Hericium erinaceus

A technology of Hericium erinaceus and Hericium erinaceus is applied in the cultivation field of edible fungi, which can solve the problem of low biological yield of mycelium, and achieve the effects of high yield, fast growth rate and high biological yield.

Inactive Publication Date: 2012-04-04
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to solve the problem of low mycelium biological yield in the pro

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0027] Example 1 Screening of Hericium erinaceus 1 strains

[0028] Using 32 Hericium erinaceus strains (3 from the Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences; 4 from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; others cited from the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Fujian Sanming Institute of Fungi, Jilin Provinces, Jiangsu Province and other places (the specific location is not known), the 32 Hericium erinaceus strains are all wild species) as materials, in Difco PDA TM Culture medium (a standard medium, produced in Germany) at 25±0.1℃ for 7 days, measure the colony diameter, the growth rate of mycelium = colony diameter / 7d, the results (see Table 1) show that the Chinese Academy of Agricultural Sciences Agricultural Resources and Agriculture The No. 18 strain collected from Changbai Mountain in Northeast China by the Institute of Reg...

Example Embodiment

[0032] Example 2 Identification of Nong Hericium 1 in Hericium erinaceus strains

[0033] The No. 18 Hericium erinaceus strain selected in Example 1, namely "Zhongnong Hericium 1" was identified. Its morphological characteristics were: the hyphae were white and dense, the hyphae were suitable at pH 4 to 5, and the temperature was 24 to 26°C. Grow under the conditions, the growth rate of mycelium is 5.8mm / d on Difco PDA medium (a standard medium, produced in Germany) at 25°C. "Zhongnong Hericium 1" is a low-medium temperature fruiting variety, with high quality, high yield and strong stress resistance. The optimum temperature during the fruiting period is 10-22℃, and the optimum temperature is 17±1℃. The fruit body is single, pure white; the diameter of the fruit body is 10cm~30cm, medium solid; the stipe length is 2~4cm, and the thorn length is 2~5cm.

[0034] ITS sequence sequencing

[0035] The total DNA of Zhongnong Hericium 1 was extracted by the CTAB method, the total DNA was...

Example Embodiment

[0044] Example 3 Submerged fermentation culture of Hericium erinaceus 1 mycelium

[0045] Follow the steps below:

[0046] (1). The strain is activated under aseptic conditions from Zhongnong Hericium 1 (preserved in the General Microbiology Center of the China Microbial Culture Collection and Management Committee on December 23, 2009, the deposit number is: CGMCC3536) mother seed test tube Take out the mycelium block of size 2-4mm×2-4mm, quickly inoculate it into the slant medium, plug it with silica gel plug, and incubate it in an incubator at 25°C for 15 days to obtain the activated Zhongnong Hericium 1 strain. The slant medium is prepared according to the following method: peel the potatoes, weigh 200 g, cut into small pieces of about 1 cm3, add water and boil until the potatoes are soft and not rotten, and filter with 4 layers of gauze. Take the filtrate, add 20g glucose and 20g agar, heat it until the agar is completely melted, keep stirring during this time to prevent the p...

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Abstract

The invention discloses a novel Hericium erinaceus bacterial strain 1 of Hericium erinaceus, which has the advantages that: the biological yield of the novel Hericium erinaceus mycelium in the Hericium erinaceus bacterial strain 1 which is obtained in a culture method disclosed by the invention is high and can reach 12g/L which is twice of the fermentation production capacity in present factories; and the growth speed of the novel Hericium erinaceus bacterial strain 1 is fast and can reach 5.8mm/d, and the yield is high.

Description

[0001] The present invention is a divisional application of the patent application with the application number: 201010109627.2, the application date: 2010.2.9, and the invention title "The liquid submerged fermentation method of Hericium erinaceus". Technical field: [0002] The invention belongs to a cultivation method of edible fungi, in particular to a liquid submerged fermentation cultivation method of Hericium erinaceus, and also relates to a medium for fermentation cultivation. A Hericium erinaceus is also involved. Background technique: [0003] Hericium erinaceus is named for its physical shape resembling the head of a small monkey. Also known as Hericium erinaceus, Hericium erinaceus, Lemon bacteria, Tricholoma erinaceus, Hebala (Tibetan name), Shanfu mushroom (Japan) and Bear head seedling (Europe). (Lv Zuozhou, Edible Fungi Cultivation, Higher Education Press, 2006, P262). Hericium erinaceus is a rare edible fungus with edible and medicinal value. Hericium erina...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 张金霞黄晨阳陈强高巍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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