Method for rapidly identifying single-flower honeys by using capillary electrophoresis technology

Abstract

The invention discloses a method for rapidly identifying single-flower honeys by using a capillary electrophoresis technology. The method is used for analyzing proteins in honeys by using the capillary electrophoresis technology and identifying authenticity of honeys by using difference of content and types of proteins in different honeys. The method is simple and convenient, is high in efficiencyand accurate in identification results, and has an extensive application prospect in true and false identification of the honeys. The method comprises the following steps: (1) adding sample bufferingliquid into a honey sample for pretreating, reacting for one hour at normal temperature, then centrifuging for 5 minutes at 10000rpm to obtain supernatant liquid which is a sample; (2) carrying out capillary zone electrophoresis on the sample and establishing capillary electrophoresis fingerprint spectrums of single-flower honeys; and (3) carrying out chemometrics analysis of the fingerprint spectrums and achieving identification of the single-flower honeys. The minimum detection limit of the method on a jujube flower honey, a chaste honey and a wolfberry honey is 5%; the minimum detection limit of the method on a robinia honey is 10%.

Description

technical field [0001] The invention belongs to the field of food quality and safety, and in particular relates to a method for rapidly identifying unifloral honey by using capillary electrophoresis technology. Background technique [0002] Honey refers to the natural sweet substance that bees collect the nectar, secretion or honeydew of plants, mix with their own secretions, and fully brew. Single-floral honey is the honey brewed by bees from the nectar of a single plant. Its taste, nutritional value, physiological efficacy and breeding cost are all higher than mixed-floral honey. In order to seek profit, unscrupulous businessmen often replace single flower honey with cheap miscellaneous flower honey. At present, the authenticity identification of single-floral honey has increasingly become a research hotspot for scholars in the field of food safety at home and abroad. [0003] The traditional identification methods of honey authenticity include sensory identification, ph...

AI Technical Summary

Problems solved by technology

At present, most of these methods use sugar, polyphenols and other substances with high content in honey for identification. These substances have low cost of adulteration and are easy to be adulterated. It is impossible to avoid honey from the root cause. At the same time, there are generally complex pretreatment steps, The detection speed is slow, the sensitivity and the accuracy are not high, therefore, it is urgent to establish a fast, simple and efficient single-floral honey detection method to effectively regulate the honey market

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