Temperature-sensitive rice male sterility line breeding method with Honglian cytoplasm

Abstract

A method for selectively culturing the light and temp sensitive male karyosterile line of rice with the red lotus type cytoplasm features the continuous backcrosses between the red lotus type sterile line as female parent and the light and temp sensitive karyosterile line carrying the red lotus type sterile line's restoring gene. If said light and temp sensitive karyosterile line does not carry the red totus type sterile line's restoring gene, said continuous backcross must be performed after the red lotus type cytoplasm restoring gene is introduced to it by hybridization or molecular marking.

Description

technical field [0001] The invention relates to the breeding and application of a rice male sterile line, in particular to a method for breeding a light-temperature-sensitive rice male sterile line with red lotus-type cytoplasm. Background technique [0002] my country is the most populous country in the world, and rice is one of the most important food crops in my country. Rice production plays a pivotal role in food security involving national stability. The "three-line" hybrid rice technology invented in the 1970s has made great contributions to increasing rice yield, and the "two-line" hybrid rice technology invented in the 1980s has further opened up broad prospects for the utilization of rice heterosis . In the application of "three-line" hybrid rice, the red lotus-type nuclear-cytoplasmic interaction male sterile line has a high outcross seed setting rate, and it is easy to obtain high yields in the reproduction of the sterile line and the production of hybrid seeds...

AI Technical Summary

Problems solved by technology

However, the red lotus-type CMS has the problem of self-fertilization at high temperature. If high temperature is encountered in the production of hybrid seeds, the CMS tends to self-fertilize, and there is a huge risk that the purity of the seed will not meet the requirements of the industry standard.

At the same time, due to the unique restorer relationship of the Honglian CMS line, there are few strong restorer lines, which limits the wide application of the CMS line.