Method for identifying linden honey, vicia villosa Roth honey and rape honey

A technology of rapeseed honey and linden honey, which is applied in the field of food science, can solve the problems of counterfeiting and difficult sensory identification, etc., and achieve the effects of low detection limit, good separation effect and good classification effect

Inactive Publication Date: 2015-11-11
NORTHWEST UNIV(CN)
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  • Abstract
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Problems solved by technology

However, the color and shape of rapeseed honey after crystallization are very similar to the above two kinds of honey, so it is difficult to distinguish with the senses, and it is often used to pretend to be high-priced honey

Method used

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  • Method for identifying linden honey, vicia villosa Roth honey and rape honey
  • Method for identifying linden honey, vicia villosa Roth honey and rape honey
  • Method for identifying linden honey, vicia villosa Roth honey and rape honey

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Embodiment Construction

[0023] Further description will be given below in conjunction with the accompanying drawings and embodiments.

[0024] Weigh 10g each of linden honey (14 batches with numbers L1-L14), jasmine honey (11 batches with numbers V1-V11) and rapeseed honey (15 batches with numbers R1-R15) from different origins, and put them in 100mL In a beaker, add 60 mL of distilled water to fully dissolve and then adjust the pH to 2 with HCl; inject the honey sample aqueous solution into a chromatographic column (30 cm × 2.5 cm) equipped with Amberlite XAD? HCl) aqueous solution, elute with 200mL distilled water to remove polar substances such as sugars, then elute the phenolic compounds adsorbed on the resin with 200mL methanol, collect methanol, concentrate under reduced pressure at 42°C to dryness, and dilute the residue with chromatographically pure methanol to 2mL, the resulting solution was filtered through a 0.45μm microporous membrane as the test sample solution, and placed in a sealed pl...

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Abstract

The invention discloses a method for effectively identifying linden honey, vicia villosa Roth honey and rope honey. The method comprises the following steps: firstly extracting a phenolic compound from honey samples by using XAD-2 type resin, carrying out HPLC-ECD (High Performance Liquid Chromatography-Electrochemical Detector) analysis, establishing HPLC-ECD fingerprint spectrums of the linden honey sample, the vicia villosa Roth honey sample and the rape honey sample by using obtained HPLC-ECD spectrum data, and analyzing the fingerprint spectrums of the linden honey sample, the vicia villosa Roth honey sample and the rape honey sample to obtain a large amount of common peak information; carrying out analysis and comparison on the common peak information, then picking out 17 common peaks in total as characteristic common peaks, and classifying the three kinds of honeys by combining a chemometrics method. The method disclosed by the invention is reasonable in design; selected analysis instruments are high in sensitivity, good in separation effect and high in feasibility; an important technical problem in quality control of the linden honey and the vicia villosa Roth honey is solved.

Description

technical field [0001] The invention relates to identification methods of three kinds of light-colored easy-to-crystallize honeys (linden tree honey, broom honey and rapeseed honey) specially produced in China, and belongs to the field of food science. Background technique [0002] Honey is a natural sweet substance that is repeatedly processed and brewed by bees after collecting the secretions of the intrafloral nectary or extrafloral nectary of the nectar source plant under the action of bee substances and enzymes. In addition to the main components such as glucose and fructose, honey also contains trace active substances such as phenols, proteins, amino acids and organic acids that are closely related to the types of honey plants. Tumor, cardiovascular protection and other effects. The chemical composition, color and taste of honey from different plant sources will be different, and these differences will affect its sales. [0003] Linden honey (Lindenhoney), scorpion h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 赵静曹炜程妮吕新刚
Owner NORTHWEST UNIV(CN)
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