Identification method of chaste honey, rape honey and acacia honey based on NMR (Nuclear Magnetic Resonance) technique

Abstract

The invention discloses an identification method of chaste honey, rape honey and acacia honey based on an NMR (Nuclear Magnetic Resonance) technique. The identification method comprises the followingsteps: filtering a honey sample and then preparing the filtered honey sample into 0.03 to 0.5 g/L solution with heavy water; adding a phosphate buffered solution with the concentration of 0.5 to 2.0 mol/L, wherein the phosphate buffered solution contains a 3-trimethylsilyl-1-sodium propyl sulfonate internal standard solution with the volume fraction of 0.05 to 0.3 percent and 0.1 to 3.0 mM of sodium azide; mixing, adjusting the pH value of the phosphate buffered solution to 2.5 to 4.0 and measuring by a NMR spectrometer to obtain an NMR spectrum; and analyzing chemical shift of a honey samplemarker, and determining that the honey sample of which 1H chemical shift delta is 7.0 to 7.5 ppm and a deviation delta is within a range of 0.02 to 0.05 ppm is the chaste honey, otherwise the honey sample is the rape honey or the acacia honey. The method has the advantages that a large quantity of samples can be analyzed in a short time and the chaste honey, the rape honey and the acacia honey canbe quickly identified.

Description

technical field [0001] The present invention relates to the detection of food, and more specifically relates to a method for identifying wattle honey, rapeseed honey and acacia honey based on nuclear magnetic resonance technology. Background technique [0002] The national standard for honey GB14963-2011GB stipulates that honey is a natural sweet substance that is fully brewed after the nectar, secretion or honeydew collected by bees from plants, mixed with their own secretions, and is a nutritious natural nourishment. food. The flower source is different, that is, the metabolism of the plant is different, so the components of the nectar collected by the bees will be different. Honey is usually classified according to the nectar source plant, and can be divided into rapeseed honey, acacia honey, wattle honey, jujube honey, linden tree honey, etc. . In recent years, the demand for honey in the domestic and international markets has been expanding, but the output of honey is...

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Problems solved by technology

However, these methods lack sensitive and reliable markers, and their accuracy, sensit

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