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Method for transforming hot-pepper nucleo-cytoplasmic interactive male-sterile line, homo-maintainer line and homo-restorer line

A male sterile line and maintainer technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problem that male sterile lines and isotype maintainers cannot be selected, raised, lowered or fluctuated. , dead ends of research work, etc., to achieve the effect of saving pollination labor costs, reducing production costs, and protecting intellectual property rights

Inactive Publication Date: 2011-02-16
GUIZHOU HORTICULTURAL INST (GUIZHOU HORTICULTURAL ENG TECH RES CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique allows us to combine two crops called Peppers with Bossy Brewerry Different Technologies: They have developed methods to create new crop plants without damaging their genetic material or making certain parts unusable due to lacking nutrients from another plant's genome. These techniques allow scientists to study how these crops work together well while still being able to provide useful products like foodstuffs.

Problems solved by technology

The technical problem addressed in this patented study relates to selecting specific types of female parents based upon their own characteristics such as size, color pattern, shape, etc., which can lead to inferences about whether these individuals develop true sexual maturities due to lack of efficient ways to distinguish between different forms of reproductive interference without compromising yield rates.

Method used

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Examples

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Effect test

Embodiment 1

[0034] Embodiment 1 of the present invention: the method of transferring the male sterile line, the homotype maintainer line and the homotype restorer line of capsicum, in Sanya in the winter of 2007, Chaotian Xiaojiaojiao PBC521 was used as the male parent to interact with the introduced capsicum nucleoplasm For male sterile line hybridization, the male parent plants are self-fertilized, and the seeds are harvested separately; in spring and summer of 2008 in Guiyang, the physical separation of the hybrid progeny group was observed, and the male parent progeny was selected as a pair with the sterile plants of the hybrid progeny Backcrossing, the male parent plants are self-crossed, and the seeds are harvested separately; in the winter of 2008, the two backcross progeny showed group physical separation again; The male strains were backcrossed in pairs, the male parent strains were self-crossed, and the seeds were harvested separately; the fertility status of the backcross offspr...

Embodiment 2

[0035] Example 2 of the present invention: the method of transferring male sterile lines, isotype maintainers and isotype restorers of capsicum, in Sanya in the winter of 2007, Chaotian pepper was ideally single as the male parent and introduced capsicum nucleoplasm interaction The male sterile line is crossed, the male parent plants are selfed, and the seeds are harvested separately; in the spring and summer of 2008 in Guiyang, it was observed that the population of the hybrid offspring was sterile, and two single plants of the male parent and offspring were selected to pair with the sterile plants of the hybrid offspring Backcross, the male parent plants are self-crossed, and the seeds are harvested separately; in the winter of 2008, the two backcross progeny showed group physical separation again; The male strains were backcrossed in pairs, the male parent strains were self-crossed, and the seeds were harvested separately; the fertility status of the backcrossed offspring ob...

Embodiment 3

[0036] Example 3 of the present invention: the method of transferring male sterile lines, isotype maintainer lines and isotype restorer lines of pepper. In the spring and summer of 2009, the pepper inbred line X-1 was used as the male parent and the sterile line Y92A hybridization, the male parent strains were self-crossed, and the seeds were harvested separately; in the winter of 2009, it was observed that the population of the hybrid offspring was fertilely segregated, and 50 individual plants from the male parent were selected for paired backcrossing with the inflorescences of the sterile strains of the hybrid offspring. Self-crossing and harvesting separately; the fertility status of the backcross offspring observed in the spring and summer of 2010: 5 backcross offspring are all sterile, and the corresponding male parent is a single plant of the maintainer line, and one of them is selected as a continuous pair The male sterile line X-1A and the isotype maintainer line X-1B ...

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Abstract

The invention discloses a method for transforming a hot-pepper nucleo-cytoplasmic interactive male-sterile line, a homo-maintainer line and a homo-restorer line, which comprises the following steps of: hybridizing by using a hot-pepper selfing line as a male parent and the hot-pepper nucleo-cytoplasmic interactive male-sterile line as a female parent, simultaneously selfing a male parent plant, selecting sterile plants or other inflorescences in 4-50 corresponding male-parent filial single plants and hot-pepper filial generations for paired test crossing when in filial-generation fertility separation, selfing each male-parent single plant, and respectively processing according to the group fertility expression of test crossing generations to select the male-sterile line, the homo-maintainer line and the homo-restorer line. When in filial-generation group fertility separation, the invention not only can be used for transforming the hot-pepper nucleo-cytoplasmic interactive male-sterile line and the homo-maintainer line from the hot-pepper selfing line, but also can be used for breeding the homo-restorer line, expands the selecting space of the restored male parents of other homologous sterile lines and can be used for screening more excellent three-line combined varieties. A hot-pepper hybrid is produced by adopting a male-sterile three-line technology, all the complex pollination procedures are simplified, and the economic benefit is quite remarkable.

Description

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Claims

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Application Information

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Owner GUIZHOU HORTICULTURAL INST (GUIZHOU HORTICULTURAL ENG TECH RES CENT)
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