Method for acquiring heterogeneity interspecies-cloned yak premium embryos

Abstract

The invention discloses a preparation and in-vitro culture method of interspecies-cloned yak embryos. According to the method, yak-cattle interspecies-cloned embryos are constructed by using yak mammary glandular cells as donor cells and cattle or milch cow enucleated oocytes as receptor cytoplasts by a somatic nucleus transplantation technique; and an electrical activation-chemical activation-reelectrical activation mode and a self-made sequential culture system are utilized to acquire the high-efficiency high-activity yak-cattle interspecies-cloned embryos. The method disclosed by the invention can increase the yak breeding speed, shorten the yak breeding cycle, lower the yak production cost, enhance the breeding efficiency, reduce the damage level of the grassland to some extent, and effectively protect the species resource of the yak. Besides, the invention has important meanings for promoting research of interspecies-cloned yak, transgenic cloned yak embryos and nucleo-cytoplasmic interaction.

Description

technical field [0001] The invention relates to animal reproduction, animal embryos and animal somatic cell cloning techniques, in particular to the preparation and in vitro culture method of yak-bovine heterogeneous cloned embryos. Background technique [0002] Yak is a unique heritage resource in my country’s Qinghai-Tibet Plateau and its surrounding areas. It is a dominant population that can adapt to harsh conditions such as severe cold, low pressure, low oxygen, and strong radiation in high-altitude areas (3500m and above), and can make full use of alpine grasslands for production. Distributed in Qinghai, Gansu, Tibet, Sichuan, Yunnan and other alpine grassland areas in China, it has become the most important means of production and living in alpine pastoral areas. Due to the special geographical location and environmental factors, the physiological characteristics of yaks include slow growth, late sexual maturity, and low fecundity, which lead to defects such as slow ge...

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that serum or serum albumin is harmful to the development of early embryos, and may change the metabolism, ultrastructure, methylation status of imprinted genes, and fetal hypertrophy syndrome

Whether it is serum or serum albumin, the chemical composition is relatively complex, and there are many unknown factors, which is not conducive to the study of the kinetics of embryonic development in vitro

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