Series molecular markers closely linked with muskmelon powdery mildew resistant gene Pm-2Fand acquisition method of series molecular markers

Abstract

The invention belongs to the field of genetic engineering and provides series molecular markers closely linked with a muskmelon powdery mildew resistant gene Pm-2F and an acquisition method of the series molecular markers. Each molecular marker contains at least one nucleotide base sequences shown in SEQ No:1-8 in a sequence table. The acquisition method of the series molecular markers comprises the following steps: selecting a test material, primarily positioning the muskmelon powdery mildew resistant gene Pm-2F, acquiring a site SNP / Indel linked with target characters, and acquiring molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F, so that the molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F are obtained. When the molecular markers are used for early screening and identification of a powdery mildew resistant variety, the breeding period can be greatly shortened, and the breeding efficiency is improved, so that the series molecular markers closely linked with the muskmelon powdery mildew resistant gene Pm-2F have important theory and practice significance.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a series of molecular markers closely linked with the melon powdery mildew resistance gene Pm-2F and an acquisition method thereof. Background technique [0002] Melon (Cucumis melo L.) is an important fruit-type vegetable crop of the genus Cucurbitaceae. It is widely cultivated at home and abroad. Melon is a dominant crop in high-end agriculture in my country, which is of great significance to increasing farmers' income and adjusting agricultural structure. Powdery mildew is a worldwide disease that seriously endangers melon production, and it can occur from seedling stage to harvest stage. Powdery mildew mainly harms the leaves of muskmelon, and also infects petioles and vines, which can lead to a decrease in plant photosynthetic capacity, premature aging and even death, seriously affecting the yield and quality of muskmelon. In recent years, with the scale of pr...

AI Technical Summary

Problems solved by technology

However, due to the small population used for marker linkage analysis and the relatively long genetic distance between the marker and the target gene, there are still some limitations in the application of these two markers to assisted breeding for resistance to powdery mildew in melon.

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