Method for identifying mature rape honey

Abstract

The invention provides a method for identifying mature rape honey. The method comprises the following steps that: to-be-detected honey is detected by adopting liquid chromatography-quadrupole rod-time-of-flight mass spectrometry; excimer ion peaks with retention time of 8.99 + / -0.10 min and the mass number of 285.04 + / -0.01 as characteristic peaks, and when it is judged that the to-be-detected honey is mature rape honey when a detection spectrum contains the characteristic peaks. The liquid chromatography detection conditions are as follows: a C18 chromatographic column is adopted; a mobile phase A is 0.1% formic acid aqueous solution; a mobile phase B is methanol; the volume fraction of the mobile phase B is increased to 5% within 0-1 min of gradient elution; the volume fraction of the mobile phase B is increased from 5% to 55% within 1-6 min of gradient elution; the volume fraction of the mobile phase B is increased from 55% to 95% within 6-20 min of gradient elution; the volume fraction of the mobile phase B is maintained at 95% within 20-26 min of gradient elution; and the volume fraction of the mobile phase B is reduced to 5% within 26-27 min of gradient elution. The method provided by the invention can be used for identifying the mature rape honey quickly and accurately.

Description

technical field [0001] The invention belongs to the field of honey identification, in particular to a method for identifying mature rapeseed honey. Background technique [0002] Honey has long been favored by consumers due to its rich active substances and high nutritional value, but it is also threatened by honey adulteration. Typical honey adulteration behaviors are syrup blending, in addition to using ion exchange resins to decolorize honey and shoddy it, confusing honey nectar types, artificially feeding sucrose during the collection period, and harvesting immature honey to increase production. [0003] The act of collecting immature honey to increase honey production and gain higher income has also become more and more common in recent years. Ripe honey refers to honey that has been fully brewed by bees. After the bees have brewed the honey, they will store it centrally in the hive and seal it with beeswax. To get honey at this time, the honey cover must be cut off w...

AI Technical Summary

Problems solved by technology

The immature honey has not been fully brewed, the brewing time is short, the water content in the honey is high, the high molecular sugar is not completely decomposed, the content of bee enzymes is low, the honey is thin, and its nutritional value is far inferior to that of mature honey.

Unscrupulous merchants are driven by profit to collect immature honey, and in order to make the honey meet the requirements of the standard, heat and dehydrate the honey at high temperature. However, the heating process destroys the active substances in honey, especially polyphenols, and produces the products of Maillard reaction, which seriously affects the nutritional value of honey.

[0004] At present, the identification of mature honey is cumbersome, and it is not suitable for users to identify

Among them, rapeseed honey is the largest and stable honey species, accounting for more than 40% of the total honey production throughout the year. However, the vast majority of rapeseed honey on the market is obtained by processing and concentrating immature honey, which seriously disrupts market order and damages consumer interest

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