Series molecular markers closely linked with muskmelon powdery mildew resistant gene Pm-2Fand acquisition method of series molecular markers

A powdery mildew resistance gene and molecular marker technology, applied in the field of genetic engineering, can solve the problem of the long genetic distance between the marker and the target gene

Inactive Publication Date: 2015-05-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the small population used for marker linkage analysis and the relatively long genetic distance between the marker and the targe

Method used

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  • Series molecular markers closely linked with muskmelon powdery mildew resistant gene Pm-2Fand acquisition method of series molecular markers
  • Series molecular markers closely linked with muskmelon powdery mildew resistant gene Pm-2Fand acquisition method of series molecular markers
  • Series molecular markers closely linked with muskmelon powdery mildew resistant gene Pm-2Fand acquisition method of series molecular markers

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Embodiment Construction

[0096] Specific embodiments of the present invention will be described in detail below.

[0097] According to the first aspect of the present invention, the present invention provides a series of CAPS / Indel markers closely linked to the melon powdery mildew resistance gene Pm-2F, which has at least one nucleotide base in SEQ ID NO: 1-8 in the sequence listing base sequence.

[0098] in,

[0099] In the sequence listing, SEQ ID NO:1 is the nucleotide base sequence specifically amplified by the primer CAPS-Dde I against disease materials;

[0100] In the sequence listing, SEQ ID NO: 2 is the nucleotide base sequence of the primer CAPS-Dde I for the specific amplification of the susceptible material;

[0101] In the sequence listing, SEQ ID NO:3 is the nucleotide base sequence of the primer CAPS-Hph I specifically amplified by the disease-resistant material;

[0102] In the sequence listing, SEQ ID NO:4 is the nucleotide base sequence of the primer CAPS-Hph I for the specific ...

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Abstract

The invention belongs to the field of genetic engineering and provides series molecular markers closely linked with a muskmelon powdery mildew resistant gene Pm-2F and an acquisition method of the series molecular markers. Each molecular marker contains at least one nucleotide base sequences shown in SEQ No:1-8 in a sequence table. The acquisition method of the series molecular markers comprises the following steps: selecting a test material, primarily positioning the muskmelon powdery mildew resistant gene Pm-2F, acquiring a site SNP/Indel linked with target characters, and acquiring molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F, so that the molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F are obtained. When the molecular markers are used for early screening and identification of a powdery mildew resistant variety, the breeding period can be greatly shortened, and the breeding efficiency is improved, so that the series molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F have important theory and practice significance.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a series of molecular markers closely linked with the melon powdery mildew resistance gene Pm-2F and an acquisition method thereof. Background technique [0002] Melon (Cucumis melo L.) is an important fruit-type vegetable crop of the genus Cucurbitaceae. It is widely cultivated at home and abroad. Melon is a dominant crop in high-end agriculture in my country, which is of great significance to increasing farmers' income and adjusting agricultural structure. Powdery mildew is a worldwide disease that seriously endangers melon production, and it can occur from seedling stage to harvest stage. Powdery mildew mainly harms the leaves of muskmelon, and also infects petioles and vines, which can lead to a decrease in plant photosynthetic capacity, premature aging and even death, seriously affecting the yield and quality of muskmelon. In recent years, with the scale of pr...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10
Inventor 许勇张春秋张海英宫国义郭绍贵任毅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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