Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus

Abstract

The invention discloses a colony-family-molecule comprehensive genetic breeding method for takifugu obscures. The colony-family-molecule comprehensive genetic breeding method for takifugu obscures comprises the following steps: firstly, continuously selecting and breeding for four generations, after selecting and breeding the colony, taking two colonies with obvious segregation of character as the basis selecting and breeding colonies for the next generation for family selecting and breeding, adopting a molecular marker technology for establishing the assistant family, breeding solely after the generation of each family parent, establishing the basis colony taking the family as the unit and forming a larger production unit, selecting and breeding for each independent production unit, selecting for six time during three breeding stages, in the marketable fish breeding stage and before the maturing stage, and repeatedly performing the steps for selecting and breeding the nest generation. The colony-family-molecule comprehensive genetic breeding method for takifugu obscures has the advantages that the advantage parent can be effectively selected, the molecular marker technology is adopted for executing the genetic analysis, the genetic conditions of the parent before the breeding stage can be understood for guiding the parent pairing and combining the scientific research with the production, the advantage individual in the family can be effectively selected, and the optimization breeding and the resource utilization can be achieved.

Description

technical field [0001] The invention relates to a method for selecting and breeding improved varieties of aquaculture species, in particular to a method for fugu obscurus group-family-molecular comprehensive genetic breeding. technical background [0002] Pufferfish obscurus is of the order Fuguformes ( Tetraodontiformes ), Pufferinae ( Tetraodontoidei ), Pufferidae ( Tetraodontidae ), Fugu ( Takifugu ). At present, Shanghai, Jiangsu, Zhejiang, Anhui, Guangdong, Fujian, Sichuan, Henan, Hubei, Hainan and other provinces and cities across the country have carried out the cultivation of freshwater puffer obscurus, and puffer obscurus has gradually become a famous and high-quality species for breeding. It is one of the aquatic products with relatively high economic and nutritional value. However, with the continuous cultivation of general cultured species, germplasm resource degradation and inbreeding depression will inevitably occur. Therefore, in order to fundamentally...

AI Technical Summary

Problems solved by technology

However, family selection is to select a pair of parents to self-cross to produce offspring, the gene pool will continue to decrease, and when the size of the family breeding population is not large enough, the genes for good traits cannot be fully expressed, which affects the selection effect

Molecular marker-assisted breeding has the following advantages: multiple genes can be targeted at the same time for material screening, and multiple genes can be aggregated into the same breeding material to improve its quality; target traits can be screened in advance; DNA can be reflected Therefore, it can be selected at various stages of growth without being affected by the environment, greatly improving the efficiency and accuracy of target gene selection, and broadening the methods and approaches of breeding. However, the development of molecular markers It is cumbersome, the workload is heavy, and the functions of many genes have not yet been clarified, which has certain obstacles to breeding