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Liquid submerged fermentation culture method for hericium erinaceus

A technology of liquid submerged fermentation and culture method, which is applied in the field of cultivation of edible fungi, can solve the problems of low biological yield of mycelia, and achieve the effects of high yield, good effect and fast growth speed

Inactive Publication Date: 2012-01-11
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to solve the problem of low mycelium biological yield in the production method of Hericium erinaceus, and provide a liquid submerged fermentation method for Hericium erinaceus

Method used

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  • Liquid submerged fermentation culture method for hericium erinaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of Hericium erinaceus 1 strains

[0027] Using 32 Hericium erinaceus strains (3 from the Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences; 4 from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; others cited from the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Fujian Sanming Institute of Fungi, Jilin Provinces, Jiangsu Province and other places (the specific location is not known), the 32 Hericium erinaceus strains are all wild species) as materials, in Difco PDA TM Culture medium (a standard medium, produced in Germany) at 25±0.1℃ for 7 days, measure the colony diameter, the growth rate of mycelium = colony diameter / 7d, the results (see Table 1) show that the Chinese Academy of Agricultural Sciences Agricultural Resources and Agriculture The No. 18 strain collected from Changbai Mountain in Northeast China by the Institute of Regi...

Embodiment 2

[0030] Example 2 Identification of Nong Hericium 1 in Hericium erinaceus strains

[0031] The No. 18 Hericium erinaceus strain selected in Example 1, namely "Zhongnong Hericium 1" was identified. Its morphological characteristics were: the hyphae were white and dense, the hyphae were suitable at pH 4 to 5, and the temperature was 24 to 26°C. Grow under the conditions, the growth rate of mycelium is 5.8mm / d on Difco PDA medium (a standard medium, produced in Germany) at 25°C. "Zhongnong Hericium 1" is a low-medium temperature fruiting variety, with high quality, high yield and strong stress resistance. The optimum temperature during the fruiting period is 10-22℃, and the optimum temperature is 17±1℃. The fruit body is single, pure white; the diameter of the fruit body is 10cm~30cm, medium solid; the stipe length is 2~4cm, and the thorn length is 2~5cm.

[0032] ITS sequence sequencing

[0033] The total DNA of Zhongnong Hericium 1 was extracted by the CTAB method, the total DNA was...

Embodiment 3

[0042] Example 3 Submerged fermentation culture of Hericium erinaceus 1 mycelium

[0043] Follow the steps below:

[0044] (1). The strain is activated under aseptic conditions from Zhongnong Hericium 1 (preserved in the General Microbiology Center of the China Microbial Culture Collection and Management Committee on December 23, 2009, the deposit number is: CGMCC3536) mother seed test tube Take out the mycelium block of size 2-4mm×2-4mm, quickly inoculate it into the slant medium, plug it with silica gel plug, and incubate it in an incubator at 25°C for 15 days to obtain the activated Zhongnong Hericium 1 strain. The slant medium is prepared according to the following method: peel the potatoes, weigh 200 g, cut into small pieces of about 1 cm3, add water and boil until the potatoes are soft and not rotten, and filter with 4 layers of gauze. Take the filtrate, add 20g glucose and 20g agar, heat it until the agar is completely melted, keep stirring during this time to prevent the p...

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Abstract

The invention discloses a liquid submerged fermentation culture method for hericium erinaceus, and belongs to the field of culture methods for edible fungi. The culture method comprises the following steps of: activating hericium erinaceus strains on a slant culture medium, then performing primary strain culture on a liquid seed culture medium, performing secondary strain culture on a liquid fermentation culture medium, and finally performing amplified culture on the liquid fermentation culture medium to obtain hericium erinaceus mycelium. The invention also discloses hedgehog hydnum 1 in thehericium erinaceus strains, a seed culture medium and a fermentation culture medium for hericium erinaceus culture. The biological yield of the hericium erinaceus mycelium obtained by the culture method is high and can reach 12g / L which is over two times as much as the fermentation production level of the current factory; the hedgehog hydnum 1 in the hericium erinaceus strains has high growth speed which can reach 5.8 millimeters every day; and moreover, the liquid seed culture medium and the fermentation culture medium have good culture effect so that the hericium erinaceus mycelium has quick growth and high yield.

Description

Technical field [0001] The invention belongs to a culture method of edible fungi, in particular to a liquid submerged fermentation culture method of Hericium erinaceus, and also relates to a culture medium for fermentation culture. Background technique [0002] Hericium erinaceus, named after its entity shape resembling the head of a little monkey. Also known as Hericium erinaceus, Hericium erinaceus, Pseudomonas, Tricholoma edulis, Hebala (Tibetan name), Mountain Fusarium (Japan) and Bear Head Miao (Europe). (Lu Zuozhou, Edible Fungus Cultivation, Higher Education Press, 2006, P262). Hericium erinaceus is a valuable edible fungus with both edible and medicinal value. Hericium erinaceus polysaccharide can obviously inhibit the growth of stomach, esophagus, liver and skin cancers (Mizuno, T. International Journal of Medicinal Mushrooms 1, 1999. p105-119.) It has three main active ingredients: β-D-glucan, which has anti-tumor effect; ergosterol, which is a precursor of vitamin D;...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 张金霞黄晨阳陈强高巍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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