Preparation method of rice genetic engineering sterile line

A genetic engineering and rice technology, applied in the field of molecular biology and nucleic acid chemistry, can solve problems such as sterility, affecting the development and separation of microspores, and delaying programmed death

Inactive Publication Date: 2018-07-03
QINGDAO YUANCE GRP CO LTD
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low
However, the rice EAT1 gene mutation will cause the t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of rice genetic engineering sterile line
  • Preparation method of rice genetic engineering sterile line
  • Preparation method of rice genetic engineering sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] The present invention is described in detail below in conjunction with embodiment. In order to make the purpose, technical solution and advantages of the present invention clearer and clearer, the present invention will be described in further detail below with reference to the accompanying drawings and examples, but the present invention is not limited to these examples.

[0040] In the invention, the common nuclear sterile gene EAT1 of rice is linked with the lethal gene ZMAA1 of maize, and the red fluorescent protein gene DsRED is used as a reporter gene to carry out genetic modification on the rice eatl mutant to obtain F1 generation hybrids. The hybrids of the F1 generation follow Mendel's segregation law in the process of self-fertilization, and the offspring produced include heterozygotes that can maintain the triple-linked genes and sterile sterile lines, the so-called third-generation sterile lines.

[0041] In order to solve the above technical problems, the i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a rice genetic engineering sterile line. The preparation method comprises the following steps: by using the Afl2 restriction Enzyme cutting sites introduced to the upstream and downstream, connecting the corn lethal gene ZMAA1, through a single restriction Enzyme cutting site, to a pCAMBIA1300 binary vector already connected with a DsRED gene expression element and the rice EAT1 gene. The restriction enzyme cutting sites Kpn I and Sma I introduced into the upstream and downstream primers and the restriction enzyme cutting sites Kpn I and Sma I onthe pCAMBIA1300 binary vector are utilized. According to the method disclosed by the invention, the F1-generation hybrid conforms to the Mendel's law of segregation in the self-seed process, and theoffsprings include the hybrids capable of maintaining the triple-linked genes, as well as the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/82C12N15/65A01H5/00A01H6/46
CPCC07K14/415C12N15/65C12N15/8287
Inventor 张国栋刘佳音罗碧葛旭娟徐春莹米铁柱
Owner QINGDAO YUANCE GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap