Molecule marker of characteristics of cotton yield or quality of fibre
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A cotton fiber and molecular marker technology, which can be used in plant genetic improvement, botanical equipment and methods, applications, etc., and can solve problems such as low efficiency
Inactive Publication Date: 2006-07-05
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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[0014] Selection is one of the most important links in breeding. Traditional phenotype-based selection methods have many shortcomings and low efficiency. Molecular markers p
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Embodiment 1
[0044] Extraction and Purification of Cotton Genomic DNA
[0045] Referring to the method reported by Song Guoli et al. (1998) with slight modifications, the basic steps are briefly described as follows:
[0046] (1) Cotton genomic DNA extraction:
[0047] Step 1: Add 2% β-mercaptoethanol to the extract, and preheat it in water at 65°C.
[0048] Step 2: Take out an appropriate amount of frozen leaves (about 2g) from the ultra-low temperature (-70°C) refrigerator, immediately put them into a pre-cooled mortar, add liquid nitrogen, quickly grind them into powder, and immediately put them into a container with a small amount of activated carbon. Add 0.6ml extract solution to a 1.5ml centrifuge tube, mix well, and bathe in water at 65°C for 60min. (Mix once every 10 minutes or so to fully disperse the sample).
[0049] The third step: after the water bath is completed, add an equal volume of chloroform-isoamyl alcohol (24:1, V / V), cover the lid, slowly invert the centrifuge tub...
Embodiment 2
[0062] Establishment of target traits pole plant bank
[0063] According to the BSA method proposed by Michelmore (1991), 5 F2 individual plants with extreme performance of each trait were selected from the population, and their DNA was mixed in equal amounts to construct the extreme value gene pool of each trait, with a final concentration of 10 ng / μl .
Embodiment 3
[0065] Tag screening
[0066] According to the performance of fiber traits in the F2 population, a mixed pool of extreme value DNA for six traits, including fiber strength, length, micronaire value, uniformity, coat percentage, and elongation, was constructed, and the parents were first screened with 386 pairs of SSR primers. The differential primers were then screened with the established extreme DNA pool, and the differential primers obtained from the extreme DNA pool were amplified and detected for all F2 single plants, and a total of 5 polymorphic markers were obtained.
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Abstract
This invention relates to a molecule marking method of selecting cotton yields and properties or fiber qualities using SSR technology, which includes: a) cross-fertilization of Sino-G97038 cotton group with the 16th group from China Institute of Cotton (Sino-16), with its F1 generation selfing to produce the F2 generation; b) extraction of the whole DNA in the leaves of F2 (Sino-G97038í‡Sino-16) separate group by means of CTAB; c) establishment of target character extremum bank by means of BSA; d) selection of parent species with SSR primers, further selection of discrepantly behaving primers between parent species with established extremum DNA bank, and amplification of the obtained discrepant primers and all F2 individual plants so as to gain polymorphism markers; e) SSR amplification and detection; f) data analysis. This invention publishes the molecular markers of the cotton fiber qualities, which have a nucleotide sequence from SEQ ID No.1 to SEQ ID No.4. Besides, it also publishes the application of the molecular markers of cotton yield and properties and fiber qualities in seed breeding.
Description
field of invention [0001] The invention relates to a molecular marker of cotton yield character or fiber quality. Specifically, the present invention relates to a method for screening cotton yield traits or fiber quality molecular markers using SSR markers and BSA methods, and discloses the sequence of the molecular markers. In addition, the invention discloses the application of the molecular marker in cotton breeding. Background technique [0002] Cotton is an important economic crop in my country and plays an extremely important role in the national economy and people's lives. Cotton fiber is the main raw material of the textile industry. It has the characteristics of heat preservation, heat insulation, moisture absorption, breathability, comfort and easy dyeing, and has been favored since ancient times. In the modern social life of advocating nature and pursuing comfort, cotton fiber has attracted more attention, and its various advantages cannot be replaced by chemica...
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