A method of population-family-molecular comprehensive genetic breeding of pufferfish

Abstract

A method for fugu obscura group-family-molecular comprehensive genetic breeding, comprising the steps of: first carrying out group breeding for four consecutive generations, after the group selection, two populations with relatively obvious separation of traits appear as the next step of family selection With the help of molecular marker technology to assist the construction of the family, the offspring produced by the parents of each family are raised separately, and the basic population with the family as the unit is established to form a large production unit, and each independent production unit is grouped For selection and breeding, six selections are carried out in the three breeding stages, the commercial fish cultivation stage and before maturity, and the above steps are repeated for the next generation of breeding. The advantages of the present invention are: the dominant parent can be efficiently selected, and at the same time, genetic analysis can be carried out by using molecular marker technology, the genetic status of the parents can be understood before breeding, and the pairing of the parents can be guided, so as to achieve the purpose of combining scientific research and production, and Effectively realize the selection of dominant individuals in the family, and combine optimal breeding and resource utilization.

Description

technical field [0001] The invention relates to a method for selecting and breeding improved varieties of aquaculture species, in particular to a method for fugu obscurus group-family-molecular comprehensive genetic breeding. technical background [0002] Pufferfish obscurus is of the order Fuguformes ( Tetraodontiformes ), Pufferinae ( Tetraodontoidei ), Pufferidae ( Tetraodontidae ), Fugu ( Takifugu ). At present, Shanghai, Jiangsu, Zhejiang, Anhui, Guangdong, Fujian, Sichuan, Henan, Hubei, Hainan and other provinces and cities across the country have carried out the cultivation of freshwater puffer obscurus, and puffer obscurus has gradually become a famous and high-quality species for breeding. It is one of the aquatic products with relatively high economic and nutritional value. However, with the continuous cultivation of general cultured species, germplasm resource degradation and inbreeding depression will inevitably occur. Therefore, in order to fundamentally...

AI Technical Summary

Problems solved by technology

However, family selection is to select a pair of parents to self-cross to produce offspring, the gene pool will continue to decrease, and when the size of the family breeding population is not large enough, the genes for good traits cannot be fully expressed, which affects the selection effect

Molecular marker-assisted breeding has the following advantages: multiple genes can be targeted at the same time for material screening, and multiple genes can be aggregated into the same breeding material to improve its quality; target traits can be screened in advance; DNA can be reflected Therefore, it can be selected at various stages of growth without being affected by the environment, greatly improving the efficiency and accuracy of target gene selection, and broadening the methods and approaches of breeding. However, the development of molecular markers It is cumbersome, the workload is heavy, and the functions of many genes have not yet been clarified, which has certain obstacles to breeding