Honey detection composition, kit, detection method and application

The technology of a composition and a kit is applied in the direction of biochemical equipment and methods, and the determination/testing of microorganisms, so as to achieve reliable results, simple operation, and avoid false positive results

Inactive Publication Date: 2013-07-24
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this type of adulteration, traditional detection methods, such as sensory methods, physical properties, chemical composition analysis, etc., have many uncertainties due to the influence of production, processing, and storage conditions.

Method used

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  • Honey detection composition, kit, detection method and application
  • Honey detection composition, kit, detection method and application
  • Honey detection composition, kit, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this example, the specificity and sensitivity of the primers and probes of litchi, longan, acacia, rape, and longan were verified through the following tests.

[0038] Detection of the main reagents:

[0039] Chloroform and isopropanol were respectively purchased from Beijing Liuhetong Company; CTAB lysate (20g / L CTAB, 1.4mol / L NaCl, 0.1mol / L Tris, 0.02mol / L NaCl 2 -EDTA), CTAB precipitation solution (5g / L CTAB, 0.04mol / L NaCl), and 1.2mol / L NaCl were self-prepared in this experiment; Fast Start Universal Probe MasterMix (Rox) was purchased from Roche; primers and probes were purchased from Synthesis by Shanghai Aoke Biotechnology Co., Ltd., etc.

[0040] The main steps of detection:

[0041] 1) DNA extraction

[0042] Test samples: 30 samples, including acacia, litchi, rape, milk vetch, pear, apple, peach, soybean, barley, rice, cucumber, carrot, longan, orange, Vitex, ginkgo, loquat, jujube, wolfberry, rose, Bee, dog, deer, camel, horse, cow, duck, sheep, fish,...

Embodiment 2

[0070] The inventors of the present invention verified the collected honey samples through the following tests.

[0071] Rapeseed honey, litchi honey, acacia honey, longan honey and their mixtures were selected for real-time fluorescent PCR reaction to determine the feasibility, specificity and actual sensitivity of the established real-time fluorescent PCR method.

[0072] In this example, the specific probes used and the experimental operating conditions for real-time fluorescent PCR detection are as described in Example 1.

[0073] Conventionally, when the sample to be tested is amplified, if more than one positive signal occurs three times, it means that the test result is positive.

[0074] Take the mixture of lychee honey and rapeseed honey as an example, mix lychee honey and rapeseed honey in different proportions. Such as Figure 9 As shown, the proportions of litchi honey and rapeseed honey were 100%, 99.9%, 90%, 50%, 10%, 5%, 1% and 0.1%, respectively. Using the N...

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PUM

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Abstract

The invention relates to an oligonucleotide primer and a probe for detecting the longan, lychee, locust and rape honey plant components in honey, a kit comprising the primer and the probe, and a method for detecting the honey plant components in honey. According to the invention, the honey plant components in honey can be simply, quickly, specifically and sensitively detected.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to oligonucleotide primers and probes for detecting nectar-derived plant components in honey, kits containing the primers and probes, and the use of the primers and probes. Method for detection of nectar-derived botanical constituents in honey by needle or kit. The present invention also relates to the application of the primers and probes in the detection of nectar plant components in honey. Background technique [0002] Honey is a natural sweet substance that is fully brewed after bees collect nectar, secretions or honeydew from plants, combine with their own secretions. There are many kinds of nectar source plants, such as rapeseed honey, Sophora japonica honey, Vitex twig honey, jujube honey, longan honey, litchi honey and so on. The prices of honey from different nectar sources vary greatly, with high-end prices such as longan honey and lychee honey, and lo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 吴亚君陈颖王斌刘明畅韩建勋
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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