Method for identifying pathogen of paroxysmal foliage wilting disease in myrica rubra

Abstract

The invention provides a method for identifying pathogen of the paroxysmal foliage wilting disease in myrica rubra, comprising separating the xylem from the phloem of a myrica rubra branch, respectively cutting the phloem and xylem into small sheets of tissue without the surface disinfection, placing them on a PDA flat, culturing in a thermotank of 25 DEG C for 2 days, picking out fluffy fungal hyphae and placing the fungal hyphae to another new PDA flat, continuously culturing in the thermotank of 25 DEG C for 5 days, and picking out purified hypha blocks of 0.5cm<2> for the inoculation experiment of the pathogenicity. The method for testing the pathogen of the paroxysmal foliage wilting disease in myrica rubra in vitro is reliable and fast, needs no expensive materials, comprises simplesteps, and has high accuracy.

Description

technical field [0001] The invention belongs to the field of plant disease identification, and in particular relates to an identification method for sudden leaf wilt of bayberry. Background technique [0002] Myrica rubra (Myrica rubra) is a unique fruit in southern my country. The fruit is sweet and sour and has a unique flavor. It enjoys a high reputation at home and abroad. In 2008, the Yangmei cultivation area in our province was nearly 1.2 million mu, with an output of more than 350,000 tons and a total output value of more than 2 billion yuan. When the bayberry industry is playing an increasingly important role in poverty alleviation for farmers in mountainous areas, a new disease, sudden wilt of bayberry branches and leaves, has occurred in Xianju, Ruian, Linhai, Huangyan and other places in our province in recent years. According to complete statistics, the area of ​​Xianju alone that is on the verge of dying has reached more than 5,000 mu. It can be seen that the s...

AI Technical Summary

Problems solved by technology

When the bayberry industry is playing an increasingly important role in poverty alleviation for farmers in mountainous areas, a new disease, sudden wilt of bayberry branches and leaves, has occurred in Xianju, Ruian, Linhai, Huangyan and other places in our province in recent years. According to complete statistics, the area of ​​Xianju alone that is on the verge of dying has r...

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