Method for rapidly breeding nucleo-cytoplasmic interaction sterile lines

Abstract

The invention belongs to the technical field of agricultural seed breeding and discloses a method for rapidly breeding nucleo-cytoplasmic interaction sterile lines. The method comprises the following steps of: utilizing the existing male sterile lines and a plurality of homozygous and excellent selfing line male parent materials to carry out paired testcross; carrying out simultaneous selfing on male parents, and analyzing offspring fertility; selecting 12 sterile plants in a fertility-separation testcross combination for one-to-one backcross with male-parent selfing offspring; and rapidly transbreeding out male sterile lines and maintainer lines in a third-generation backcross combination; and the method comprises the detailed steps of: screening a first generation, screening a second generation and screening a third generation. The method disclosed by the invention has the advantages that excellent selfing lines can be rapidly transbreeded into the male sterile lines, the maintainer lines are screened out, and the sterile degree reaches 100%; 12 plants are selected in the male-parent selfing offspring to carry out testcross with 12 sterile plants in F1 generation for three times repeatedly, so that the probability of obtaining the sterile lines and the maintainer lines is increased.

Description

technical field [0001] The invention belongs to the technical field of agricultural seed breeding, and in particular relates to a method for rapidly selecting a nuclear-cytoplasmic interaction sterile line. Background technique [0002] Capsicum, as a vegetable crop with frequent cross-pollination, has obvious heterosis. The first generation of excellent hybrids can increase the yield by more than 50% compared with the general production species. It has been widely used in production and achieved obvious economic benefits. Pepper hybrid seed production is often artificially detasselled at the bud stage, which is labor-intensive and costly, and the purity of the seeds is difficult to guarantee. The use of pepper male sterile lines to produce hybrid varieties is an effective means to greatly reduce the cost of hybrid seed production and ensure the purity of seeds. When breeding pepper male sterile lines, the first task is to transfer the excellent inbred lines into sterile lin...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, 1-2 paternal offspring are selected for backcrossing with sterile plants each time, and the selection population is small, and the fertility of the offspring of multiple backcrosses is unstable, and the situation of complete sterility is difficult to occur, and even more The fertility of the backcross offspring can be fully restored for the first time, which is a bottleneck for the research on male sterile lines and maintainer lines from excellent inbred lines, which will lead to the inability to breed male sterile lines and maintainer lines from many excellent inbred line materials. Tie

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