Breeding method of polima genic-cytoplasmic male sterile line

Abstract

The invention discloses a breeding method of a polima genic-cytoplasmic male sterile line. The method mainly comprises the steps of obtaining a cytoplasmic male sterile plant by hybridizing, backcrossing, multiple crossing and an anther culture method; performing hybridizing, multi-generation backcrossing or multi-generation sister-crossing with strains with restoring gene, preparing a target sterile line and a target maintainer line; taking the target maintainer line as a recurrent parent, performing hybridizing and backcrossing with strains with fertile cytoplasm; replacing the sterile cytoplasm of the target maintainer line with fertile cytoplasm; after selfing of the cytoplasm-replaced target maintainer, performing test-crossing with the target sterile line; and identifying sterile maintaining ability of the maintainer plant and breeding the sterile line. The method can effectively remove minor restoring gene, improve outcrossing ability of the sterile line, improve breeding efficiency of the male sterile line, obtain excellent sterile line, breed full maintenance line from the sterile cytoplasmic resource which is difficult to abort thoroughly, expand utilization range of the polima genic-cytoplasmic male sterile resource and enlarge restoring spectrum of the male sterile line.

Description

technical field [0001] The invention relates to a method for breeding hybrid crops, in particular to a method for breeding male sterile lines of hybrid crops in which a nuclear-plasma interaction male sterile line system uses sterile cytoplasm as a selection background. Background technique [0002] Hybrid self-pollinated plants have long been found to have heterosis, including many desirable traits. Male sterile lines have been widely valued in the field of breeding because they can avoid manual emasculation, save manpower, reduce seed costs, and ensure seed purity. Reducing the cost of hybrid seed production, increasing the degree of freedom in combination, increasing the level of heterosis, and breeding various types of excellent hybrid combinations required for production are the development trends of crop heterosis utilization. [0003] The three-line method based on the male sterile line of nucleoplasmic interaction is an effective way to utilize heterosis. The disad...

AI Technical Summary

Problems solved by technology

The disadvantage of heterosis in the three-line method is that the available parents are limited

Therefore, the cycle of CMS breeding is quite long, difficult and predictable.