Method for combining hybrid bibasic seed with cytoplasmic male sterility system

Abstract

The invention relates to a method for combining the first generation of hybrid seeds by kenaf cytoplasmic male sterile line, and belongs to the technical field of combination of crop hybrid seeds. The method is characterized in that the cytoplasmic male sterile line (also called cytoplasmic-nuclear male sterile line) taken as female parent and restoring line taken as male parent are combined into the first generation of the hybrid seeds which breed the second generation of the hybrid seeds through inbred breeding. The method is novel due to that the separation of male sterile plants and fertile plants exists in the second generation of the hybrid seeds, but kenaf takes the harvest of stalks or bast fibre as breeding aim and does not need seeds, so the male sterile plants have no adverse affection to the yield of the bast fibre. The method saves the labor input of kenaf chemical hybridizing and artificial pollination, and can guarantee the purity of the hybrid seeds because of stable fertility of the kenaf cytoplasmic male sterile line.

Description

Technical field:

[0001] The invention relates to a method for combining crop hybrid seeds, in particular to a second-generation hybrid (F 2 generation) seed method. Background technique:

[0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stems, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of kenaf breeding at home and abroad.

[0003] In 1994, Tang Yonghai and Li Defang of the Hemp Research Institute of the Chinese Academy of Agricultural Sciences invented "a method of kenaf hybrid seed production" (application number: CN94103929.3) after years of research. The pollination method prepares the first generation of hybrids, the stamen abortion rate is about 95%, the purity of the hybrids is above 90%, and the first generation of hybrids self-propagates the second generation of hybrids. [000...

Examples

Embodiment 1

[0011] Kenaf cytoplasmic male sterile K03A (see invention patent: ZL200510019454.4) was used as the female parent, and K03B (the maintainer line of K03A) was used as the male parent to reproduce the cytoplasmic male sterile line K03A; the kenaf cytoplasmic male sterile line K03A was used as the parent. As the female parent, Fuhong 992 (provided by Fujian Agriculture and Forestry University) was used as the male parent to produce the kenaf hybrid K03-2, which is normally fertile, high-yielding, high-quality, and highly resistant to kenaf anthracnose. This variety passed the expert appraisal organized by the National Agricultural Technology Extension Service Center in 2007, and was named "Hongyou No. 1" (Guopinjianma 2007003). The second-generation hybrid seeds of "Hongyou 1" can be obtained by self-propagation of "Hongyou 1".

Technical field:

[0001] The invention relates to a method for combining crop hybrid seeds, in particular to a second-generation hybrid (F 2 generation) seed method. Background technique:

[0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stems, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of kenaf breeding at home and abroad.

[0003] In 1994, Tang Yonghai and Li Defang of the Hemp Research Institute of the Chinese Academy of Agricultural Sciences invented "a method of kenaf hybrid seed production" (application number: CN94103929.3) after years of research. The pollination method prepares the first generation of hybrids, the stamen abortion rate is about 95%, the purity of the hybrids is above 90%, and the first generation of hybrids self-propagates the second generation of hybrids. [000...

Examples

Embodiment 1

[0011] Kenaf cytoplasmic male sterile K03A (see invention patent: ZL200510019454.4) was used as the female parent, and K03B (the maintainer line of K03A) was used as the male parent to reproduce the cytoplasmic male sterile line K03A; the kenaf cytoplasmic male sterile line K03A was used as the parent. As the female parent, Fuhong 992 (provided by Fujian Agriculture and Forestry University) was used as the male parent to produce the kenaf hybrid K03-2, which is normally fertile, high-yielding, high-quality, and highly resistant to kenaf anthracnose. This variety passed the expert appraisal organized by the National Agricultural Technology Extension Service Center in 2007, and was named "Hongyou No. 1" (Guopinjianma 2007003). The second-generation hybrid seeds of "Hongyou 1" can be obtained by self-propagation of "Hongyou 1".