Molecular marker for genetic sex identification of Chinese soft-shell turtles and method

Abstract

The invention belongs to the technical field of aquatic product biology and discloses a molecular marker for genetic sex identification of Chinese soft-shell turtles and a method. The method comprisesthe steps: sequencing male and female individuals of the Chinese soft-shell turtles by adopting a RAD technology, and screening male and female specific fragments; carrying out female genome Walkingaccording to the male and female specific fragments, so as to obtain a specific fragment SEQ ID NO: 1 of 6532bp; designing primers on the basis of the specific fragment, and carrying out primer screening and verification, so as to obtain specific primers; acquiring calipash tissue of the Chinese soft-shell turtles, extracting genomic DNA from the Chinese soft-shell turtles, and carrying out amplification on Chinese soft-shell turtle individuals by using primer pairs; subjecting an amplification result to cataphoresis detection; carrying out genetic sex identification. According to the molecular marker and the method, the problem that gender-specific markers of the Chinese soft-shell turtles are not applied to genetic sex identification yet at present is solved, and thus, the molecular marker and the method are original. The success rate of accurate identification reaches 100%.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a molecular marker and a method for genetic sex identification of Chinese soft-shelled turtle. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] The Chinese soft-shelled turtle (Pelodiscus sinensis) belongs to the class Reptilia, the order Turtle, the family Turtle, and the genus, and is commonly known as soft-shelled turtle, water fish, and turtle. In the world, soft-shelled turtles are mainly distributed in Asia, Africa and North America. In Asia, they are mainly distributed in China, eastern Russia, Korea, Japan and Vietnam. It is widely distributed in my country, and it is distributed in all provinces, cities and autonomous regions except Tibet, Qinghai and Xinjiang. Because of its high nutritional value and medicinal value, it has always been loved by consumers. In recent years,...

AI Technical Summary

Problems solved by technology

[0007] (1) Usually in production practice, the existing conventional technology is mainly to identify the physiological sex of the soft-shelled turtle through the appearance characteristics of the soft-shelled turtle. good

[0008] (2) In scientific research, the physiological sex of soft-shelled turtle can also be identified by means of tissue slices, etc., but it is necessary to dissect and collect gonad tissue, resulting in the death of soft-shelled turtle

[0009] (3) In the absence of sex reversal, the genetic sex is consistent with the biological sex, and the genetic sex can be indirectly identified through the biological sex; but if it is a sex-reversed individual, the genetic sex is inconsistent with the biological sex, and the genetic sex cannot be obtained through the identification of the biological sex

[0010] (3) In the study of sex control of soft-shelled turtles, there may be sex-reversed individuals and non-sex-reversed individuals in the Chinese soft-shelled turtle population, and the true genetic sex cannot be obtained by shape identification and tissue section identification

[0012] The genetic sex of soft-shelled turtle can be accurately determined by developing accurate and efficient sex-specific molecular markers. At present, although there are many technical methods for the development of sex-specific markers, some technical methods can also be realized, and there are no significant technical obstacles. However, in the development of molecular markers There is a lot of uncertainty in the process, which is closely related to the population of soft-shelled turtles used, the type of molecular markers to be screened, the analysis method used, the setting of parameter thresholds, etc.

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