Molecular detection method of Brassica napus self-incompatible S-locus haplotype

A Brassica napus and haplotype technology, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of low hybrid combining ability, select and breed self-incompatible restorer lines Efficiency, low and other issues

Inactive Publication Date: 2013-12-18
HUAZHONG AGRI UNIV
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  • Abstract
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Problems solved by technology

Create a molecular detection technology for the S haplotype of Brassica napus, which can be used to accurately identify self-incompatibility, and can solve the problem of low efficiency in breedi

Method used

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  • Molecular detection method of Brassica napus self-incompatible S-locus haplotype
  • Molecular detection method of Brassica napus self-incompatible S-locus haplotype
  • Molecular detection method of Brassica napus self-incompatible S-locus haplotype

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Embodiment 1

[0057] 1. Acquisition of segregated groups

[0058] Brassica napus self-incompatibility line S-1300 (see literature: Ma Chaozhi et al., Breeding of Brassica napus double-low self-incompatibility line, Journal of Huazhong Agricultural University, 1998, 17 (3): 211-213 ) as the female parent and the Brassica napus self-incompatibility restorer line '8400' as the male parent (Gao et al. The genetic characterization of self-incompatibility in a Brassica napus line with promising breeding potential, Mol Breed2013, 31: 485-493) get F 1 seed, the F 1 Seeds are sown to get F 1 plant, the plant was bagged and selfed to obtain F 2 separate groups, take F 1 Plant pollen was backcrossed with S-1300 to obtain BC 1 Separate groups. Similarly, the female parent of Brassica napus self-incompatibility line S-1300 and the male parent of Brassica napus self-incompatibility maintainer line Bing409 were crossed to obtain F 1 seed, the F 1 Seeds are sown to get F 1 plant, the plant was bag...

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Abstract

The invention belongs to the field of molecular breeding of rapeseeds, and relates to a molecular detection method of Brassica napus self-incompatible S-locus haplotype, a molecular marker and application. An SCAR (sequence characterized amplified regions) molecular marker system used for identifying the Brassica napus self-incompatible S-locus haplotype is prepared and includes four SCAR molecular markers, namely, SRK1-1, SCR1-1, SRK-1300 and SCR7-2 which can be combined to obtain two pairs of double PCR (polymerase chain reaction) markers as SRK1-1 and SRK-1300 as well as SCR7-2 and SRK-1300; the PCR marker combination can be used in a combination manner or independent manner; the DNA (deoxyribonucleic acid) sequences of the PCR marker combination are shown as SEQ ID NO: 6, SEQ ID NO:7, SEQ ID NO: 8 and SEQ ID NO: 9. According to the invention, a practical marker and an utilization method are provided for the molecular breeding of the rapeseeds.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a molecular detection method and application of S haplotype of self-incompatibility site in Brassica napus. Background technique [0002] Using heterosis to increase yield is an important goal and development direction of domestic and foreign rapeseed production. The methods of utilizing heterosis in rapeseed include cytoplasmic male sterility, nuclear male sterility, self-incompatibility, chemical male sterility and so on. Cytoplasmic male sterility is currently the most widely used way to utilize heterosis in rapeseed, although it has disadvantages such as long breeding cycle for maintainer lines and restorer lines, and potential negative cytoplasmic effects. In my country, the cytoplasmic male sterile polyma is mainly used, and the hybrids bred by it occupy about 60% of our rape planting area; in foreign countries, the cytoplasmic male sterile radish is ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 马朝芝高长斌翟文周贵龙张彤
Owner HUAZHONG AGRI UNIV
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