Sipunculus nudus somatic protein peptide extract and preparation method thereof

The technology of the worm and protein peptide is applied in the field of extraction of active biological components, which can solve the problem of not being able to give full play to the medicinal value of worm, and achieve the effects of enriching product types, being easy to control, and being easy to absorb.

Inactive Publication Date: 2019-08-16
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventor describes an extraction technique called Sonoda pachystrophy (SP) from Chinese medicine plant Pygostigma cathayana Linn., where SP can be used alone or together with other materials like proteins such as bovine serum albumen. By crushing this tissue into small pieces, we get tiny amounts of these molecules made up mostly of amino acids without compromising their biological activity. These extracted fragments are useful because they contain various substances important for human health but difficult to break down easily due to strong bond between them. They provide potential benefits over existing methods including safer processing techniques, easier handling during manufacturing processes, less expensive products, and more efficient commercial production at reduced costs.

Problems solved by technology

The technical problem addressed by this patented product relates to improving its effectiveness on promoting healthy growth or prevention against diseases caused by lack of certain substances found naturally within stars called spinosynnema (a type of mushroom).

Method used

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  • Sipunculus nudus somatic protein peptide extract and preparation method thereof
  • Sipunculus nudus somatic protein peptide extract and preparation method thereof
  • Sipunculus nudus somatic protein peptide extract and preparation method thereof

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preparation example Construction

[0034] The preparation method of the extract of the body wall protein peptide of Spina gridia provided by the embodiment of the present invention comprises the following steps:

[0035] Prepare the body wall of the live star worm;

[0036] Pulverizing and homogenizing the body wall of the phalaenopsis to obtain a homogenate liquid of the body wall of the phalaenopsis;

[0037] Perform ultrasonic treatment on the homogenate of the body wall of the phalaenopsis to obtain an ultrasonic treatment solution;

[0038] Using protease to enzymatically hydrolyze the ultrasonic treatment solution to obtain an enzymolyzed solution;

[0039] The enzymolysis solution is sequentially inactivated and centrifuged to obtain a supernatant enzymolysis solution;

[0040] The supernatant enzymolysis solution is freeze-dried to obtain the extract of the body wall protein peptide of P. grisa.

[0041] In the preparation method of the extract of the body wall protein peptides of the scatterworm pro...

Embodiment 1

[0065] Select fresh and vigorous star worms, wash them and feed them in light salt water with a salt content of 30‰, and feed them with light salt water every 1 hour until they spit out all the sand (you can observe the amount of water in the feeding process). Change whether there is sediment in the light salt water to judge whether the sediment is spit out).

[0066] Take out the checkered starworm that has spit out all the sand, rinse it with sterile water, absorb the water on the surface of the checkered starworm with filter paper, cut the checkered starworm, and use a blunt bamboo stick or steel needle to remove the checkered starworm. The worms are turned over from the tail to the snout, and the viscera of the phalaenopsis is removed until there is no residue, and washed to obtain the body wall of the phalaenopsis.

[0067] Putting the cleaned body wall of the phalaenopsis into a meat grinder to grind, and then putting it into a homogenizer to homogenize it into a homogen...

Embodiment 2

[0073] Select fresh and vigorous gridworms, wash them and feed them in light salt water with a salt content of 30‰, and feed them with light salt water every 1 hour until they spit out all the sand (you can observe the feeding of gridworms Whether there is sediment in the light salt water of the worm).

[0074] Take out the checkered starworm that has spit out all the sand, rinse it with sterile water, absorb the water on the surface of the checkered starworm with filter paper, cut the checkered starworm, and use a blunt bamboo stick or steel needle to remove the checkered starworm. The worms are turned over from the tail to the snout, and the viscera of the phalaenopsis is removed until there is no residue, and washed to obtain the body wall of the phalaenopsis.

[0075] Putting the cleaned body wall of the phalaenopsis into a meat grinder to grind, and then putting it into a homogenizer to homogenize it into a homogenate to obtain a homogenate liquid of the body wall of the ...

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Abstract

The invention applies to the technical field of extraction of effective ingredients from organisms and discloses Sipunculus nudus somatic protein peptide extract and a preparation method thereof, wherein the preparation method comprises the steps of preparing Sipunculus nudus body wall from live Sipunculus nudus; crushing and homogenizing the Sipunculus nudus body wall to obtain Sipunculus nudus body wall homogenate; subjecting the Sipunculus nudus body wall homogenate to ultrasonic cell breaking to obtain an ultrasonically treated liquid; enzymatically hydrolyzing the ultrasonically treated liquid with proteinase to obtain enzymatic hydrolysate; subjecting the enzymatic hydrolysate to enzyme deactivation, and centrifuging to obtain enzymatic hydrolyzed supernate; lyophilizing the enzymatic hydrolyzed supernate to obtain Sipunculus nudus somatic protein peptide extract. The preparation method of the invention has the advantages of high safety, mild production conditions, good convenience of control, good process simplicity, good convenience of industrial production, low cost and the like; the prepared extract has high hydrolytic degree and high protein peptide content.

Description

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Claims

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Application Information

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Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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