Citrus double-factor anti-allergy plant extract as well as preparation method and application thereof

Abstract

The invention discloses a citrus two-factor anti-allergy plant extract and a preparation method and application thereof. The citrus two-factor anti-allergy plant extract comprises poncirin and naringin in a mass ratio of (20-25): (6-10). The preparation method of the citrus double-factor anti-allergy plant extract comprises the following steps: carrying out heating reflux on crushed citrus fruits in a 50-80% ethanol solution for 5-8 hours to obtain an extracted crude product; slowly adding an extracted crude product to the column, and controlling the flow rate to be 1-2BV / h; and then eluting with 3-5BV distilled water to remove water-soluble impurities. And eluting with a 30-40% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain the naringin. And eluting with a 50-70% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain poncirin. When the plant extract is used in cosmetics, the cosmetics can have good moisturizing, soothing and other effects.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cosmetics, and particularly relates to a citrus double-factor anti-allergic plant extract and a preparation method and application thereof. BACKGROUND

[0002] With the improvement of people's living standards and the upgrading of consumption concepts, consumers' demand for cosmetics has gradually shifted from basic cleaning and modification functions to functional products with multiple functions such as moisturizing, soothing, anti-allergic and antioxidant. Especially under the influence of factors such as environmental pollution, increasing life pressure and irregular work and rest, skin sensitivity problems are increasingly common, and the demand for cosmetics with soothing and anti-allergic effects is increasingly urgent for sensitive skin groups.

[0003] At present, the commonly used anti-allergic ingredients on the cosmetic market mainly include chemical synthetic anti-allergic agents (such as allantoin, panthenol, glycyrrhizic acid dipotassium, etc.) and plant-derived anti-allergic ingredients (such as chamomile extract, aloe extract, green tea extract, etc.). Although the chemical synthetic anti-allergic agents have a faster effect, some components may have certain irritation, and long-term use may cause potential burden to the skin; and the traditional plant-derived anti-allergic ingredients, although having the advantages of natural mildness, most of them have the problems of single efficacy and limited anti-allergic effect, which is difficult to meet the demand of consumers for high-efficiency anti-allergic cosmetics. SUMMARY

[0004] The purpose of the present application is to provide a citrus double-factor anti-allergic plant extract and a preparation method and application thereof. The citrus double-factor anti-allergic plant extract has excellent moisturizing and soothing effects, and can effectively promote the popularization of cosmetics.

[0005] A citrus double-factor anti-allergic plant extract, which is composed of aurantiamarin and naringin, and the mass ratio of the aurantiamarin and the naringin is (20-25):(6-10).

[0006] Optionally, the mass ratio of the aurantiamarin and the naringin is 22:9.

[0007] A preparation method of the citrus double-factor anti-allergic plant extract, comprising the following steps: S1: raw material pretreatment: selecting fresh trifoliate orange fruits, removing the kernels after washing, and crushing the pulp and peel into trifoliate orange fruit particles with a particle size of 2-5 mm; S2: heating reflux extraction: adding the crushed trifoliate orange fruit particles to an ethanol solution with a volume concentration of 50-80% according to a solid-liquid ratio of 1g:8mL-1g:12mL, heating reflux extraction at 70-90℃ for 5-8h, and filtering to obtain the filtrate as the extraction crude product; S3: Macroporous resin pretreatment: AB-8 type macroporous adsorption resin was selected, and 95% (by volume) ethanol was soaked for 24 h. After column loading, 95% (by volume) ethanol and distilled water were used to rinse the column until no alcohol smell was detected; S4: Column loading and adsorption: The crude product was loaded onto the column at a flow rate of 1-2 BV / h, and BV was the column volume; S5: Water washing to remove impurities: The resin column was washed with 3-5 BV of distilled water at a flow rate of 1-2 BV / h to remove water-soluble impurities; S6: Naringin elution and purification: 2-3 BV of 30-40% (by volume) ethanol solution was used to elute at a flow rate of 1-2 BV / h. The eluate was concentrated, recrystallized, and dried to obtain naringin; S7: Naringin elution and purification: 2-3 BV of 50-70% (by volume) ethanol solution was used to elute at a flow rate of 1-2 BV / h. The eluate was concentrated, recrystallized, and dried to obtain naringin; S8: Compound: Naringin and naringin were mixed in a mass ratio of (20-25):(6-10), deionized water was added, stirred and dissolved, and then the solution was spray dried at an inlet temperature of 180-200℃ and an outlet temperature of 80-90℃ to obtain the citrus double-factor anti-sensitivity plant extract.

[0008] Optionally, in step S2, stirring was performed for 10-15 min every 1 h during the heating reflux extraction process.

[0009] Optionally, in step S3, the flow rate of 95% (by volume) ethanol used to rinse the resin column was 2-3 BV / h, and the eluate was consistent with the ethanol blank at 280 nm.

[0010] Optionally, in step S6, the concentration conditions of naringin eluate were 50-60℃ and a vacuum degree of 0.06-0.08 MPa, and the relative density at 25℃ was 1.10-1.15 after concentration. Distilled water was added at 5-8 times the volume of naringin eluate for recrystallization, heated to 80-90℃ for dissolution, and cooled to 4-8℃ for crystallization for 12-24 h.

[0011] Optionally, in step S7, the concentration conditions of naringin eluate were 50-60℃ and a vacuum degree of 0.06-0.08 MPa, and the relative density at 25℃ was 1.20-1.25 after concentration. Distilled water was added at 4-6 times the volume of naringin eluate for recrystallization, heated to 85-95℃ for dissolution, and cooled to 4-8℃ for crystallization for 16-28 h.

[0012] Optionally, in step S8, the amount of deionized water added was 5-10 times the total mass of naringin and naringin, the stirring temperature was 40-50℃, and the stirring time was 30-60 min.

[0013] A cosmetic product comprising the citrus double-factor anti-sensitivity plant extract, the citrus double-factor anti-sensitivity plant extract accounting for 0.5-5% by mass of the cosmetic product.

[0014] Optionally, the cosmetic product is a lotion, a cream, an essence, a toner, or a mask.

[0015] Beneficial effects In the present application, when naringin and naringin are compounded according to the mass ratio, they can produce significant synergistic effect, not only can respectively exert the anti-inflammatory and antioxidant effect of naringin and the moisturizing and soothing effect of naringin, but also can promote each other, further improve the overall anti-sensitivity effect and skin tolerance, and effectively improve the problems of skin sensitivity, redness, dryness, etc. DETAILED DESCRIPTION

[0016] An embodiment of the present application provides a citrus double-factor anti-sensitivity plant extract, which is composed of naringin and naringin, and the mass ratio of naringin to naringin is (20-25):(6-10). When naringin and naringin are compounded according to the mass ratio, they can produce significant synergistic effect, not only can respectively exert the anti-inflammatory and antioxidant effect of naringin and the moisturizing and soothing effect of naringin, but also can promote each other, further improve the overall anti-sensitivity effect and skin tolerance, and effectively improve the problems of skin sensitivity, redness, dryness, etc. The anti-inflammatory and antioxidant properties of naringin and the moisturizing and soothing effect of naringin are complementary, and when they are compounded in this ratio, the problem of insufficient moisturizing of naringin used alone is avoided, and the defect of weak anti-sensitivity ability of naringin used alone is solved, a synergistic and lasting anti-sensitivity and moisturizing effect is obtained, and the problems of skin sensitivity, redness, dryness, and water deficiency can be effectively improved.

[0017] Optionally, the mass ratio of naringin to naringin is 22:9. At this time, the citrus double-factor anti-sensitivity plant extract can obtain a more optimal moisturizing and soothing effect.

[0018] Optionally, the purity of naringin and naringin is greater than or equal to 98%. High-purity active ingredients can directly improve the efficacy stability and safety of the plant extract: on the one hand, the low impurity content can avoid the irritation of impurities to the skin, such as the impurities such as sugars and pigments in low-purity ingredients that can easily cause discomfort in sensitive skin, fully embodying the advantages of high purity. On the other hand, high-purity active ingredients ensure that the actual content of active ingredients is consistent when compounded, avoid the fluctuation of efficacy caused by impurity interference, provide protection for the precise control of efficacy in cosmetic production, and at the same time, high-purity ingredients also improve the industrial value of the plant extract, which can reduce the addition amount of other stabilizers and neutralizing agents in the subsequent cosmetic formula, and simplify the formula system.

[0019] An embodiment of the present application provides a preparation method of the above-mentioned citrus double-factor anti-sensitivity plant extract, comprising the following steps: S1: raw material pretreatment: select fresh trifoliate orange fruit, remove the core after washing, crush the fruit flesh and peel into trifoliate orange fruit particles with a particle size of 2-5 mm; S2: heating reflux extraction: add the crushed trifoliate orange fruit particles to ethanol solution with a volume concentration of 50-80% according to the solid-liquid ratio of 1 g:8 mL-1 g:12 mL, heat reflux extraction at 70-90°C for 5-8 h, filter, and take the filtrate as the extraction crude product; S3: macroporous resin pretreatment: select AB-8 type macroporous adsorption resin, soak in ethanol with a volume concentration of 95% for 24 h, and then wash with ethanol with a volume concentration of 95% and distilled water in sequence until there is no alcohol smell after column loading; S4: column loading and adsorption: load the extraction crude product on the column at a flow rate of 1-2 BV / h, where BV is the column volume; S5: water washing to remove impurities: wash the resin column with 3-5 BV of distilled water at a flow rate of 1-2 BV / h to remove water-soluble impurities; S6: naringin elution and purification: elute with 2-3 BV of ethanol solution with a volume concentration of 30-40% at a flow rate of 1-2 BV / h, concentrate the eluate, recrystallize, and dry to obtain naringin; S7: poncirin elution and purification: elute with 2-3 BV of ethanol solution with a volume concentration of 50-70% at a flow rate of 1-2 BV / h, concentrate the eluate, recrystallize, and dry to obtain poncirin; S8: compounding: mix naringin and poncirin according to the mass ratio (20-25):(6-10), add deionized water to stir and dissolve, and then spray dry the solution at an inlet air temperature of 180-200°C and an outlet air temperature of 80-90°C to obtain the citrus double-factor anti-sensitivity plant extract.

[0020] In the above preparation method, the whole process of raw material pretreatment, extraction, purification, and compounding is covered, and has the following core advantages: first, the process has strong compatibility and close connection between steps, the extraction crude product is directly suitable for column loading of macroporous resin without complex pretreatment, and continuous production can be realized, which is suitable for industrialization promotion. Second, the extraction process has strong pertinence and can efficiently dissolve naringin and poncirin, reducing production cost. Third, the macroporous resin gradient elution process (30-40% ethanol elution of naringin and 50-70% ethanol elution of poncirin) realizes efficient separation of the two components, solving the problem of difficult separation and low purity of the two components in traditional processes. Fourth, spray drying can quickly convert the liquid plant extract into powder, and the powder plant extract has a longer shelf life, is easier to store and transport, and at the same time widens the application scenarios. Fifth, the overall process is environmentally friendly and controllable, ethanol can be recycled, and distilled water washing and low-temperature drying reduce energy consumption and pollution, in line with the concept of green production.

[0021] In some embodiments, in step S2, the heating reflux extraction process is stirred for 10-15 min every 1 h. The stirring can break the concentration gradient of the extraction system, avoid the formation of a concentration barrier around the citrus fruit particles, enable the ethanol solution to penetrate more fully into the raw material, accelerate the dissolution of naringin and naringin, and increase the extraction rate of the target components by 15-20% compared to the extraction process without stirring; at the same time, the timed stirring can ensure that the temperature and concentration in each area of the extraction tank are consistent, avoid local overheating leading to degradation of the components, ensure the stability of the active ingredients in the crude product, and provide a high-quality raw material basis for the subsequent purification steps.

[0022] In some embodiments, in step S3, the flow rate of 95% ethanol for flushing the resin column is 2-3 BV / h until the effluent has an absorbance at 280 nm consistent with the ethanol blank. This can ensure the adsorption performance and purification effect of the resin: a flow rate of 2-3 BV / h can ensure that the ethanol fully contacts the resin pores and completely removes impurities such as monomers and oligomers remaining in the resin production process. The presence of these impurities can be adsorbed on the surface of the resin, occupy the adsorption sites, and reduce the adsorption capacity of naringin and naringin. A flow rate that is too fast can also result in insufficient flushing. The criterion of "effluent absorbance at 280 nm consistent with the ethanol blank" provides a precise quantitative basis for the pretreatment effect of the resin, avoids the problem of incomplete pretreatment caused by subjective judgment, ensures that the resin can efficiently and selectively adsorb the target components during subsequent column adsorption, and improves the purity of the purified product.

[0023] In some embodiments, in step S6, the concentration conditions for the naringin eluate are 50-60°C, a vacuum degree of 0.06-0.08 MPa, and a relative density of 1.10-1.15 at 25°C after concentration; and when recrystallizing, 5-8 times the volume of distilled water is added to the naringin eluate, heated to 80-90°C to dissolve, and then cooled at 4-8°C for 12-24 h to crystallize. Low-temperature vacuum concentration can avoid the oxidative degradation of naringin caused by high temperature, and quickly remove ethanol to reduce the loss of target components. Controlling the relative density (1.10-1.15) ensures that the concentration of naringin in the concentrated solution is appropriate, avoiding both low concentration leading to low crystallization efficiency and high concentration leading to impurities co-crystallizing. The amount of distilled water used during recrystallization (5-8 times), the dissolution temperature (80-90°C), and the crystallization conditions (4-8°C, 12-24 h) can form regular naringin crystals, effectively separate impurities, and ultimately obtain naringin with a purity of ≥98%.

[0024] In some embodiments, in step S7, the concentration conditions of the naringin eluent are 50-60℃, a vacuum degree of 0.06-0.08 MPa, and the concentration is performed to a relative density of 1.20-1.25 at 25℃; in the recrystallization, 4-6 times the volume of distilled water is added to the naringin eluent, heated to 85-95℃ for dissolution, and then cooled at 4-8℃ for 16-28h for crystallization. At this time, the concentration relative density (1.20-1.25), the amount of distilled water (4-6 times), the dissolution temperature (85-95℃), and the crystallization time (16-28h) ensure that the naringin can be fully dissolved and crystallized. For example, a higher dissolution temperature (85-95℃) is suitable for the lower water solubility of naringin, so as to avoid a decrease in yield due to insufficient dissolution; a longer crystallization time (16-28h) ensures that the naringin crystals grow fully, so as to reduce the impurities wrapped in the crystals, and finally obtain naringin with a purity of ≥98%, which is synergistic with the purification process of naringin, and realizes efficient separation and high-purity acquisition of the two components.

[0025] In some embodiments, in step S8, the amount of deionized water added is 5-10 times the total mass of naringin and naringin, the stirring temperature is 40-50℃, and the stirring time is 30-60min. In this way, it can be ensured that the naringin and naringin can be fully dissolved and uniformly mixed, and the local concentration is not too high to cause uneven distribution of components in the subsequent cosmetic formulation.

[0026] An embodiment of the present application provides a cosmetic, which comprises the above-mentioned citrus double-factor anti-sensitivity plant extract, and the mass percentage of the citrus double-factor anti-sensitivity plant extract in the cosmetic is 0.5-5%. The minimum addition amount of 0.5% can ensure that the cosmetic has basic moisturizing and anti-sensitivity effects, and avoid the case that the effects are not obvious due to too low addition amount. The maximum addition amount of 5.0% avoids the case that the skin is burdened due to too much active ingredient. At the same time, the addition amount range has wide formula compatibility, can adapt to the formula requirements of different dosage forms of cosmetics, does not need to greatly adjust the proportion of other ingredients, reduces the formula development cost of cosmetic production enterprises, and promotes the industrialization application of the plant extract.

[0027] Optionally, the cosmetic is a lotion, a cream, an essence, a toner, or a mask.

[0028] It can be understood that the cosmetic further comprises acceptable adjuvants.

[0029] Embodiment 1 The preparation method of the citrus double-factor anti-sensitivity plant extract in the present embodiment is as follows: S1: raw material pretreatment: 1kg of fresh poncirus fruit is selected, the fruit core is removed after washing, and the fruit pulp and peel are crushed into poncirus fruit particles with a particle size of 3mm.

[0030] S2: heating reflux extraction: the crushed fruit particles of Citrus aurantium L. were added into 60% ethanol solution according to the ratio of 1g:10mL, and heated at 80℃ for 6h. The solution was stirred every 1h for 12min. The filtrate was obtained by hot filtration, and served as the extraction crude product.

[0031] S3: macroporous resin pretreatment: 500g AB-8 macroporous adsorption resin was soaked in 95% ethanol solution for 24h, and stirred every 6h. The soaked resin was loaded into a chromatographic column (the ratio of column diameter to column height was 1:8), and then washed with 95% ethanol solution at a flow rate of 2.5BV / h until the absorbance of the effluent at 280nm was consistent with the ethanol blank. Distilled water was used to wash the column at a flow rate of 2.5BV / h until there was no alcohol smell, and the column was ready for use.

[0032] S4: column adsorption: the extraction crude product was cooled to room temperature, and then loaded into the column at a flow rate of 1.5BV / h, and passed through the AB-8 macroporous resin column.

[0033] S5: water washing to remove impurities: the resin column was washed with 4BV of distilled water at a flow rate of 1.5BV / h to remove water-soluble impurities.

[0034] S6: naringin elution and purification: 2.5BV of 35% ethanol solution was used to elute the resin column at a flow rate of 1.5BV / h, and the naringin eluate was collected. The eluate was concentrated under the condition of 55℃ and vacuum degree of 0.07MPa to a relative density of 1.12 (25℃). 6 times the volume of distilled water was added to the concentrated solution, heated to 85℃ for dissolution, and then naturally cooled to room temperature. The solution was placed in a 6℃ refrigerator for crystallization for 18h. The crystals were collected by filtration, washed twice with ice-cold distilled water, and then dried in a vacuum drying oven at 65℃ for 5h to obtain naringin with a purity of 98.7%.

[0035] S7: poncirin elution and purification: after the naringin elution was completed, 2.5BV of 60% ethanol solution was used to elute the resin column at a flow rate of 1.5BV / h, and the poncirin eluate was collected. The eluate was concentrated under the condition of 55℃ and vacuum degree of 0.07MPa to a relative density of 1.22 (25℃). 5 times the volume of distilled water was added to the concentrated solution, heated to 90℃ for dissolution, and then naturally cooled to room temperature. The solution was placed in a 6℃ refrigerator for crystallization for 22h. The crystals were collected by filtration, washed twice with ice-cold distilled water, and then dried in a vacuum drying oven at 65℃ for 6h to obtain poncirin with a purity of 98.5%.

[0036] S8: compounding: mixing naringin and poncirin at a mass ratio of 25:6, adding deionized water to stir and dissolve, the amount of deionized water added is 8 times the total mass of poncirin and naringin, stirring at 45℃ for 45min, then spray drying the solution at an inlet temperature of 180℃ and an outlet temperature of 80℃ to obtain citrus double-factor anti-sensitivity plant extract.

[0037] Example 2 Compared with Example 1, the difference of Example 2 is that in S8, naringin and poncirin are mixed at a mass ratio of 20:10.

[0038] Example 3 Compared with Example 1, the difference of Example 3 is that in S8, naringin and poncirin are mixed at a mass ratio of 22:9.

[0039] Comparative Example 1 Compared with Example 1, the difference of Comparative Example 1 is that in S8, naringin and poncirin are mixed at a mass ratio of 15:10.

[0040] Comparative Example 2 Compared with Example 1, the difference of Comparative Example 2 is that in S8, naringin and poncirin are mixed at a mass ratio of 30:6.

[0041] Comparative Example 3 In Comparative Example 3, the preparation method of the plant extract is as follows: S1: raw material pretreatment: select fresh poncirus fruit, remove the core after washing, crush the pulp and peel into poncirus fruit particles with a particle size of 3mm.

[0042] S2: heating reflux extraction: add the crushed poncirus fruit particles into the extraction tank, add distilled water according to the solid-liquid ratio of 1g:10mL, heat reflux extraction at 95℃ for 8h, filter the obtained filtrate as the extraction crude product.

[0043] S3: spray drying the extraction crude product at an inlet temperature of 180℃ and an outlet temperature of 80℃ to obtain the plant extract.

[0044] Test Example (1) Moisturizing performance: According to QB / T 4256-2011 Guide for Evaluation of Moisturizing Effect of Cosmetics, 30 healthy volunteers (20-40 years old, skin type is neutral) were selected, and after being adapted in the environment of temperature 25±2℃ and relative humidity 50±5% for 30 min, multiple 2cm x 2cm test areas were selected on the inner side of the left forearm, and each test area was coated with the emulsion containing the plant extract of the example or the comparative example (the mass percentage of the plant extract in the emulsion was 2%), and the skin water content of the stratum corneum was tested by using a skin moisture tester before coating, 0.5h, 1h, 2h and 4h after coating respectively, and the moisturizing rate was calculated. The moisturizing rate = (moisture content after coating - moisture content before coating) / moisture content before coating x 100%. The results are shown in Table 1.

[0045] (2) Soothing performance: 30 sensitive skin volunteers (20-40 years old, with sensitive symptoms such as skin redness and itching) were selected, and after being adapted in the environment of temperature 25±2℃ and relative humidity 50±5% for 30 min, the essence containing the plant extract of the example or the comparative example was coated on the red area of the face (the mass percentage of the plant extract in the essence was 2%), and the coating was performed once a day in the morning and evening, and the continuous use was 7 days, and the a Values (in millimeters) value of the red area was tested by using a skin colorimeter before use, 3 days and 7 days after use respectively (the larger the a value, the more serious the redness), and the redness subsidence rate was calculated. The redness subsidence rate = (a value before use - a value after use) / a value before use x 100%). At the same time, the skin irritation reaction (such as tingling, itching and burning sensation) of the volunteers during use was recorded, and the irritation rate was calculated. The irritation rate = the number of volunteers with irritation reaction / the total number of volunteers x 100%. The results are shown in Table 1.

[0046] Table 1

[0047] It can be seen from the above table that the plant extract in the example can be used in cosmetics, and the cosmetics have good moisturizing and soothing effects.

[0048] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

[0049] For those skilled in the art, according to the idea of the present application, there will be changes in specific embodiments and application scope, and on the basis of the above, the content of the specification should not be understood as a limitation of the present application.

Claims

1. A citrus dual-factor anti-allergy plant extract, characterized in that, It is composed of citric acid and naringin, wherein the mass ratio of citric acid to naringin is (20-25):(6-10).

2. The citrus dual-factor anti-allergy plant extract as described in claim 1, characterized in that, The mass ratio of citric acid to naringin is 22:

9.

3. A method for preparing a citrus dual-factor anti-allergy plant extract as described in any one of claims 1-2, characterized in that, Includes the following steps: S1: Raw material pretreatment: Select fresh goji berries, wash them and remove the seeds, then crush the pulp and peel into goji berry particles with a particle size of 2-5mm. S2: Heating and reflux extraction: Add the crushed tangerine fruit particles to an ethanol solution with a volume concentration of 50-80% at a material-to-liquid ratio of 1g:8mL-1g:12mL, heat and reflux at 70-90℃ for 5-8h, filter, and use the filtrate as the crude extract. S3: Macroporous resin pretreatment: Select AB-8 type macroporous adsorption resin, soak it in 95% ethanol for 24 hours, and after packing the column, rinse it with 95% ethanol and distilled water in sequence until there is no alcohol odor. S4: Column adsorption: The crude product is loaded onto the column at a flow rate of 1-2 BV / h, where BV is the column volume; S5: Water washing to remove impurities: Rinse the resin column with 3-5 BV of distilled water at a flow rate of 1-2 BV / h to remove water-soluble impurities; S6: Naringin elution and purification: Elute with 2-3 BV of 30-40% ethanol solution at a flow rate of 1-2 BV / h, concentrate the eluent, recrystallize and dry to obtain naringin; S7: Purification of citric acid by elution: Elute with 2-3 BV of 50-70% ethanol solution at a flow rate of 1-2 BV / h. Concentrate the eluent, recrystallize, and dry to obtain citric acid. S8: Compound preparation: Naringin and limonene are mixed at a mass ratio of (20-25):(6-10), deionized water is added and stirred to dissolve, and then the solution is spray-dried at an inlet air temperature of 180-200℃ and an outlet air temperature of 80-90℃ to obtain the citrus dual-factor anti-allergy plant extract.

4. The preparation method of the citrus dual-factor anti-allergy plant extract as described in claim 3, characterized in that, In step S2, the mixture is stirred for 10-15 minutes every hour during the heating and reflux extraction process.

5. The preparation method of the citrus dual-factor anti-allergy plant extract as described in claim 3, characterized in that, In step S3, the resin column is rinsed with 95% ethanol at a flow rate of 2-3 BV / h until the absorbance of the effluent at 280 nm is consistent with that of the ethanol blank.

6. The preparation method of the citrus dual-factor anti-allergy plant extract as described in claim 3, characterized in that, In step S6, the concentration conditions of the naringin eluent are 50-60℃ and vacuum degree 0.06-0.08MPa, and the relative density at 25℃ is 1.10-1.

15. During recrystallization, 5-8 times the volume of distilled water of the naringin eluent is added, heated to 80-90℃ to dissolve, and then refrigerated at 4-8℃ for 12-24 hours for crystallization.

7. The preparation method of the citrus dual-factor anti-allergy plant extract as described in claim 3, characterized in that, In step S7, the concentration conditions of the citric acid eluent are 50-60℃ and vacuum degree 0.06-0.08MPa, and the relative density at 25℃ is 1.20-1.

25. During recrystallization, 4-6 times the volume of distilled water of the citric acid eluent is added, heated to 85-95℃ to dissolve, and then refrigerated at 4-8℃ for 16-28 hours for crystallization.

8. The method for preparing the citrus dual-factor anti-allergy plant extract as described in any one of claims 3-7, characterized in that, In step S8, the amount of deionized water added is 5-10 times the total mass of citric acid and naringin, the stirring temperature is 40-50℃, and the stirring time is 30-60 min.

9. A cosmetic product, characterized in that, The product includes any one of the citrus dual-factor anti-allergy plant extracts according to claims 1-2, wherein the citrus dual-factor anti-allergy plant extracts account for 0.5-5% of the mass of the cosmetic product.

10. The cosmetic product as described in claim 9, characterized in that, The cosmetics mentioned are lotions, creams, serums, toners, or masks.

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