Preparation method of rice genetic engineering sterile line

Abstract

The invention discloses a preparation method of a rice genetic engineering sterile line. The preparation method comprises the following steps: by using the Afl2 restriction Enzyme cutting sites introduced to the upstream and downstream, connecting the corn lethal gene ZMAA1, through a single restriction Enzyme cutting site, to a pCAMBIA1300 binary vector already connected with a DsRED gene expression element and the rice EAT1 gene. The restriction enzyme cutting sites Kpn I and Sma I introduced into the upstream and downstream primers and the restriction enzyme cutting sites Kpn I and Sma I onthe pCAMBIA1300 binary vector are utilized. According to the method disclosed by the invention, the F1-generation hybrid conforms to the Mendel's law of segregation in the self-seed process, and theoffsprings include the hybrids capable of maintaining the triple-linked genes, as well as the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

However, the rice EAT1 gene mutation will cause the tapetum cells to fail to degrade in time, and the programmed death will be delayed, so the callose enzyme secreted by the tapetum will affect the development and separation of microspores, and finally show sterility

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