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Sipunculus nudus ACE inhibitory peptide as well as preparation method and application thereof
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The technology of the worm and the inhibitory peptide is applied in the field of ACE inhibitory peptide and its preparation, which can solve the problem of high production cost, and achieve the effects of small processing volume, suitable for human body absorption, and good antihypertensive effect.
Active Publication Date: 2021-04-13
MARINE MEDICAL RES INST OF GUANGDONG ZHANJIANG
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Problems solved by technology
Most of the existing antihypertensive drugs are artificially synthesized chemical drugs, which are prone to produce various side effects during clinical application, and the production cost is relatively high
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Embodiment 1
[0039]1. Preparation and purification of the inhibitory peptide in the square starworm ACE
[0040](1) Fresh square star flip to remove the visceral, washed, weigh 500g, chopping; add pure water 5L, mix, adjust the pH 7.5-9.5 with 1% sodiumhydroxide solution; Enzymepowder (trypsin-neutral protease = 3: 1, w / w) plus pure water is pulled into milk, produced into enzymepowder; the enzyme is slowly added to Fangxing according to 0.5% -1% of the square of the square. In the insect liquid, the addition is mixed and mixed, and the temperature is 45 ° C, the enzymatic solution is 4-6h;
[0041]With a pH of 7.5, an enzyme is 1%, an enzymatic solution is 4h, and the ACE inhibition rate of the enzymatic hydrolysis is a single factor experiment. Each time a parameter is changed, the pH is 7.5, 8.5, 9.5, and the amount of enzyme is 0.5%, 0.8%, 1%, and the enzymatic solution of 4 h, 5h, 6h on the enzymatic solution is 4 h, 5 h, and 6h, and the optimal enzyme is determined by orthogonal experiment....
Embodiment 2
[0056]Preparation of Fangx Star Phythectic Petropeptide
[0057]1 formula
[0058]Ingredients: 10-30% of the square starpococcal antihypertensope peptide
[0063]Mix the main material, excipient, sweetener, and sourness, slowly spray 50% -70% ethanol as a wetting agent to the mixture, while continuously stirring evenly, making a soft and hard soft material. Tools with 18 mesh sieves, pressed 18 mesh sieves, so that the soft material became a granular preparation soft material. Software requires a group, and it is touched. After the soft film is dried, the species of the Star Star Pattermide is chewable.
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Abstract
The invention discloses sipunculus nudusACE inhibitorypeptide as well as a preparation method and application thereof, and belongs to the technical field of biology. Through a single factor experiment and an orthogonal experiment, the optimal process parameters of enzymolysis of sipunculus nudus are determined as compound enzymepowder (the ratio of pancreatin to neutral protease is 3: 1), the material-liquid ratio is 1: 10, the mass ratio of enzyme to a substrate is 1%, the enzymolysis pH is 9.5, the enzymolysis temperature is 45 DEG C, the enzymolysis time is 4h, and an enzymolysis product is separated and purified through metal chelating chromatographic columnchromatography. and three polypeptide amino acid sequences are obtained through high performance liquid chromatography-tandem mass spectrometry identification, and a sipunculus nudus antihypertensive peptide buccal tablet is prepared from the obtained ACE inhibitorypeptide. The invention provides the new sipunculus nudus antihypertensive peptide, determines an optimal preparation process, and lays a foundation for further preparation of drugs with an antihypertensive effect.
Description
Technical field[0001]The present invention relates to the field of biotechnology, in particular to a square star inducera ACE inhibitory peptide and a preparation method thereof.Background technique[0002]With the development of my country's economy, the people's living standards continue to increase, and the number of patients with hypertension in my country is increasing. According to estimation, the number of patients with hypertension in my country in 2020 may exceed 300 million. There are many chemical drugs that have been synthesized in conges and composite drugs, which are prone to multiple side effects in the clinical application, and the production cost is higher. Therefore, biohylactic peptides with low cost, high safety, and good antihypertensive effect have become the focus of recent years.[0003]Due to the particularity of the marine ecological environment, such as high pressure, low temperatures, etc.), marine biomass proteins have a variety of specific amino acids and t...
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