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Method for carrying out breeding crossbred crop by using male sterile line in type of nucleo-cytoplasmic interaction

A male sterile line and gene restoration technology, applied in the fields of botany equipment and methods, plant gene improvement, application, etc., can solve the problem of unsatisfactory outcrossing habits, poor recoverability, low pollen fertility of hybrids, etc. question

Inactive Publication Date: 2007-10-10
HUNAN HYBRID RICE RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inventors of the present invention are in order to solve the problem of poor recoverability of the nuclear-cytoplasmic interaction male sterile line existing in the utilization of heterosis, unsatisfactory outcrossing habits, low pollen fertility of hybrid species, and greater environmental impact on fruiting. , and the confidentiality problem of the sterile line and propose and complete the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 Preparation of Fengyuan A / Fengyuan B' / / Shu Hui 527 combination

[0053] CMS line A: Fengyuan A, a male sterile line of indica rice saccharoplast interaction type (Hybrid Rice, 2006 21(1): 51-53);

[0054] Maintainer line B: Fengyuan B, the nucleus is the same as that of Fengyuan A.

[0055] Restorer line R: Shuhui 527 (Hybrid Rice, 2004, 19(4): 12-14)

[0056] Secondary restorer gene providing parent: Minghui 63, a strong restorer line of wild-type indica rice (Crop Research, 2004, 18(4): 26)

[0057] 1) Fengyuan A was crossed with Minghui 63 and backcrossed with Fengyuan A to obtain hybrid seeds of Fengyuan A / / Fengyuan A / Minghui 63. in F 1 A single plant with high fruiting was selected in the population, and Fengyuan A was used as the male parent to backcross to obtain the hybrid seeds of Fengyuan A / / / Fengyuan A / / Fengyuan A / Minghui 63.

[0058] 2) F in Fengyuan A / / / Fengyuan A / / Fengyuan A / Minghui 63 1 A single plant with high fruiting was selected in t...

Embodiment 2

[0065] Example 2 Preparation of Xiang 8A / Xiang 8 B' / / Xiang 8B " / / / Shuhui 527 combination

[0066] Xieqingzao A is a male sterile line of nuclear-cytoplasmic interaction type rice, and Linglun is a rice line with a minor restorer gene. We found that Xieqingzao A / zero round F 1 Only showed 0.12% pollen fertility, self-fertilization was 0, completely backcrossed zero-round nuclei to Xieqingzao A cytoplasm, showed complete male fertility. It shows that under heterozygous condition, the minor restorer gene of round zero basically does not play a role in male fertility, but under homozygous condition, its role can lead to complete male fertility.

[0067] We used 121 SSR molecular markers that are relatively evenly distributed on the chromosome to be used in 232 Xieqingzao A / Zero round / / / Xieqingzao A / Zero round / / Zero round hybrid F 1 In the plants, 6 minor restorer genes were found in zero rounds, distributed on chromosomes 2, 5, 6, 8, 10, and 12. They were respectively associated...

Embodiment 3

[0084] Example 3 Preparation of Caiapo A / CaiapoB' / / CaiapoB" / / / Guangqin 26 Narrow Early Combination

[0085] CMS A: Caiapo A. The nuclei are identical to those of Caiapo B. Caiapo A is a japonica rice sclerotinia interaction male sterile line, which has poor flowering habit, 43% leaf closure rate, late flowering, scattered flowering time, and cannot be used for commercial seed production. Crossed with wide-compatibility restorer line wide-compatibility 26 narrow-early cross, F 1 Firmness was only 67.3%.

[0086] Maintainer line B: CaiapoB, Java-type japonica rice variety. (Theoretical and Applied Genetics, 2001, 102(1): 41-52).

[0087] Restorer line R: Guangqin 26 Narrowzao (Hybrid Rice, 1996, 7(6): 4-7)

[0088] Secondary restorer gene providing parent: Minghui 63, a strong restorer line of wild-type indica rice (Crop Research, 2004, 18(4): 26)

[0089] 1) Preparation of Caiapo B'

[0090] 1) Hybrid seeds of Caiapo A / / CaiapoA / Minghui 63 were obtained by crossing Caiapo...

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Abstract

A method for preparing the seeds of hybrid crops by using the minor restoring gene of nuclear-cytoplasm interactive male sterility line includes such steps as choosing the nuclear-cytoplasm interactive male sterility line A, its maintenance line B and its recovery line R, preparing the subisopolar gene line B' or B' of said B and with the minor restoring gene, discriminating the fully male sterility A / B' F1 or A / B' / / B' F1, and using A / B' / / R or A / B' / / B' / / / R to produce the seeds of hybrid crops.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular, the invention relates to a method for breeding hybrid crops using a secondary restorer gene of a nuclear-plasma interaction male sterile line. Background technique [0002] People have already discovered the phenomenon of heterosis in major crops, and it has been widely used in the actual production of crops such as corn, sorghum, rice, and rapeseed. There are many defects in the existing methods of utilizing heterosis in crops, and it is very difficult to breed practical excellent combinations, which affects the exertion of the potential of heterosis. Among the major crops, wheat, cotton, barley, soybean, etc. have hardly utilized heterosis. Improving combining ability, reducing the cost of seed production, and improving the breeding efficiency of strong and superior combinations are the development directions of the research on the utilization of crop heterosis. [0003] Three-lin...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06A01H1/00
Inventor 李新奇赵昌平袁隆平
Owner HUNAN HYBRID RICE RES CENT
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