Molecular marker for genetic sex identification of Chinese soft-shell turtles and method

An identification method and technology for Chinese soft-shelled turtles, which are applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. High success rate, increase breeding output, and the effect of early parthenogenesis
CN109680076AActive Publication Date: 2019-04-26YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2019-04-26

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Abstract

The invention belongs to the technical field of aquatic product biology and discloses a molecular marker for genetic sex identification of Chinese soft-shell turtles and a method. The method comprisesthe steps: sequencing male and female individuals of the Chinese soft-shell turtles by adopting a RAD technology, and screening male and female specific fragments; carrying out female genome Walkingaccording to the male and female specific fragments, so as to obtain a specific fragment SEQ ID NO: 1 of 6532bp; designing primers on the basis of the specific fragment, and carrying out primer screening and verification, so as to obtain specific primers; acquiring calipash tissue of the Chinese soft-shell turtles, extracting genomic DNA from the Chinese soft-shell turtles, and carrying out amplification on Chinese soft-shell turtle individuals by using primer pairs; subjecting an amplification result to cataphoresis detection; carrying out genetic sex identification. According to the molecular marker and the method, the problem that gender-specific markers of the Chinese soft-shell turtles are not applied to genetic sex identification yet at present is solved, and thus, the molecular marker and the method are original. The success rate of accurate identification reaches 100%.
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Description

technical field

[0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a molecular marker and a method for genetic sex identification of Chinese soft-shelled turtle. Background technique

[0002] At present, the existing technologies commonly used in the industry are as follows:

[0003] The Chinese soft-shelled turtle (Pelodiscus sinensis) belongs to the class Reptilia, the order Turtle, the family Turtle, and the genus, and is commonly known as soft-shelled turtle, water fish, and turtle. In the world, soft-shelled turtles are mainly distributed in Asia, Africa and North America. In Asia, they are mainly distributed in China, eastern Russia, Korea, Japan and Vietnam. It is widely distributed in my country, and it is distributed in all provinces, cities and autonomous regions except Tibet, Qinghai and Xinjiang. Because of its high nutritional value and medicinal value, it has always been loved by consumers. In recent years,...

Claims

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