Composition containing trichoderma and mortierella and application of composition in biological prevention and control of panax notoginseng

Abstract

The invention relates to the technical field of plant disease prevention and control, in particular to a composition containing trichoderma and mortierella as well as a preparation and application thereof. The composition comprises bacterial strains, conidia and / or volatile matters of bacterial cultures of Trichoderma harzianum (Trichoderma harzianum) 3NG-1 and Mortierella alpina (Mortierella alpina) B28-1, wherein the volume ratio of the Trichoderma harzianum (Trichoderma harzianum) 3NG-1 to the Mortierella alpina (Mortierella alpina (Mortierella alpina) B28-1 is (20: 1) to (1: 20). The composition and the preparation thereof disclosed by the invention are used for biological prevention and treatment of pseudo-ginseng diseases, relieving soil continuous cropping effect in the growth process of pseudo-ginseng and promoting the growth of pseudo-ginseng. The method has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of plant disease prevention and control, and in particular to a composition comprising Trichoderma and Mortierella and application thereof in biological control of Panax notoginseng. Background Art

[0002] Panax notoginseng is a traditional precious Chinese medicinal material. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain. It also has significant therapeutic effects on cardiovascular and cerebrovascular diseases. It has high medicinal and economic value, and people's demand for Panax notoginseng is increasing day by day. In order to meet market demand, Panax notoginseng has been planted on a large scale for many years. Due to its unique growth habits, it is easy to cause pathogens to accumulate and diseases to break out. Among them, soil-borne root rot is particularly serious, causing Panax notoginseng to wither and die, and aggravating the obstacles of continuous cropping.

[0003] The imbalance of soil microbial community structure, the decrease in the abundance of beneficial microorganisms, and the large accumulation of soil-borne pathogens are the main causes of Panax notoginseng root rot. At present, the methods for preventing and controlling Panax notoginseng root rot include crop rotation, chemical control, soil sterilization, etc., but they all have certain disadvantages. For example, the long cycle required for crop rotation makes it difficult to maintain the sustainable development of the Panax notoginseng industry; chemical control may cause problems such as pesticide residues and excessive heavy metals; soil sterilization requires high costs and will kill indigenous beneficial microorganisms in the soil. Biological control is an environmentally friendly method that uses beneficial microorganisms to regulate the composition of soil microorganisms to prevent and control plant diseases. Many beneficial microorganisms have been used as biofertilizers or microbial preparations in agricultural production.

[0004] As an important biocontrol fungus, Trichoderma has the advantages of broad spectrum and high efficiency. It has certain control effects on many kinds of plant diseases, such as Fusarium oxysporum, Rhizoctonia solani, Pythium ultimum, Pseudomonas syringae, Xanthomonas, etc. Trichoderma has a variety of biological control mechanisms, including competition, parasitism, induced resistance and antibiotics. While inhibiting pathogens, Trichoderma can also promote plant growth, improve plant stress resistance, root nutrient utilization and regulate soil ecological balance. However, the effect of using a single Trichoderma to control crop diseases is sometimes not ideal, so the synergistic effect of Trichoderma with other biological or non-biological factors in disease prevention has received more and more attention.

[0005] As a dominant genus, Mortierella is widely present in the rhizosphere microbial community of some healthy plants. It can produce auxin (IAA), siderophore, and increase the content of available nitrogen and available phosphorus in the rhizosphere soil to promote plant growth. In addition, studies have reported that Mortierella can regulate soil microbial communities and significantly alleviate ginseng root rot caused by Fusarium oxysporum. The arachidonic acid produced by it can act as a signal substance to activate plant defense pathways and reduce the occurrence of diseases. Therefore, Mortierella has great potential in improving plant nutrient absorption efficiency, protecting crops from adverse conditions, and resisting pathogens.

[0006] In the past decade, multi-omics sequencing technology has been widely used in plant microbiome research and revealed that it is the complex microbial community combination in the rhizosphere rather than a single microbial strain that protects plants from pathogen infection. Studies have shown that the mixed action of non-antagonistic biocontrol agents can overcome the shortcomings of single agents in the field, such as insignificant effect, low broad-spectrum antibacterial activity, and inability to exert maximum advantages. In order to solve these problems, multiple strains with distant kinship and non-antagonistic properties are combined and applied to the field. Multiple bacteria play their own biocontrol roles, and each strain coordinates and works together to prevent and control plant diseases, effectively enhancing the control ability and improving the ecological structure of the soil to a greater extent. Studies have shown that both Trichoderma and Mortierella can be used as biocontrol strains to reduce the occurrence of Panax notoginseng root rot and alleviate the obstacles to continuous cropping of Panax notoginseng, but it is still unclear whether the two can be used in combination to synergistically improve the stress resistance of Panax notoginseng plants and alleviate the obstacles to continuous cropping of Panax notoginseng. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a composition comprising Trichoderma and Mortierella and application thereof in biological control of Panax notoginseng.

[0008] In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] Specifically, the first aspect of the present invention provides a composition for biological control of Panax notoginseng, wherein the composition comprises strains, conidia and / or volatiles of bacterial cultures of Trichoderma afroharzianum 3NG-1 and Mortierella alpina B28-1, wherein the Trichoderma afroharzianum 3NG-1 has been disclosed in Lifen Luo, et al, "Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng", Frontiers in Microbiology, Volume 10, Article 1597, July 2019, fig 7, and the Mortierella alpina B28-1 has been disclosed in the applicant's patent (CN116218683B), and the deposit number of Mortierella alpina B28-1 is CGMCC No.40006; classification name: Mortierella alpina; the preservation unit is the General Microbiology Center of China Culture Collection Administration; the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101; the preservation date is December 10, 2021.

[0010] As an optional manner, in the above composition, the volume ratio of the Trichoderma harzianum 3NG-1 to the Mortierella alpina B28-1 is 20:1 to 1:20.

[0011] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 10:1 to 1:10.

[0012] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 5:1 to 1:5.

[0013] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 4:1 to 1:1.

[0014] The second aspect of the present invention provides a preparation comprising the composition described in the first aspect.

[0015] As an optional mode, in the above preparation, the preparation is a mixed bacterial suspension.

[0016] As an optional method, in the above preparation, the concentration of the spore suspension of Trichoderma harzianum 3NG-1 and Mortierella alpina B28-1 is 1.0×10 6 ~1.0×10 8 CFU / mL.

[0017] As an optional method, in the above preparation, the concentration of the spore suspension of the Trichoderma harzianum 3NG-1 and the Mortierella alpina B28-1 is 1.0×10 7 CFU / mL.

[0018] The third aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for biological control of Panax notoginseng diseases.

[0019] As an optional mode, in the above application, the Panax notoginseng disease is root rot.

[0020] As an optional mode, in the above application, the Panax notoginseng disease is black spot disease.

[0021] The fourth aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for alleviating the soil continuous cropping effect during the growth of Panax notoginseng.

[0022] The fifth aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for promoting the growth of Panax notoginseng.

[0023] The sixth aspect of the present invention provides use of the composition described in the first aspect or the preparation described in the second aspect for preparing a biological control preparation.

[0024] The seventh aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for preparing a plant growth-promoting preparation.

[0025] As an optional manner, in the application described in the sixth aspect or the seventh aspect above, the preparation is used for Panax notoginseng.

[0026] The beneficial effects of the present invention are as follows:

[0027] There is no mutual antagonism between the alpine mortierella B28-1 and the African harzianum 3NG-1 provided by the present invention, and both strains can significantly inhibit the growth of four main root rot pathogens of Panax notoginseng, wherein the inhibition rate of African harzianum 3NG-1 on the four pathogens is more than 70%, and the alpine mortierella B28-1 has a good inhibitory effect on Fusarium solani F3, with an inhibition rate of 29.9%. After using 50 mL of a mixed spore suspension of alpine mortierella B28-1 and African harzianum 3NG-1 in a volume ratio of v (alpine mortierella B28-1): v (African harzianum 3NG-1) = 1:4 to treat the roots of Panax notoginseng for 48 hours, the leaves are inoculated with Panax notoginseng black spot pathogen Alternaria panax, and the result shows that the lesion area of ​​the Panax notoginseng leaves is significantly reduced. The spore suspension concentration was 1.0 × 10 6 and 1.0×10 7 CFU / mL, and the mixed spore suspensions in different volume ratios were used to irrigate the roots of Panax notoginseng. Compared with the sterilized water control, the mixed spore suspensions can increase the survival rate of Panax notoginseng and reduce the incidence of root rot. The volume ratio of v (Mortellini alpina B28-1): v (Trichoderma harzianum 3NG-1) = 4:1, and the concentrations of both bacteria were 1.0×10 7 CFU / mL, and mixed at v(Mortellini alpina B28-1):v(Trichoderma harzianum 3NG-1)=1:4, both of which could significantly promote the survival rate of Panax notoginseng and reduce the incidence of Panax notoginseng root rot. The other two bacteria concentrations were 1.0×10 7 CFU / mL, and when mixed at a ratio of v(alpine Mortierella B28-1):v(African Trichoderma harzianum 3NG-1)=1:1, the incidence of root rot of Panax notoginseng was significantly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The confrontation between Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on PDA medium. Among them, a is the control of Mortierella alpina B28-1, b is the control of Trichoderma harzianum 3NG-1, and c is the confrontation picture of the two strains.

[0029] Figure 2 The fresh weight growth of mycelium of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 after shaking culture on different concentrations of crude saponins of Panax notoginseng as the sole carbon source. Different lowercase letters in the figure indicate significant differences at the P<0.05 level tested by Duncan's new multiple range method.

[0030] Figure 3 The plate is a plate after 4-6 days of confrontation between Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 and the main root rot pathogens of Panax notoginseng Z5, F3, M, and RS6. Among them, a is a control of the confrontation between 4 pathogens and Mortierella alpina B28-1, b is a plate image of the confrontation between 4 pathogens and Mortierella alpina B28-1, c is a control of the confrontation between 4 pathogens and Trichoderma harzianum 3NG-1, and d is a plate image of the confrontation between 4 pathogens and Trichoderma harzianum 3NG-1.

[0031] Figure 4 The combination of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 enhances the resistance of Panax notoginseng plants to black spot disease. Different lowercase letters in the figure indicate significant differences at the P<0.05 level tested by Duncan's new multiple range method.

[0032] Figure 5 The effect of the combined use of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on the survival rate and incidence of Panax notoginseng. Different lowercase letters in the figure indicate significant differences at the P<0.05 level tested by Duncan's new multiple range method. DETAILED DESCRIPTION

[0033] The present invention will be further described with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0034] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0035] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are commercially available products unless otherwise specified.

[0036] Example 1 Evaluation of antagonism between Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1

[0037] The bacterial cakes were prepared from the strains of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 using a sterile cork puncher, and Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 were inoculated on PDA plates using the plate confrontation method, with a distance of about 5.5 cm between them. Each treatment was repeated 4 times, and the control was inoculated with Mortierella alpina B28-1 or Trichoderma harzianum 3NG-1 on one side and blank PDA agar blocks on the other side, and cultured in a constant temperature incubator at 28°C. The colony radius of the two strains was measured when the colonies of the two strains grew to be close to each other.

[0038] The results are shown in Table 1 and Figure 1 As shown, compared with the control, Mortierella alpina B28-1 significantly promoted the growth of Trichoderma africanum 3NG-1; there was no significant difference in the colony radius of Mortierella alpina B28-1, indicating that there was no mutual antagonism between the two strains.

[0039] Table 1 Antagonistic effects of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on the main pathogens of Panax notoginseng root rot

[0040]

[0041] Note: * indicates significant difference compared with the control at P<0.05 level after t-test.

[0042] Example 2 Utilization ability of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on crude saponins of Panax notoginseng

[0043] The strains of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 were shaken in different concentrations (0.01%, 0.1% and 1%) of crude saponin liquid culture medium, the composition of which was: 3g NaNO3, 1g KH2PO4, 0.5g KCl, 0.5g MgSO4·7H2O, 0.01g FeSO4· 7H2O , 0.1g (or 1g, 10g) crude saponin, 15g agar, 1000mL distilled water, dissolve, mix, and adjust pH to 7.0 with 1mol / L NaOH solution. The culture medium without crude saponin was used as the control. Alpine Mortierella B28-1 and African Trichoderma harzianum 3NG-1 were shaken at room temperature and 120rpm for 7d and 5d respectively, then the water was drained and the agar block was removed to weigh the fresh weight of mycelium. The results are as follows Figure 2As shown, compared with the control, Mortierella alpina B28-1 was able to grow using low concentrations of crude saponins, while high concentrations of crude saponins inhibited its growth; Trichoderma harzianum 3NG-1 was able to grow using notoginseng crude saponins at all three tested concentrations, especially at high concentrations.

[0044] Example 3 Inhibitory effects of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on the main pathogens of Panax notoginseng root rot

[0045] The alpine Mortierella B28-1 and African Trichoderma harzianum 3NG-1 were co-cultured with the main pathogens of Panax notoginseng root rot using plate confrontation. The pathogenic strains of Panax notoginseng were Cylindrocarpondestructans RS6, Fusarium solani F3, Fusarium oxysporum Z5, and Monographella cucumerina M. A sterile punch was used to prepare bacterial cakes from the alpine Mortierella B28-1 strain, African Trichoderma harzianum 3NG-1, and the tested Panax notoginseng pathogens, and the two strains were inoculated with the four pathogens on PDA culture medium, with a distance of about 5.5 cm between each other. Each treatment was repeated 5 times, and the control was inoculated with pathogens on one side and blank PDA agar blocks on the other side. The culture was carried out in a constant temperature incubator at 28°C. The colony radius of the pathogen was measured after 4-6 days, and the inhibition rate was calculated.

[0046] Inhibition rate (%) = (control colony radius - confrontation culture colony radius) / control colony radius × 100

[0047] The results are shown in Table 2 and Figure 3 As shown, the above-mentioned Mortierella alpina B28-1 and African Trichoderma harzianum 3NG-1 can significantly inhibit the growth of the main pathogens of Panax notoginseng root rot. Among them, Mortierella alpina B28-1 has a better inhibitory effect on Fusarium solani, with an inhibition rate of 29.9%, which is more than 3 times that of other pathogens. The inhibition rate of African Trichoderma harzianum 3NG-1 on the four pathogens is more than 70%.

[0048] Table 2 Antagonistic effects of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 on the main pathogens of Panax notoginseng root rot

[0049]

[0050] Example 4 Application of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 in combination to induce Panax notoginseng plants to enhance resistance to black spot disease

[0051] A mixed spore suspension of Mortierella alpina B28-1 strain and Trichoderma harzianum 3NG-1 is prepared, and the preparation method comprises the following steps:

[0052] 1) inoculating the Mortierella alpina B28-1 strain and the African Trichoderma harzianum 3NG-1 strain on PDA medium for culture, respectively, to obtain the Mortierella alpina B28-1 strain colonies and the African Trichoderma harzianum 3NG-1 strain colonies;

[0053] 2) Add 20 mL of sterile water to the culture dishes of the two strains in step 1), scrape the conidia on the surface, and filter with 4 layers of sterilized gauze to obtain conidia suspensions of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1, respectively. The concentrations of the two suspensions were adjusted to 1×10 7 CFU / mL;

[0054] 3) The two conidia suspensions in step 2) were fully mixed at a volume ratio of v (Mortierella alpina B28-1):v (Trichoderma harzianum 3NG-1) = 1:4.

[0055] The roots of Panax notoginseng were irrigated with 50 mL of a mixed conidia suspension of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 (7MT14), and treated with sterile water (CK) and single strain treatment (7M10: 10 mL of conidia suspension of Mortierella alpina B28-1 mixed with 40 mL of sterile water, 7T40: 40 mL of conidia suspension of Trichoderma harzianum 3NG-1 mixed with 10 mL of sterile water) for 48 hours. Then, the leaves were inoculated with Alternaria panax, the black spot pathogen of Panax notoginseng, and the lesion area was measured after the onset of the disease.

[0056] The control effect on the Panax notoginseng black spot pathogen Alternaria panax was calculated according to the formula: control effect (%) = (relative lesion area of ​​control - relative lesion area of ​​treatment) / relative lesion area of ​​control × 100%.

[0057] The synergistic ratio was calculated according to the Abbott method.

[0058] Efficiency ratio = Cobs / %Cexp = Cobs / [A+B-(AB / 100)]

[0059] Where: Cobs represents the actual control effect of the mixed bacterial suspension; %Cexp represents the expected control effect of the mixed bacterial suspension; A and B represent the actual control effects of two single bacterial agents on pathogens. If the synergistic ratio is >1, the mixed bacterial suspension exhibits a synergistic effect.

[0060] The results are as follows Figure 3As shown in Table 3, compared with the sterilized water control and the single strain conidia suspension, the lesion area treated with the mixed conidia suspension was significantly reduced, and the synergistic ratio of the mixed conidia suspension treatment was 8.64, indicating that the combination of the alpine Mortierella B28-1 strain and the African Trichoderma harzianum 3NG-1 synergistically enhanced the resistance of Panax notoginseng leaves to black spot disease.

[0061] Table 3 The synergistic ratio of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 in the control of Panax notoginseng leaf black spot

[0062]

[0063] Note: * indicates synergistic effect

[0064] Example 5 Application of Mortierella alpina B28-1 strain and Trichoderma harzianum strain 3NG-1 in alleviating the obstacle of continuous cropping of Panax notoginseng

[0065] A mixed spore suspension of Mortierella alpina B28-1 strain and Trichoderma harzianum 3NG-1 is prepared, and the preparation method comprises the following steps:

[0066] 1) inoculating the Mortierella alpina B28-1 strain and the African Trichoderma harzianum 3NG-1 strain on PDA medium for culture, respectively, to obtain the Mortierella alpina B28-1 strain colonies and the African Trichoderma harzianum 3NG-1 strain colonies;

[0067] 2) Add 20 mL of sterile water to the culture dishes of the two strains in step 1), scrape the conidia on the surface, and filter with 4 layers of sterilized gauze to obtain conidia suspensions of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1, respectively. The concentrations of the two suspensions were adjusted to 1×10 6 and 1×10 7 CFU / mL;

[0068] 3) The two conidia suspensions of the same concentration in step 2) are fully mixed at different volume ratios, as follows:

[0069] 6MT41: 1.0×10 6 The concentration of the spore suspension of CFU / mL was mixed at a ratio of v(alpine Mortierella B28-1):v(African Trichoderma harzianum 3NG-1)=4:1, and the single strain treatment was used as the control (6M40: the conidia suspension of the same concentration of the alpine Mortierella B28-1 strain was mixed at a ratio of v(alpine Mortierella B28-1):v(sterile water)=4:1; 6T10: the conidia suspension of the same concentration of the African Trichoderma harzianum 3NG-1 strain was mixed at a ratio of v(African Trichoderma harzianum 3NG-1):v(sterile water)=1:4);

[0070] 7MT41: 1.0×10 7 The concentration of the spore suspension of CFU / mL was mixed at a ratio of v(alpine Mortierella B28-1):v(African Trichoderma harzianum 3NG-1)=4:1, and the single strain treatment was used as the control (7M40: the same concentration of the conidia suspension of the alpine Mortierella B28-1 strain was mixed at a ratio of v(alpine Mortierella B28-1):v(sterile water)=4:1; 7T10: the same concentration of the conidia suspension of the African Trichoderma harzianum 3NG-1 strain was mixed at a ratio of v(African Trichoderma harzianum 3NG-1):v(sterile water)=1:4);

[0071] 7MT11: 1.0×10 7 The concentration of the spore suspension of CFU / mL was mixed at a ratio of v(alpine Mortierella B28-1):v(African Trichoderma harzianum 3NG-1)=1:1, and the single strain treatment was used as the control (7M25: the conidia suspension of the same concentration of the alpine Mortierella B28-1 strain was mixed at a ratio of v(alpine Mortierella B28-1):v(sterile water)=1:1; 7T25: the conidia suspension of the same concentration of the African Trichoderma harzianum 3NG-1 strain was mixed at a ratio of v(African Trichoderma harzianum 3NG-1):v(sterile water)=1:1);

[0072] 7MT14: 1.0×10 7 The concentration of the spore suspension of CFU / mL was mixed at a ratio of v(alpine Mortierella B28-1):v(African Trichoderma harzianum 3NG-1)=1:4, and the single strain treatment was used as the control (7M10: the same concentration of the conidia suspension of the alpine Mortierella B28-1 strain was mixed at a ratio of v(alpine Mortierella B28-1):v(sterile water)=1:4; 7T40: the same concentration of the conidia suspension of the African Trichoderma harzianum 3NG-1 strain was mixed at a ratio of v(African Trichoderma harzianum 3NG-1):v(sterile water)=4:1);

[0073] The continuously cropped Panax notoginseng was irrigated with 50 mL of the mixed conidia suspension of the above-mentioned Mortierella alpinia B28-1 and Trichoderma harzianum 3NG-1, and an equal volume of sterile water and a single strain conidia suspension were used as controls. Each treatment was replicated 5 times, once every 15 days, for a total of three root irrigations. The seedling survival rate and root rot incidence rate were counted 2 months after the first root irrigation treatment.

[0074] The combined synergistic ratio of the mixed conidia suspension of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 against Panax notoginseng root rot was calculated.

[0075] The control effect on Panax notoginseng root rot was calculated according to the formula: control effect (%) = (control incidence rate - treatment incidence rate) / control incidence rate × 100%.

[0076] The synergistic ratio was calculated according to the Abbott method.

[0077] Efficiency ratio = Cobs / %Cexp = Cobs / [A+B-(AB / 100)]

[0078] Where: Cobs represents the actual control effect of the mixed bacterial suspension; %Cexp represents the expected control effect of the mixed bacterial suspension; A and B represent the actual control effects of two single bacterial agents on pathogens. If the synergistic ratio is >1, the mixed bacterial suspension exhibits a synergistic effect.

[0079] The results are as follows Figure 4 As shown in Table 4, compared with the sterilized water control, the mixed conidia suspension of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 significantly reduced the incidence of root rot of Panax notoginseng and increased the survival rate of Panax notoginseng. After the spore suspension of the two strains mixed at a ratio of v (Mortierella alpina B28-1): v (sterilized water) = 4:1 was used to irrigate the roots of Panax notoginseng, the survival rate of Panax notoginseng was significantly higher than that of the single strain treatment. In terms of the incidence of root rot, the mixed spore suspension also showed a lower incidence. In addition, the synergistic ratios of the treatments of 6MT41, 7MT41 and 7MT14 were all greater than 1, indicating that the combined synergistic effect of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 reduced the incidence of root rot of Panax notoginseng. In short, the combined use of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 has a better synergistic effect than single strains, and further alleviates the obstacles to continuous cropping of Panax notoginseng.

[0080] Table 4 The synergistic ratio of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 in the control of Panax notoginseng root rot

[0081]

[0082] Note: * indicates synergistic effect

[0083] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for biological control of Panax notoginseng, characterized in that: The composition comprises strains, conidia and / or volatiles of bacterial cultures of Trichoderma afroharzianum 3NG-1 and Mortierella alpina B28-1.

2. The composition according to claim 1, characterized in that: The volume ratio of the African Trichoderma harzianum 3NG-1 to the Mortierella alpina B28-1 is 20:1 to 1:

20.

3. A preparation comprising the composition of claim 1 or claim 2.

4. The preparation according to claim 3, characterized in that: The preparation is a mixed bacterial suspension.

5. The preparation according to claim 4, characterized in that: The concentration of the spore suspension of Trichoderma harzianum 3NG-1 and Mortierella alpina B28-1 was 1.0×10 6 ~1.0×10 8 CFU / mL.

6. Use of the composition of claim 1 or claim 2 or the preparation of any one of claims 3 to 5 for biological control of Panax notoginseng diseases.

7. The use according to claim 6, characterized in that: The Panax notoginseng disease is root rot or black spot.

8. Use of the composition of claim 1 or claim 2 or the preparation of any one of claims 3 to 5 for alleviating the soil continuous cropping effect during the growth of Panax notoginseng.

9. Use of the composition of claim 1 or claim 2 or the preparation of any one of claims 3 to 5 for promoting the growth of Panax notoginseng.

10. Use of the composition according to claim 1 or claim 2 or the preparation according to any one of claims 3 to 5 for preparing a biological control preparation or a plant growth promotion preparation.

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