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Onion cytoplasmic male sterility SCAR mark and uses thereof

A male sterility and cytoplasmic technology, applied in the biological field, can solve the problems of poor accuracy and stability of RAPD labeling, cumbersome operation, unsuitability, etc., and achieve the effect of completely consistent identification results, simple operation process and cost saving

Inactive Publication Date: 2009-07-29
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

RFLP markers and AFLP markers are relatively cumbersome to operate, while RAPD markers have poor accuracy and stability, and are not suitable for large-scale identification of field materials; SCAR markers are easy to operate, high in accuracy, and high in stability. Disadvantages, becoming the molecular labeling method of choice

Method used

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  • Onion cytoplasmic male sterility SCAR mark and uses thereof
  • Onion cytoplasmic male sterility SCAR mark and uses thereof
  • Onion cytoplasmic male sterility SCAR mark and uses thereof

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Embodiment 1

[0035] (1) Materials and methods

[0036] 1 DNA extraction and detection

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Abstract

The invention discloses an onion cytoplasm male sterility SCAR marker, wherein the length of the specific fragment of the SCAR marker is 339bp, and the nucleotide sequence thereof is shown as SEQ ID No.2. The invention further discloses a primer of the SCAR maker, wherein the forward primer is Zt339-u:5'-GTTCTCAAGCAGATTCCGCAC-3', and the reverse primer is Zt339-d:5'-AGGACCAAACCGAGAGTGAGC-3'. Quick and accurate selection for onion cytoplasm fertility can be realized by using the obtained SCAR marker, thereby quickening the seed selection for new breed strain of onion cytoplasm male sterility, and establishing novel onion coenospecies parent selection system; first-filial generation breeds with independent intellectual property are selected and bred, thereby solving the difficult problems that conventional seeds are mainly used in the current production in China and large sums of foreign exchange is used for buying foreign coenospecies.

Description

Technical field [0001] The invention relates to a SCAR marker and its application, in particular to an onion cytoplasmic male sterility SCAR marker and its application, and belongs to the field of biotechnology. Background technique [0002] Onion (Allium cepa.L), also known as shallot, shallot, Liliaceae, Allium genus, is a biennial plant. It has a cultivation history of more than 5,000 years. It is a major vegetable species in the world and is known as a health food. Cultivated varieties and export varieties [Hou XL, Wu ZX. Cultivation, storage and processing of onion in China. In: the international symposyium on the utilization and processing of onions, 1997, 11:107-121]. According to preliminary statistics, my country’s onion planting area has reached 226,000 ha 2 (1999), ranking second in the world, with a total output of 4.629 million tons, ranking first in the world [Su Baole. Foreign exchange earning vegetable export guide. Beijing: China Agricultural Science and Technolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 吴雄修景润杨妍妍刘冰江霍雨猛张一卉陈运起徐培文缪军孔素萍高莉敏朴玄春廉美兰吴松权朴钟云权太勇刘栓桃李巧云徐鑫霍凤梅
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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