Method for acquiring heterogeneity interspecies-cloned yak premium embryos

A yak and embryo technology, which is applied in the field of preparation and in vitro culture of yak-bovine xenogeneic cloned embryos, can solve the problems of harmful embryo development, complex chemical composition, unfavorable research on embryo development dynamics, etc., and achieves the effect of improving the formation rate

Inactive Publication Date: 2013-11-27
SOUTHWEST UNIVERSITY FOR NATIONALITIES
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that serum or serum albumin is harmful to the development of early embryos, and may change the metabolism, ultrastructure, methylation status of imprinted genes, and fetal hype

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for acquiring heterogeneity interspecies-cloned yak premium embryos
  • Method for acquiring heterogeneity interspecies-cloned yak premium embryos
  • Method for acquiring heterogeneity interspecies-cloned yak premium embryos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The present invention will be described in detail below in conjunction with specific embodiments, and the present invention will be further described.

[0035] 1. Reagents and culture medium used

[0036] All reagents used in this study were cell culture grade products of Sigma Company unless otherwise specified. DF12, fetal bovine serum and BSA are Giboco products. Oocyte maturation fluid is TCM 199 (Giboco) containing: glutamine 0.039g / L, NaHCO 3 2.1~2.3g / L, sodium pyruvate 0.22g / L, fetal bovine serum 10%, 17β estradiol 1μg / mL, follicle-stimulating hormone 5μg / mL, luteinizing hormone 5μg / mL, epidermal growth factor 10μg / mL, insulin Growth factor 10μg / mL, pH=7.2. Embryo culture medium S1 is the basic culture medium BCM with 1.5% sodium lactate and sodium pyruvate 0.1g / L added; embryo in vitro culture medium S2 is the basic culture medium BCM with D-glucose 0.6g / L added.

[0037]The composition of the basal culture medium BCM is NaCl 6.2-6.4g / L, KCl 0.53-0.55g / L, KH...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation and in-vitro culture method of interspecies-cloned yak embryos. According to the method, yak-cattle interspecies-cloned embryos are constructed by using yak mammary glandular cells as donor cells and cattle or milch cow enucleated oocytes as receptor cytoplasts by a somatic nucleus transplantation technique; and an electrical activation-chemical activation-reelectrical activation mode and a self-made sequential culture system are utilized to acquire the high-efficiency high-activity yak-cattle interspecies-cloned embryos. The method disclosed by the invention can increase the yak breeding speed, shorten the yak breeding cycle, lower the yak production cost, enhance the breeding efficiency, reduce the damage level of the grassland to some extent, and effectively protect the species resource of the yak. Besides, the invention has important meanings for promoting research of interspecies-cloned yak, transgenic cloned yak embryos and nucleo-cytoplasmic interaction.

Description

technical field [0001] The invention relates to animal reproduction, animal embryos and animal somatic cell cloning techniques, in particular to the preparation and in vitro culture method of yak-bovine heterogeneous cloned embryos. Background technique [0002] Yak is a unique heritage resource in my country’s Qinghai-Tibet Plateau and its surrounding areas. It is a dominant population that can adapt to harsh conditions such as severe cold, low pressure, low oxygen, and strong radiation in high-altitude areas (3500m and above), and can make full use of alpine grasslands for production. Distributed in Qinghai, Gansu, Tibet, Sichuan, Yunnan and other alpine grassland areas in China, it has become the most important means of production and living in alpine pastoral areas. Due to the special geographical location and environmental factors, the physiological characteristics of yaks include slow growth, late sexual maturity, and low fecundity, which lead to defects such as slow ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/877
Inventor 熊显荣李键字向东钟金城兰道亮王永
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap