Method for acquiring heterogeneity interspecies-cloned yak premium embryos

A yak and embryo technology, which is applied in the field of preparation and in vitro culture of yak-bovine xenogeneic cloned embryos, can solve the problems of harmful embryo development, complex chemical composition, unfavorable research on embryo development dynamics, etc., and achieves the effect of improving the formation rate
CN103409467AInactive Publication Date: 2013-11-27SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHWEST UNIVERSITY FOR NATIONALITIES
Publication Date
2013-11-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation and in-vitro culture method of interspecies-cloned yak embryos. According to the method, yak-cattle interspecies-cloned embryos are constructed by using yak mammary glandular cells as donor cells and cattle or milch cow enucleated oocytes as receptor cytoplasts by a somatic nucleus transplantation technique; and an electrical activation-chemical activation-reelectrical activation mode and a self-made sequential culture system are utilized to acquire the high-efficiency high-activity yak-cattle interspecies-cloned embryos. The method disclosed by the invention can increase the yak breeding speed, shorten the yak breeding cycle, lower the yak production cost, enhance the breeding efficiency, reduce the damage level of the grassland to some extent, and effectively protect the species resource of the yak. Besides, the invention has important meanings for promoting research of interspecies-cloned yak, transgenic cloned yak embryos and nucleo-cytoplasmic interaction.
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Description

technical field

[0001] The invention relates to animal reproduction, animal embryos and animal somatic cell cloning techniques, in particular to the preparation and in vitro culture method of yak-bovine heterogeneous cloned embryos. Background technique

[0002] Yak is a unique heritage resource in my country’s Qinghai-Tibet Plateau and its surrounding areas. It is a dominant population that can adapt to harsh conditions such as severe cold, low pressure, low oxygen, and strong radiation in high-altitude areas (3500m and above), and can make full use of alpine grasslands for production. Distributed in Qinghai, Gansu, Tibet, Sichuan, Yunnan and other alpine grassland areas in China, it has become the most important means of production and living in alpine pastoral areas. Due to the special geographical location and environmental factors, the physiological characteristics of yaks include slow growth, late sexual maturity, and low fecundity, which lead to defects such as slow ge...

Claims

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