Method for induced breeding of cytoplasmic-nuclear male-sterile line by using core haploidy

Abstract

The invention relates to the field of corn hybridization, in particular to a method for induced breeding of a cytoplasmic-nuclear male-sterile line by using core haploid. The method comprises the following steps of performing hybridization by using cytoplasmic male sterility cytogene S, gene (ig) of which cytogene contains indeterminate gametophyte and an inducing line of a selection marker gene as a female parent and using an inbred line or double haploid or an excellent low-generation material of which the cytogene is N and the cell nucleus fertility recovery gene is rfrf as a male parent; screening to obtain progeny of haploid of which the cytogene is S and the cell nucleus fertility recovery gene is rf; and performing backcross on the progeny and the male parent to obtain the cytoplasmic-nuclear male-sterile line of a selfing line or double haploid of which the cytogene is S and the cell nucleus fertility recovery gene is rfrf. By the method, the improvement efficiency of the cytoplasmic-nuclear male-sterile line is improved obviously, an excellent germplasm can be quickly applied to sterilization production of hybrid seeds, purity of production of the hybrid seeds can be greatly improved, and the labor cost is saved.

Description

technical field [0001] The invention relates to the field of maize hybridization, in particular to a method for inducing nucleoplasmic interaction male sterile lines using maize haploids. Background technique [0002] Different cytoplasmic genes and different nuclear genes have different fertility, as shown in Table 1. [0003] Table 1 Fertility [0004] [0005] Obtain the hybridization results of different cytoplasmic genes and different nuclear genes such as figure 1 shown. [0006] The lineages of the different types of cytoplasmic and nuclear genes are as follows: [0007] CMS line S(rfrf), maintainer line N(rfrf), restorer line S(RfRf) or N(RfRf). [0008] The fertile offspring of F1 with normal performance can be obtained by the following methods, as follows: [0009] S(rfrf)×N(RfRf) [0010] →S(Rfrf)F1 is normal and fertile [0011] S(rfrf)×S(RfRf) [0012] The existing technology is to obtain a male sterile line similar to the parent throug...

AI Technical Summary

Problems solved by technology

[0019] (1) It takes a long time to achieve basic homozygosity of the gene locus after backcrossing for 7 generations or more;

[0020] (2) The available CMS improvement materials are relatively narrow. At present, the improvement of nuclear-cytoplasmic interaction CMS mainly uses relatively stable inbred lines as materials for backcrossing, and unstable materials are often not available;

[0021] (3) Complete homozygosity and full replacement of all sites cannot be achieved, which affects the quality of the sterile line

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