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Culture method of dental pulp mesenchymal stem cells

A technology of mesenchymal stem cells and culture methods, applied in cell dissociation methods, tissue culture, animal cells, etc., can solve the problems of slow growth and slow adhesion of dental pulp stem cells, so as to promote healing and regeneration, arrange tightly, and delay aging Effect

Inactive Publication Date: 2018-07-13
安莱(西安)健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, the common technique is to primary culture dental pulp stem cells by enzymatic digestion, so that the cultured human dental pulp stem cells adhere slowly and grow slowly.

Method used

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  • Culture method of dental pulp mesenchymal stem cells
  • Culture method of dental pulp mesenchymal stem cells

Examples

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Embodiment 1

[0040] Such as Figure 1~2 As shown, a method for culturing dental pulp mesenchymal stem cells of the present invention comprises the following steps:

[0041]S1. Prepare the pulp sample: soak the tooth sample in buffer solution or refrigerate the tooth sample for 40h-47h, split the crown, the depth of the crown split is 0.6mm-0.9mm, and use The depth of the splitting of the crown, under aseptic conditions, exposes the pulp tissue, after which the pulp is removed. In this specific example, the tooth sample is the impacted complete third molar of a healthy adult aged 19-29, and the tooth surface needs to be sterilized before soaking and refrigerated storage. In addition, the most successful sources of dental pulp stem cells are healthy deciduous teeth and wisdom teeth. All 20 deciduous teeth can be used to extract dental pulp stem cells, and every child in the tooth replacement period is worth saving; if adults wish to have their wisdom teeth extracted, dental pulp stem cells...

Embodiment 2

[0049] A method for culturing dental pulp mesenchymal stem cells of the present invention comprises the following steps:

[0050] S1. Prepare the pulp sample: soak the tooth sample in buffer solution or refrigerate the tooth sample for 40h-47h, split the crown, the depth of the crown split is 0.6mm-0.9mm, and use The depth of the splitting of the crown, under aseptic conditions, exposes the pulp tissue, after which the pulp is removed. In this specific example, the tooth sample is the impacted complete third molar of a healthy adult aged 19-29, and the tooth surface needs to be sterilized before soaking and refrigerated storage. In addition, the most successful sources of dental pulp stem cells are healthy deciduous teeth and wisdom teeth. All 20 deciduous teeth can be used to extract dental pulp stem cells, and every child in the tooth replacement period is worth saving; if adults wish to have their wisdom teeth extracted, dental pulp stem cells can also be stored. Due to t...

Embodiment 3

[0059] Another method for culturing dental pulp mesenchymal stem cells of the present invention comprises the following steps:

[0060] S1. Prepare the pulp sample: soak the tooth sample in buffer solution or store the tooth sample in cold storage. The storage time is 40h-47h. Split the crown. The depth of the crown split is 0.6mm-0.9mm, and use The depth of the splitting of the crown, under aseptic conditions, exposes the pulp tissue, after which the pulp is removed. In this specific example, the tooth sample is the impacted complete third molar of a healthy adult aged 19-29, and the tooth surface needs to be sterilized before soaking and refrigerated storage. In addition, the most successful sources of dental pulp stem cells are healthy deciduous teeth and wisdom teeth. All 20 deciduous teeth can be used to extract dental pulp stem cells, and every child in the tooth replacement period is worth saving; if adults have the willingness to extract wisdom teeth, dental pulp stem...

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Abstract

The invention discloses a culture method of dental pulp mesenchymal stem cells. The culture method comprises the following steps: preparing a dental pulp sample, separating and culturing the dental pulp mesenchymal stem cells, carrying out colonized culture on separated dental pulp mesenchymal stem cells, detecting the dental pulp mesenchymal stem cells, and detecting and staining the dental pulpmesenchymal stem cells. The culture method of the dental pulp mesenchymal stem cells disclosed by the invention has the beneficial effects that 1, the cells cultured by being cloned and separated areclosely arranged, so that central cells are unclear in boundaries, round or irregular and cell nodules are formed, peripheral cells are polygonal or short fusiform, less in cytoplasm, large in proportion of nucleoplasm and obvious in nucleoli and some cells are fibrillar; 2, the problem of serious immunological rejection is not generated; 3, the dental pulp mesenchymal stem cells are convenient intaking materials and safe and small in cross-infection risk, thereby being capable of being used for repairing defective teeth and tooth regeneration; and 4, the dental pulp mesenchymal stem cells can promote skin wounds to heal and regenerate, delay aging or cure blindness, and further can be used for treating treat heart diseases, rheumatoid arthritis, burns, stroke or cartilage damage and thelike and is wide in use.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for culturing dental pulp mesenchymal stem cells. Background technique [0002] In 1999, "Science" rated the research results of human embryonic stem cells as the top ten scientific and technological progress in the world that year. In 2000, Time magazine listed it as the top ten scientific and technological achievements in the world at the end of the 20th century. Genome will simultaneously become the most promising field of development and application in the new century. So far, stem cell research has become one of the most striking hot spots in the fields of biology and medicine in recent years. Stem cells are primitive cells that play the role of "backbone" in the growth and development of biological individuals. They are a cell population with self-renewal, high proliferation and multi-directional differentiation potential, including embryonic stem cells and a...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0664C12N2509/00
Inventor 李杰
Owner 安莱(西安)健康产业有限公司
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