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Method for reproducing and production of hybrid seeds of rice karyoplasmic interaction type incomplete male sterility line

A male sterile line and complete technology, applied in the field of breeding, can solve problems such as unstable sterility, achieve high outcrossing rate, good combining ability, and solve the problem of strong and superior combination breeding

Inactive Publication Date: 2007-10-03
HUNAN HYBRID RICE RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, it is necessary to find an effective breeding and seed production method that can control the sterility of the nuclear-plasma interaction type incomplete male sterile line, thereby solving the problem of unstable sterility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Breeding of indica red lotus type Yuetai A in Changsha

[0030] Female parent: Yuetai A (Hybrid Rice, 1998, 13(4): 6-8), the critical temperature for the fertility transformation of Yuetai A incomplete male sterile line with nuclear-cytoplasmic interaction is the daily average temperature of 27-28 ℃.

[0031] Male parent: Yuetai B, the nucleus is the same as that of Yuetai A

[0032] Determine the seed production base. Choose a large-scale paddy field under large and medium-sized reservoirs in Changsha, with sufficient sunlight, few pests and diseases, fertile soil, and convenient transportation. It is required to ensure cold water supply. Within 100 meters around the planting field, there are no other rice varieties except the male parent. ,

[0033] Choose the best heading and flowering period. The daily average temperature is 24-30℃, the flowering time temperature is 28-32℃, and the relative humidity is 70%-90%. There is no rain for three consecutive da...

Embodiment 2

[0043] Example 2 Seed Production of Indica Hybrid Combination Xiangxiang 2A / Minghui 63

[0044] Female parent: Xiangxiang 2A (Shaoshendao No. 200408, Shaoguan City, Guangdong Province). The critical temperature for the fertility transformation of the nuclear-cytoplasmic interaction incomplete male sterile line Xiangxiang 2A is 24-25°C on average daily temperature.

[0045] Male parent: Minghui 63, a strong restorer line of wild-type indica rice (Crop Research, 2004, 18(4): 26)

[0046] Determine the seed production base. Choose a large-scale paddy field under large and medium-sized reservoirs in Changsha, with sufficient sunlight, few pests and diseases, fertile soil, and convenient transportation. It is required to ensure cold water supply. Within 100 meters around the planting field, there are no other rice varieties except the male parent. .

[0047] Choose the best heading and flowering period. The daily average temperature is 24-30℃, the flowering time temperature is 2...

Embodiment 3

[0058] Example 3 Seed Production of Indica Hybrid Combination You 1A / Minghui 63

[0059]Female parent: You 1A (Hybrid Rice, 1995, 10(2): 8-10). The critical temperature for the fertility conversion of You 1A, an incomplete male sterile line with nuclear-cytoplasmic interaction, is 28-29°C.

[0060] Male parent: Minghui 63, a strong restorer line of wild-type indica rice (Crop Research, 2004, 18(4): 26).

[0061] Determine the seed production base. Choose a large-scale paddy field under large and medium-sized reservoirs in Changsha, with sufficient sunlight, few pests and diseases, fertile soil, and convenient transportation. It is required to ensure cold water supply. Within 100 meters around the planting field, there are no other rice varieties except the male parent. .

[0062] Choose the best heading and flowering period. Preferably from August 10th to August 20th. Minghui 63 was sown around May 10. You 1A is generally sown 30-32 days later than Minghui 63, around June...

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Abstract

A reproductive and seed culturing method for the karyoplasmic interaction type incomplete male sterility line of rice includes such steps as choosing male parent and female parent (Karyoplasmic interaction type incomplete male sterility line), sowing, irrigating with cold water when the ambient temp is higher than the critical temp of fertility transform, hybridizing, growing and harvesting.

Description

technical field [0001] The present invention relates to the field of breeding. Specifically, the present invention relates to a breeding and seed production method of a nuclear-cytoplasmic interaction type incomplete male sterile line of rice. Background technique [0002] Rice is a self-pollinating plant, but hybrid rice has long been found to have heterosis including many desirable traits. Therefore, male sterile lines are widely valued in the field of breeding because they can avoid manual emasculation, save manpower, reduce seed costs, and ensure seed purity. Male sterile lines include nuclear male sterile lines, nucleoplasmic male sterile lines, and the like. The nuclear-cytoplasmic interaction male sterile line can be widely used in agriculture due to the following advantages: 1) it cannot produce normal pollen and can be used as a hybrid female parent; 1 can still maintain male sterility; 3) and can find a restorer line to cross with it, the produced F 1 is fertil...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01G16/00A01G22/22
Inventor 李新奇赵昌平袁隆平邓华凤
Owner HUNAN HYBRID RICE RES CENT
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