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Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof

A technology of Brassica napus and restorer lines, which is applied in the field of research on the utilization of heterosis in rapeseed, can solve the problem of no obvious progress in the selection of homozygous Brassica napus OguCMS restoration materials, and achieves overcoming reproductive barriers, with obvious effects and simple operation Effect

Inactive Publication Date: 2012-08-22
湖南省作物研究所
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] China introduced Brassica napus Ogu CMS in the late 1970s. In terms of restoration materials, Li Xufeng (2001) introduced the restorer gene from the radish variety Lanhuazi into Brassica napus Ogu CMS, and obtained the additional chromosome restoration material Ad -6. There has been no significant progress in the screening of homozygous Brassica napus Ogu CMS recovery materials

Method used

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  • Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof
  • Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof
  • Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof

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Embodiment

[0031] a kind of like Figure 9 , Figure 10 The Brassica napus Ogu CMS restorer line CLR650 of the present invention is shown, the preservation number of the Brassica napus Ogu CMS restorer line in the General Microbiology Center of China Microorganism Culture Collection Management Committee is CGMCCNo.6003, and its plant name is Ogu CMS restorer line CLR650 .

[0032] The method of transfection of the Brassica napus Ogu CMS restorer line CLR650 of the present embodiment is as follows: Figure 11 As shown, it specifically includes the following steps.

[0033] 1. Introduction of exogenous radish restoration gene

[0034] 1.1 Materials

[0035] Mainly include: such as figure 2 The shown radish-blue (Raphanobrassica) 94-1 (AACCRR, 2n=58) was donated by Academician Fu Tingdong of Huazhong Agricultural University in 1993 (genetic background unknown); image 3 The shown Brassica napus Ogu CMS (lower leaf color) chlorogenic sterile line H13A (Brassic Napus.L, 2N=38) was intr...

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Abstract

The invention discloses a cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and an obtained derivative restorer material after the cabbage type rape Ogu CMS restorer is transferred, wherein the preservation number of the Chinese microbial culture preservation management committee center for general microbiology is CGMCC No. 6001, and the plant name is Ogu CMS restorer CLR650. A transformation method of the cabbage type rape OgU CMS restorer comprises the following steps: using a cruciferae resource containing Ogu CMS exogenous restoring genes as a restoring gene donor parent, utilizing a grafting technology to overcome distant hybridization reproductive dysfunction, leading intergeneric radish restoring genes into cabbage type rapes, continuously culturing fertile plants with chlorosis, and obtaining the restorer CLR650 which can be fertile in selfing and test-crossing after screening, selfing and backcross screening for multiple generations. The restorer material is low in cost, good in safety and capable of being applied to a cabbage type rape Ogu CMS pollination system.

Description

technical field [0001] The invention belongs to the research field of heterosis utilization of rapeseed, and in particular relates to a recovery material of Brassica napus and its transgenic method and application. Background technique [0002] The radish cytoplasmic male sterility (Ogu CMS) created by Ogura (1968) has the remarkable characteristics of stable sterility and easy maintenance, and is recognized as the most ideal way to utilize heterosis in the whole brassica crop. In the application research of Brassica napus, Bannerot (1974) was the first to breed Brassica napus Ogu CMS by backcrossing method. This sterile type has undesirable characteristics such as low temperature and chlorosis in leaf color and lack of nectaries due to inconsistency of plasmonucleus. Pelletier (1983) carried out protoplast fusion of Brassica napus and radish, and bred a new generation of Ogu-INRA CMS, which not only maintained the original sterility stability, but also overcome the problems...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 陈卫江
Owner 湖南省作物研究所
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