Heterotrophic culture medium used for large-scale cultivation of marine microalgae
A technology for large-scale cultivation and marine microalgae, applied in the field of marine microalgae, can solve the problems of long doubling time, low algae cell density, and high cost
Inactive Publication Date: 2017-04-26
青岛力天宏泰新能源科技有限公司
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Problems solved by technology
[0004] At present, the large-scale cultivation of marine microalgae is mainly carried out through photoautotrophy. The main problems are that the growth cycle of algae cells is slow, the doubling time is long, the density of algae cells in the plateau stage is low, and the culture medium has many components and high cost.
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[0015] Now in conjunction with specific embodiment and cultivation effect experiment, the present invention is elaborated:
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Abstract
The invention relates to the field of marine microalgae, in particular to a heterotrophic culture medium used for large-scale cultivation of marine microalgae. The culture medium is prepared from 0.5-1.5 g of nitrate, 2-10 mg of monopotassium phosphate, 2-10 mg of dipotassium phosphate, 0.1-1 mg of ferric citrate, 0.5-2 mg of magnesium sulfate heptahydrate, 0.5-2 mg of calcium chloride dehydrate, 5-20 g of an organic carbon source and 1000 mL of filtered seawater. The nitrate is potassium nitrate or sodium nitrate, and the organic carbon source is glucose or sucrose. The heterotrophic culture medium is widely used for the large-scale cultivation of the marine microalgae, can increase growth velocity of the marine microalgae, shorten the growth cycle of the marine microalgae, increase the biological yield of the marine microalgae, meanwhile sharply reduce the cost of the culture medium, and is an ideal culture medium used for the large-scale cultivation.
Description
technical field [0001] The invention relates to the field of marine microalgae, in particular to a heterotrophic culture medium for large-scale cultivation of marine microalgae. Background technique [0002] With the progress of society and the development of science and technology, people's exploitation and utilization of land resources has gradually reached the limit, and more and more countries have begun to focus on the ocean, which accounts for 71% of the earth's surface. Because there are abundant algae resources in the ocean, and they are widely sourced, easy to cultivate, reproduce rapidly, and produce rich products, they are widely used in aquaculture, food processing, medical beauty, and bioenergy. Microalgae (Microalgae) is the opposite of macroalgae, the tiny algae whose shape is invisible to the naked eye, including eukaryotes and prokaryotes, accounting for about 70% of the total algae, is an important economic and ecological algae . [0003] Marine microalga...
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IPC IPC(8): C12N1/12
Inventor 王颖
Owner 青岛力天宏泰新能源科技有限公司
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