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Method for establishing tissue culture rapid propagation system of single-leaf robinia. pseudoacacia

A technology of tissue culture and rapid propagation of black locust single leaf, which is applied in the field of plant tissue culture, and can solve the problems of not being able to meet the needs of black locust seedlings, low reproduction coefficient, and long cycle

Inactive Publication Date: 2015-06-10
杨业云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, black locust seedlings are mainly propagated by cutting method, root burying method, grafting method and sowing method. Due to the low reproduction coefficient and long cycle of these methods, they are far from meeting the needs of large-scale afforestation for black locust seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Start-up culture: Select the annual lignified stem section with buds of robust Robinia unileaf plant as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds, and wash them with washing powder water first. Rinse for 1 hour, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 1 hour, then place it in an ultra-clean workbench, first sterilize it with 75% ethanol for 5 seconds, and then use a sterile Wash 3 times with water, then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse 4 times with sterile water, dry the water droplets on the surface with sterile filter paper, inoculate into the starting medium for induction culture, and inoculate at 28°C after inoculation. Cultivate in total darkness for 2 days, then place in the light for 10 hours per day, and cultivate for 5 days under the condition of light intensity of 1500lx to s...

Embodiment 2

[0021] (1) Start-up culture: Select the annual lignified stem section with buds of robust Robinia unileaf plant as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds, and wash them with washing powder water first. Rinse for 1 hour, then gently brush the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 2 hours, then place it in an ultra-clean workbench, first sterilize it with 75% ethanol for 8 seconds, and then use a sterile Wash 3 times with water, then sterilize with 0.1% mercuric chloride solution for 15 minutes, rinse 4 times with sterile water, dry the water droplets on the surface with sterile filter paper, inoculate into the starting medium for induction culture, and inoculate at 28°C after inoculation. Cultivate in total darkness for 2 days, and then place it in the light for 11 hours a day, and cultivate for 7 days under the condition of light intensity of 1500l...

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PUM

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Abstract

The invention discloses a method for establishing a tissue culture rapid propagation system of single-leaf robinia. pseudoacacia, and relates to a method for rapidly obtaining lots of good-quality robinia. pseudoacacia seedlings from the robinia. pseudoacacia in short time by use of a plant tissue culture technique. According to the method, the tissue culture rapid propagation system of the single-leaf robinia. pseudoacacia is established by taking the annual lignified stem with buds of the single-leaf robinia. pseudoacacia as explants and by virtue of the processes of starting of culture, subculture and multiplication culture, strong seedling and rooting culture, acclimatization and transplant and the like; as a result, technical support is provided for the large-scale production of the single-leaf robinia. pseudoacacia and a foundation is laid for further molecular breeding.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a method for establishing a tissue culture and rapid propagation system of Robinia monophylla. Background technique [0002] locust( Robinia. pseudoacacia ) is a deciduous tree of the genus Robinia in the leguminous family. It is a good timber, water and soil conservation, windbreak and sand fixation, soil improvement and "surrounding" greening tree species. Black locust is native to the United States and is naturally distributed in the Appalachian and Ozark Mountains in the eastern United States. Because of its strong adaptability and fast-growing ability, it has been introduced and cultivated by many countries. It is called together with poplar and eucalyptus. One of the three most successful tree species introduced in the world. Since it was introduced into my country in 1877, black locust has been localized in China and formed many local types....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨业云
Owner 杨业云
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