the preparation of fish skin collagen-based composite sponge

[0028] 1) Weigh 50g of chopped and washed silver carp skin and put it in 1000mL0.01mol / LNaOH solution (containing 5mLH 2 o 2 ), stirred and soaked at 4°C for 24h, washed with water until neutral; put the washed fish skin in 300mL distilled water and 30mL isopropanol, stirred and soaked at 4°C for 4h, washed with water until neutral; weighed 25.6gNaCl and dissolved it in 1000mL water, then soak the fish skin in NaCl solution for 10 hours, centrifuge, remove the waste liquid, and obtain the pretreated minced fish skin.

[0029] 2) Mix the above minced fish skin with 500mL deionized water, homogenate for 15min, add 1.3g pepsin, adjust the pH to 3.0, enzymolyze at 6°C for 20h and filter, centrifuge the filtrate at 8000r / min for 20min at low temperature, and take the supernatant .

[0030] 3) Add 2% acetic acid solution to the supernatant, filter, dialyze with 2% acetic acid solution as the exter...

Embodiment 3

[0032] Embodiment 3: the preparation of fish skin collagen-based composite sponge

[0033] 1) Weigh 50g of chopped and washed silver carp skin and put it in 1000mL0.01mol / LNaOH solution (containing 10mLH 2 o 2 ), stirred and soaked at 4°C for 12h, washed with water until neutral; put the washed fish skin in 300mL distilled water and 30mL isopropanol, stirred and soaked at 4°C for 4h, washed with water until neutral; weighed 25.6gNaCl and dissolved it in 1000mL water, then soak the fish skin in NaCl solution for 10 hours, centrifuge, remove the waste liquid, and obtain the pretreated minced fish skin.

[0034] 2) Mix the above minced fish skin with 500mL deionized water, homogenate for 15min, add 1.0g pepsin, adjust the pH to 4.0, enzymolyze at 4°C for 30h and filter, centrifuge the filtrate at 8000r / min for 20min at low temperature, and take the supernatant .

[0035] 3) Add 2% acetic acid solution to the supernatant, filter, dialyze with 2% acetic acid solution as the exte...

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Abstract

The invention provides fish skin collagen based composite sponge and a preparation method thereof. The fish skin collagen based composite sponge is prepared by the steps of: based on fish skin collagen and chitosan as raw materials, freeze-drying, carrying out cross-linking, and carrying out secondary freeze drying. The composite sponge prepared by the invention can improve the disadvantages that the previous sponge is easy to degrade and the like, is simple in preparation process, is cheap and easy to obtain, and overcomes some defects of the existing collagen sponge. The fish skin collagen based composite sponge prepared by the invention has a specific three-screws structure of an I type collagen protein; a microstructure is a double-faced porous net-shaped sheet layer; and the fish skin collagen based composite sponge has good hygroscopicity, improves the degradability of the collagen sponge, is simple in preparation process and is cheap and easy to obtain, so that the fish skin collagen based composite sponge is a better biomedical material.

Description

technical field [0001] The invention relates to a fish skin collagen-based composite sponge and a preparation method thereof. Background technique [0002] Collagen is the most abundant and widely distributed protein in animals. Collagen has the characteristics of unique triple helix structure, low antigenicity, non-toxicity, good biocompatibility and biodegradability, and has been widely used in tissue engineering scaffold materials, hemostatic sponges, drug controlled release systems, gene Delivery carrier, cosmetic surgery and other biomedical fields. At present, the collagen used in China mainly comes from the skin and Achilles tendon of pigs and cattle, but with the occurrence of diseases such as mad cow disease and foot-and-mouth disease, people have questioned their safety; due to religious and customary reasons, pig-derived collagen The application of protein is limited in some regions. Therefore, there is an urgent need to seek collagen sources with better qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28
Inventor 张其清陈名懋郭豪王建华伍久林
Owner 福建省博特生物科技有限公司
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