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Method based on three-dimensional fluorescence spectrum technology to quickly recognizing and quantitatively analyzing false honey

A three-dimensional fluorescence and spectroscopic technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of pure honey affecting quantitative analysis of honey, and achieve the effects of good use value, simple algorithm, and high prediction accuracy.

Inactive Publication Date: 2018-08-07
TIANJIN POLYTECHNIC UNIV
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Problems solved by technology

[0024] The purpose of the present invention is to propose a method for rapid identification and quantitative analysis of adulterated honey based on three-dimensional fluorescence spectroscopy, to solve the problem that the quenching effect of pure honey affects the quantitative analysis of honey, and to find the best concentration dilution point for honey measurement , and use this method to accurately measure the content of each ingredient in honey mixed with corn syrup

Method used

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  • Method based on three-dimensional fluorescence spectrum technology to quickly recognizing and quantitatively analyzing false honey
  • Method based on three-dimensional fluorescence spectrum technology to quickly recognizing and quantitatively analyzing false honey
  • Method based on three-dimensional fluorescence spectrum technology to quickly recognizing and quantitatively analyzing false honey

Examples

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example 1

[0043] Taking the detection of acacia honey mixed with corn syrup (f90) as an example, the specific detection method is as follows:

[0044] Step 1. Accurately measure 1ml each of pure acacia honey and corn syrup (f90) and transfer them to 100ml volumetric flasks, add distilled water to the mark, and bathe in water for 5 minutes, and use it as a spare solution and a predicted standard solution. Pipette 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml solution from the diluted acacia honey stock solution to a 10ml volumetric flask, add corn syrup (f90) stock solution to the mark Line, shake evenly, and configure it as the solution to be tested; take 1ml of each solution to be tested and put it into a fluorescence cuvette, put it at room temperature for about 40 minutes, and use a fluorescence spectrophotometer to perform fluorescence scanning. The parameters are set as follows: the excitation wavelength is 240 ~360nm, the emission wavelength is 260~800nm, the scanning step length is...

example 2

[0049] Taking the detection of acacia honey samples mixed with corn syrup (f90) and corn syrup (f42) as an example, the specific detection methods are as follows:

[0050] Step 1. Accurately measure 1ml each of pure acacia honey, corn syrup (f90) and corn syrup (f42) into 100ml volumetric flasks, add distilled water to the mark, and bathe in water for 5 minutes, as a reserve solution and prediction standard liquid use. Pipette 6ml, 5ml, and 3ml of solution from the diluted acacia honey stock solution to a 10ml volumetric flask, then add 2ml, 3ml, and 4ml of corn syrup (f90) to the flask, and finally add corn to the volumetric flask Syrup (f42) to the scale line, shake evenly, and prepare the solution to be tested; take 1ml each of the solution to be tested and the standard solution, put them in a fluorescent cuvette, put it at room temperature for about 40 minutes, and use a fluorescence spectrophotometer for fluorescence scanning , the parameters are set as follows: the exci...

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Abstract

Accurate quantitative analysis on false honey has important significance on quality evaluation of the false honey. Therefore, the invention discloses a novel detection method based on three-dimensional fluorescence characteristics of honey and corn syrup. When the honey and the syrup are diluted to 1% (v:v), no fluorescence quenching effect is found in two observation wavelength ranges of an excitation wavelength ex 240 to 320nm and an excitation wavelength x 320 to 360nm, and fluorescence intensity and concentration are in linear relation. Under the situation, a spectral additive principle isutilized to estimate the purity of the false honey; namely, when a volume ratio concentration of the honey and the syrup is 1%, a spectrum of the false honey is compared with a spectrum of the honeyand the syrup. The method can be used to detect robinia honey in which f42 corn syrup and f90 corn syrup are doped, and a prediction error is 5.85; results show that the method can accurately estimatea content of the corn syrup in the robinia honey, honey quality in honey market is further improved, and a theory basis is provided for researching accurate and convenient detection equipment later.

Description

technical field [0001] The invention belongs to the field of spectrum analysis and discloses a method for quickly identifying and quantitatively analyzing adulterated honey based on three-dimensional fluorescence spectrum technology. Background technique [0002] Honey is a natural nourishing food with high nutritional value. It has been used as a part of traditional medicine since ancient times. It has antibacterial, anti-tumor, anti-inflammatory, anti-browning, anti-viral and other effects [1] . However, with the development of science and technology, honey adulteration has become more and more common and high-end. The honey market is relatively chaotic, and the adulteration phenomenon is serious. Unqualified samples have been detected all over the country. The identification of honey adulteration is imminent. Scholars at home and abroad are exploring new methods of detecting honey. At present, the main detection techniques include enzyme activity assays [2] , isoenzyme...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 白华丛琳
Owner TIANJIN POLYTECHNIC UNIV
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