Greenhouse vegetable organic coupling cultivation method and application

Abstract

The invention provides a greenhouse vegetable organic coupling cultivation method and application. The method comprises the steps that square ditches are dug one month before greenhouse vegetable seedlings are transplanted, corn straw, Penicillium cyclopium 455-1 bacterial suspension, viral vaccines, peanut cakes, wheat bran, farmyard manure, bacterial manure, compound fertilizer and water are sequentially added, then the square ditches are flatly covered with soil and are covered and sealed with a plastic film, and then the greenhouse vegetable seedlings are transplanted. After one month, punching holes in the surface of the mulching film to transplant vegetable seedlings. According to the greenhouse vegetable 455-1 mutant strain fermentation liquor, corn straw and organic fertilizer organic coupling cultivation technology, the growth vigor, root development and photosynthetic efficiency of greenhouse tomatoes, peppers and cucumbers and the resistance to soil-borne oomycetes diseases are remarkably improved.

Description

Technical Field

[0001] The invention relates to the technical field of facility vegetable cultivation, in particular to a facility vegetable organic coupling cultivation method and application. Background Art

[0002] Oomycete diseases of greenhouse vegetables seriously restrict the safe production and benefits of greenhouse vegetables. Oomycete diseases of greenhouse vegetables mainly include airborne oomycete diseases and soil-borne oomycete diseases. Soil-borne oomycete diseases of greenhouse vegetables are important soil-borne root and stem diseases of greenhouse vegetables, mainly including cucumber, tomato, pepper, eggplant blight, cotton rot and damping-off, which seriously affect the healthy development of the greenhouse vegetable industry. At present, the prevention and control technology of soil-borne oomycete diseases of greenhouse vegetables mainly relies on chemical pesticides, single pesticide use, excessive pesticide use and random pesticide use, which makes it difficult to effectively prevent and control the occurrence and harm of soil-borne oomycete diseases of greenhouse vegetables. In recent years, the cultivation technology of fertilizer-water coupling, substrate fertilization coupling and sub-membrane drip irrigation water-fertilizer coupling has played an important role in enhancing the growth of greenhouse vegetables, improving quality, increasing the yield of organic vegetables, enhancing resistance to soil-borne diseases and reducing the use of chemical fertilizers and pesticides, and has gradually become a new type of organic cultivation disease control technology.

[0003] Establish and improve new organic cultivation and disease control technologies for facility vegetables, reduce the use of pesticides and fertilizers, effectively control the occurrence and harm of soil-borne oomycete diseases, and ensure the increase in green organic vegetable yields and safe production. Substrate coupling technology is the main organic coupling cultivation technology for facility vegetables. Research and development and improvement of the matrix organic coupling cultivation technology for facility vegetables is expected to become a new organic cultivation technology that effectively promotes the growth of facility vegetables, enhances disease resistance, and effectively controls the occurrence and harm of soil-borne oomycete diseases of facility vegetables. The matrix coupling cultivation technology is to mix straw and organic fertilizer or bacterial fertilizer in deep trenches about one month before vegetable transplanting. This technology is low-cost, easy to operate, and easy to promote and utilize. However, it mainly relies on the deep trench soil ecological environment to degrade the straw matrix. It is difficult to effectively degrade and utilize the organic components of the straw in a short period of time, which can easily lead to a large amount of uncomposted straw matrix remaining in the soil matrix, reducing the technical performance and effect of matrix coupling cultivation.

[0004] The biodegradation of lignocellulose in crop straw mainly depends on the cellulase, hemicellulase and ligninase secreted by the degradable microorganisms themselves and their activities. The degradation of carbohydrates such as cellulose and hemicellulose will produce sugars. The changes in the total sugar and reducing sugar content during the biodegradation of crop straw reflect the degradation of carbohydrates. Therefore, during the biodegradation of crop straw, the determination of various lignocellulase activities and the changes in the total sugar and reducing sugar content are important technical parameters reflecting the degree of straw degradation. Therefore, it provides a reference for the isolation and identification of microbial strains with the ability to degrade crop straw from crop straw and its improvement facilities for the organic coupling cultivation technology of vegetable straw-fertilizer. Summary of the invention

[0005] The purpose of the invention is to provide a method for organically coupling cultivation of facility vegetables and its application.

[0006] The present invention isolates a Penicillium strain 2554-2 (i.e., strain MP-03) from corn stalks that improves the cellulase activity of corn stalks, and is identified as Penicillium circumflexus ( Penicillium cyclopium ) The strain 2554-2 was induced to mutate using the nitrosoguanidine method, and the mutant strain 455-1 (i.e., strain ΔMP-03) with strong corn straw degradation was obtained.

[0007] Penicillium arcus ( Penicillium cyclopium ) The 455-1 strain has been deposited in the General Microbiology Center of China Microbiological Culture Collection (CGMCC), No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100101, China. The deposit number is CGMCC NO.41541, the deposit date is October 12, 2024, and the proposed taxonomic name is Penicillium circumflexus ( Penicillium cyclopium ) .

[0008] Strain 2554-2 has also been deposited in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Administration, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, 100101, China, with the deposit number CGMCC NO.41542 and the deposit date October 12, 2024. The proposed taxonomic name is Penicillium circumflexum ( Penicillium cyclopium ) .

[0009] In a first aspect, the present invention provides a method for organically coupled cultivation of facility vegetables, the method comprising: digging a square ditch one month before transplanting the facility vegetable seedlings, adding the following components in sequence, corn stalks - arc Penicillium 455-1 suspension - virus vaccine - peanut cake - wheat bran - farmyard manure - bacterial fertilizer - compound fertilizer - water, then covering it evenly with soil and sealing it with a mulch film, and after one month, drilling holes on the surface of the mulch film to transplant the vegetable seedlings.

[0010] Furthermore, in terms of weight percentage, the addition ratio of each component is as follows: corn straw (crushed or not crushed) 50%, peanut cake 8.2%, wheat bran 8.2%, farmyard manure 9.7% (the main ingredients include organic matter, nitrogen, phosphorus, potassium and other nutrients, purchased from Shandong Green Protection Biotechnology Co., Ltd.), bacterial fertilizer 6.8% (Jinzhengda Soil-Friendly No. 1), compound fertilizer 5.2% (nitrogen, phosphorus and potassium content 45%-55%, purchased from Anyang Zhongsheng Fertilizer Co., Ltd.), water 6.8%, arc Penicillium 455-1 bacterial suspension (200 million spores / mL) 5%, vegetable virus vaccine (attenuated strain vaccine or double-linked small RNA vaccine, mainly used to prevent and control the occurrence and harm of vegetable viruses in the later stage, purchased from Nanjing Ruimate Biotechnology Co., Ltd.) 0.1%.

[0011] The straws were incubated at 30°C for 7 days and the weight loss rate of the straws and the changes in the cellulase activity were measured. A non-mutant arc Penicillium suspension was used as a control with an inoculation rate of 5% (200 million spores / mL).

[0012] In the present invention, the vegetables include but are not limited to tomatoes, peppers and cucumbers.

[0013] In a second aspect, the method of the present invention is used in preventing and controlling soil-borne diseases of tomatoes, peppers and cucumbers.

[0014] Such diseases include, but are not limited to, tomato late blight caused by Phytophthora infestans, cucumber blight caused by Phytophthora drechsleri, and pepper blight caused by Phytophthora capsici.

[0015] The organic coupled cultivation technology of greenhouse vegetable 455-1 mutant fermentation liquid+corn stalks+organic fertilizer provided by the present invention significantly improves the growth, root development and photosynthetic efficiency of greenhouse tomatoes, peppers and cucumbers, as well as the resistance to soil-borne oomycete diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of tomato stem height.

[0017] Figure 2 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of pepper stem height.

[0018] Figure 3 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of cucumber stem height.

[0019] Figure 4 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of tomato stem thickness.

[0020] Figure 5 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of cucumber stem thickness.

[0021] Figure 6 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the growth of pepper stem thickness.

[0022] Figure 7 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the vitality of tomato roots.

[0023] Figure 8 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the root activity of pepper.

[0024] Fig. 9 In the preferred embodiment of the present invention, ΔMP-03 organic coupling cultivation promotes the root activity of cucumber.

[0025] Fig.10 This is the effect of ΔMP-03 organic coupled cultivation on the dry weight of tomato roots in a preferred embodiment of the present invention.

[0026] Fig.11 This is the effect of ΔMP-03 organic coupled cultivation on the dry weight of cucumber roots in a preferred embodiment of the present invention.

[0027] Fig.12 This is the effect of ΔMP-03 organic coupled cultivation on the dry weight of pepper roots in a preferred embodiment of the present invention.

[0028] Fig.13 This is the effect of ΔMP-03 organic coupled cultivation on tomato leaf area in a preferred embodiment of the present invention.

[0029] Fig.14 This is the effect of ΔMP-03 organic coupled cultivation on cucumber leaf area in a preferred embodiment of the present invention.

[0030] Fig.15 This is the effect of ΔMP-03 organic coupled cultivation on pepper leaf area in a preferred embodiment of the present invention.

[0031] Fig.16 This is the effect of ΔMP-03 organic coupled cultivation on the chlorophyll content of tomato leaves in a preferred embodiment of the present invention.

[0032] Fig.17 This is the effect of ΔMP-03 organic coupled cultivation on the chlorophyll content of cucumber leaves in a preferred embodiment of the present invention.

[0033] Fig.18This is the effect of ΔMP-03 organic coupled cultivation on the chlorophyll content of pepper leaves in a preferred embodiment of the present invention.

[0034] Fig.19 The ΔMP-03 organic coupling cultivation technology in the preferred embodiment of the present invention significantly reduces the disease index of tomato late blight.

[0035] Fig. 20 The ΔMP-03 organic coupling cultivation technology in the preferred embodiment of the present invention significantly reduces the disease index of cucumber blight.

[0036] Fig.21 The ΔMP-03 organic coupling cultivation technology in the preferred embodiment of the present invention significantly reduces the disease index of pepper blight. DETAILED DESCRIPTION

[0037] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0038] The data processing method in the following examples uses Office 2003 software Excel for data processing, tabulation and drawing, and PPSS 18.0 statistical software is used for data significance analysis.

[0039] Example 1 Penicillium mutant strain 455-1 degrades corn stalks and organic matrix coupled cultivation technology

[0040] 1. Apply Penicillium citrinum 455-1 suspension, which has a significant ability to degrade corn stalks, to establish an improved organic matrix coupled cultivation technology for greenhouse vegetables

[0041] One month before transplanting the greenhouse vegetable seedlings, the dimensions of the square trenches should be: 35cm deep, 60cm wide, and 8.2m long. About 30-35 square trenches should be dug per acre.

[0042] Proportion of adding corn straw and other ingredients in a single trench:

[0043] Calculated by weight percentage, the addition ratio of each component is as follows: corn straw (crushed or not crushed) 50%, peanut cake 8.2%, wheat bran 8.2%, farmyard manure 9.7% (the main ingredients include organic matter, nitrogen, phosphorus, potassium and other nutrients, purchased from Shandong Green Protection Biotechnology Co., Ltd.), bacterial fertilizer 6.8% (Jinzhengda Soil-Friendly No. 1), compound fertilizer 5.2% (nitrogen, phosphorus and potassium content 45%-55%, purchased from Anyang Zhongsheng Fertilizer Co., Ltd.), water 6.8%, Arc Penicillium 455-1 bacterial suspension (200 million spores / mL) 5%, vegetable virus vaccine (attenuated strain vaccine or double-linked small RNA vaccine, mainly used to prevent and control the occurrence and harm of vegetable viruses in the later stage, purchased from Nanjing Ruimate Biotechnology Co., Ltd.) 0.1%.

[0044] The straws were incubated at 30°C for 7 days and the weight loss rate of the straws and the changes in the cellulase activity were measured. A non-mutant arc Penicillium suspension was used as a control with an inoculation rate of 5% (200 million spores / mL).

[0045] One month before transplanting vegetable seedlings in the facility, the order of adding components to the dug square trenches is: evenly spreading corn straw - mutant or non-mutant Penicillium suspension - virus vaccine - peanut cake - wheat bran - farmyard manure - bacterial fertilizer - compound fertilizer - water in sequence (liquid components are evenly sprayed, solid or powder is evenly spread), then cover with soil and seal with mulch film. After one month, holes are punched on the surface of the film and transplant vegetable seedlings.

[0046] 2. Determination of multiple technical indicators of tomato, pepper and cucumber cultivation in organic coupling facilities by adding 455-1 bacterial suspension to degrade corn straw

[0047] 2.1 Improved organic coupled cultivation of tomato, pepper and cucumber plant height and stem diameter

[0048] 1) Plant height: The plant heights of tomatoes, peppers and cucumbers were measured at the seedling, flowering, fruiting and peak fruiting stages. Sampling was performed at 5 points. The plant heights of 50 tomato, pepper and cucumber plants were measured at each point. The height of each plant was determined based on the highest plant height, and the average value was taken.

[0049] 2) Stem diameter: The stem diameter of tomatoes, peppers and cucumbers was measured at the seedling, flowering, fruiting and peak fruiting stages using a vernier caliper. Sampling was performed at 5 points and the stem diameters of 50 tomato, pepper and cucumber plants were measured at each point. The thickness of the stem of each plant was determined at the thickest part of the stem base and the average value was taken.

[0050] 2.2 Improved greenhouse vegetable organic coupling cultivation technology Tomato, pepper and cucumber root growth measurement

[0051] The root dry weight and root activity of tomato, pepper and cucumber plants were measured at the seedling stage, flowering stage, fruiting stage and fruiting stage after transplantation. The root activity was measured by TTC method (Li Hesheng, 2002), and the root dry weight was measured by drying and using a 1 / 10,000 balance.

[0052] 2.3 Improved greenhouse vegetable organic coupling cultivation technology Determination of chlorophyll content and leaf area of ​​tomato, pepper and cucumber leaves

[0053] The chlorophyll content and leaf area of ​​tomatoes, peppers and cucumbers were measured during the seedling stage, early flowering stage, fruiting stage and peak fruiting stage.

[0054] 1) Chlorophyll content: acetone ethanol mixed solution method.

[0055] 2) Leaf area: The length × width of leaves of tomatoes, peppers and cucumbers were measured at the seedling stage, flowering stage, fruiting stage and peak fruiting stage. Sampling was carried out at 5 points, and the leaf sizes of 50 tomatoes, peppers and cucumbers were measured at each point. The length × width of 25 leaves in the middle of each tomato, pepper and cucumber plant were measured and the average value was taken.

[0056] 3. Results and Analysis

[0057] 3.1 Effects of ΔMP-03 organic coupling cultivation technology on promoting stem height growth of greenhouse tomatoes, peppers and cucumbers

[0058] Figure 1 , Figure 2 and Figure 3 The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks was beneficial to increase the plant height growth of tomato, pepper and cucumber seedlings in the later stage of transplanting. The measurement data showed that this technology made the plant height of tomatoes, peppers and cucumbers gradually increase from the initial flowering stage to the peak fruiting stage, and the plant height reached the highest in the peak fruiting stage. In contrast, the plant height growth of pepper plants from the initial flowering stage to the peak fruiting stage was lower, which may be because the pepper plants are naturally shorter. Therefore, the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks significantly accelerated the growth rate of plant height of greenhouse tomato, pepper and cucumber seedlings in the later stage of transplanting, which is beneficial to the growth and development of greenhouse vegetables. Figure 1 , Figure 2 and Figure 3 Different asterisks in the figure represent the significant differences in plant height growth among three different vegetables, *P<0.05, **P<0.01, ***P<0.001.

[0059] 3.2 Effects of ΔMP-03 organic coupling cultivation technology on the growth of stem thickness of greenhouse tomatoes, cucumbers and peppers

[0060] Figure 4 , Figure 5 and Figure 6 The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn straw significantly increased the growth of stem base thickness of tomatoes, cucumbers and peppers during the initial flowering period, fruiting period and peak fruiting period. The stem base thickness of the three vegetables showed a trend of gradual increase, and the growth of stem base thickness of tomatoes, cucumbers and peppers was most obvious during the peak fruiting period. Compared with tomatoes and cucumbers, the growth of stem base thickness of peppers from the initial flowering period to the peak fruiting period was relatively small due to the characteristics of the pepper variety. Based on the relevant data, the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn straw significantly accelerated the growth rate of stem base thickness of greenhouse tomatoes, cucumbers and peppers from the initial flowering period to the peak fruiting period, which was beneficial to the growth and development of greenhouse tomatoes, cucumbers and peppers and improved their resistance to soil-borne diseases. Figure 4 , Figure 5 and Figure 6 The different asterisks on the bar graph represent the significant differences in the thickness of the stem base of the three vegetables, **P<0.01, ***P<0.001.

[0061] 3.3 Effects of ΔMP-03 organic coupling cultivation technology on root activity of greenhouse tomatoes, peppers and cucumbers

[0062] Figure 7 , Figure 8 and Fig. 9 The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupled cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks significantly improved the root activity of tomatoes, peppers and cucumbers from the initial flowering stage, fruiting stage, and peak fruiting stage. The root activity of the three vegetables showed an increasing trend from the initial flowering stage to the peak fruiting stage, and the root activity reached the highest level in the peak fruiting stage. Based on the relevant data, the organic coupled cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks is conducive to enhancing the root growth and development of greenhouse tomatoes, cucumbers and peppers, significantly enhancing the growth of vegetables and improving the disease resistance to soil-borne fungal and oomycete diseases.

[0063] Figure 7 , Figure 8 and Fig. 9 Different asterisks on the bar graph represent significant differences in root activity, *P<0.05, **P<0.01, ***P<0.001.

[0064] 3.4 Effects of ΔMP-03 organic coupling cultivation technology on the dry weight of greenhouse tomatoes, peppers and tomato roots

[0065] Fig.10 , Fig.11 and Fig.12The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks significantly increased the root dry weight of tomatoes, peppers, and cucumbers from the initial flowering stage to the peak fruiting stage. The root dry weight of the three vegetables tested showed a gradual trend from the initial flowering stage, and the increase in root dry weight of tomatoes, peppers, and cucumbers was most obvious during the peak fruiting stage. Based on the relevant data, the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn stalks is conducive to increasing the root growth and development of greenhouse tomatoes, cucumbers, and peppers, and significantly enhancing the growth of greenhouse vegetables.

[0066] Fig.10 , Fig.11 and Fig.12 Each experiment was sampled at 5 points and the data were analyzed three times. The dry weight of at least 60 roots of each vegetable was measured each time. The different asterisks on the bar graph represent the significant differences in the dry weight of roots of the three vegetables, *P<0.05, **P<0.01, ***P<0.001.

[0067] 3.5 Effects of ΔMP-03 organic coupling cultivation technology on leaf area changes of greenhouse tomatoes, peppers and cucumbers

[0068] Fig.13 , Fig.14 and Fig.15 The results showed that compared with MP-03 and WT (without bacterial suspension), adding ΔMP-03 bacterial suspension to degrade corn straw organic coupling cultivation technology significantly increased the leaf area of ​​greenhouse tomatoes, cucumbers and peppers during the initial flowering period, fruiting period and peak fruiting period, and showed a gradually increasing trend, and the leaf area reached the maximum during the peak fruiting period. Based on the relevant data, adding ΔMP-03 bacterial suspension to degrade corn straw organic coupling cultivation technology significantly increased the growth of greenhouse tomatoes, cucumbers and peppers leaf area, which is beneficial to improve the photosynthetic efficiency and light energy utilization rate of tomato, cucumber and pepper leaves.

[0069] Fig.13 , Fig.14 and Fig.15 Sampling was carried out at 5 points in each experiment, and at least 50 leaves were taken from each point for each vegetable. The area measurement was repeated 3 times. The different asterisks on the bar graph represent the significant difference in the change of leaf area size of each vegetable, *P<0.05, **P<0.01, ***P<0.001.

[0070] 3.6 Effects of ΔMP-03 organic coupling cultivation technology on chlorophyll content in greenhouse tomatoes, peppers and cucumber leaves

[0071] Fig.16 , Fig.17 and Fig.18The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn straw significantly increased the chlorophyll content of tomato leaves during the initial flowering, fruiting and peak fruiting stages. The chlorophyll content of tomato leaves was the highest during the fruiting stage and began to decline during the peak fruiting stage. The chlorophyll content of pepper and cucumber leaves showed a gradual increasing trend from the initial flowering, fruiting and peak fruiting stages, and the content was the highest during the peak fruiting stage. Based on the relevant data, the organic coupling cultivation technology of adding ΔMP-03 bacterial suspension to degrade corn straw significantly increased the chlorophyll content of greenhouse tomatoes, cucumbers and peppers, which was beneficial to improving the photosynthetic efficiency of tomato, cucumber and pepper leaves.

[0072] Fig.16 , Fig.17 and Fig.18 Each experiment was repeated three times with sampling at 5 points and measurement. At least 50 leaves of each vegetable were measured each time. The different asterisks on the bar graph represent the significant difference in chlorophyll content of each vegetable leaf, *P<0.05, **P<0.01, ***P<0.001.

[0073] Example 2 Application of the improved organic coupling cultivation technology for greenhouse vegetables in preventing and controlling soil-borne diseases of tomatoes, peppers and cucumbers

[0074] 1. Investigation method of the damage caused by root and stem blight of tomatoes, peppers and cucumbers by improved organic coupling cultivation technology of greenhouse vegetables. The greenhouses of tomatoes, peppers and cucumbers treated with improved organic coupling cultivation technology of greenhouse vegetables and one control greenhouse were investigated starting from the sporadic outbreaks of soil-borne blight of tomatoes, peppers and cucumbers. The investigation was conducted once every 7 days, and samples were taken at 5 points on the diagonal each time. 50 plants were investigated at each point. The investigation was conducted 3 times in a row and the experiment was repeated 3 times.

[0075] 2. Improved organic coupled cultivation technology for greenhouse vegetables: classification standards for tomato, pepper and cucumber diseases and calculation of disease index

[0076] Level 0: No lesions on leaves or fruits;

[0077] Level 1: The lesion area accounts for less than 5% of the leaf area, the fruit lesions are watery light gray, accounting for 10-20% of the fruit area, and there are sporadic lesions on the stems;

[0078] Level 2: The lesion area accounts for 6%-10% of the leaf area, the fruit lesions are dark gray, accounting for 21%-30% of the fruit area, with sporadic mold layers and an increase in the number of lesions at the base of the stem;

[0079] Level 3: The lesion area accounts for 11%-20% of the leaf area, the fruit lesions are dark gray, accounting for 31%-40% of the fruit area, the mold layer accounts for 30%-40% of the lesions, and the lesions at the base of the stem are continuous, accounting for more than 20%-40% of the basic height of the stem;

[0080] Level 4: The lesion area accounts for 21%-50% of the leaf area, the fruit lesions are dark gray, accounting for 41%-50% of the fruit area, the mold layer accounts for 41%-50% of the lesions, and the lesions at the base of the stem account for 41%-50% of the basic height of the stem;

[0081] Level 5: The lesion area accounts for more than 50% of the leaf area, the mold layer accounts for more than 60% of the lesions, the fruit is rotten, and the lesions at the base of the stem account for more than 60% of the basic height of the stem;

[0082] Disease index = 100 × Σ (number of diseased leaves at each level × representative plants at each level) / (total number of leaves surveyed × highest representative value).

[0083] 3. Results and Analysis

[0084] The effects of ΔMP-03 organic coupling cultivation technology on the changes in the disease index of greenhouse tomatoes, peppers and cucumbers are as follows:

[0085] Fig.19 , Fig. 20 and Fig.21 The results showed that compared with MP-03 and WT (without bacterial suspension), the organic coupled cultivation technology of corn straw degradation with ΔMP-03 bacterial suspension significantly reduced the incidence and disease index of tomato late blight (caused by Phytophthora infestans), cucumber phytophthora (caused by Phytophthora drechsleri) and pepper phytophthora (caused by Phytophthora capsici) from seedling stage to fruiting stage in greenhouse tomatoes, cucumbers and peppers. Compared with the ΔMP-03 suspension treatment, the organic coupled cultivation technology of corn straw degradation with MP-03 and WT suspensions caused the disease index of soil-borne diseases of the three vegetables to gradually increase in the seedling stage, initial flowering stage, fruiting stage and fruiting stage, and the disease was the most serious in the fruiting stage. However, the organic coupled cultivation technology of corn straw degradation with ΔMP-03 suspension significantly reduced the incidence and disease index of soil-borne diseases of the three greenhouse vegetables at different developmental stages. Therefore, the organic coupling cultivation technology of ΔMP-03 suspension-degraded corn straw significantly inhibited the incidence of soil-borne diseases of greenhouse tomatoes, cucumbers and peppers, and significantly reduced the occurrence and harm of soil-borne diseases of tomatoes, cucumbers and peppers.

[0086] Fig.19 , Fig. 20 and Fig.21 Different asterisks on each bar graph represent the significant difference in the disease index of each vegetable soil-borne disease, *P<0.05, **P<0.01, ***P<0.001.

[0087] The comprehensive comparative analysis of the above results showed that compared with MP-03 and WT (without bacterial suspension added), in the conventional organic coupling cultivation technology, the addition of ΔMP-03 bacterial suspension to degrade corn straw organic coupling cultivation technology: 1) significantly increased the degradation rate of corn straw, resulting in a significant reduction in the weight of corn straw and a significant increase in the degradation rate of the three major components of lignocellulose; 2) it was determined that when the temperature was 30°C, the initial pH was 7.0, and farmyard manure or bacterial fertilizer was added, ΔMP-03 had a higher degradation effect on corn straw than MP-03; 3) it was determined that ΔMP-03 bacterial suspension had a stronger ability to activate the activity of corn straw endocellulase and exocellulase than MP-03, significantly accelerating the yield of corn straw fiber and the decomposition and utilization rate of corn straw; 4) ΔMP-0 3) The organic coupling cultivation technology of corn straw degradation by bacterial suspension increased the growth rate of plant height and stem diameter of greenhouse tomato, pepper and cucumber seedlings in the later stage of transplanting; 5) The organic coupling cultivation technology of corn straw degradation by ΔMP-03 bacterial suspension enhanced the root growth and development of greenhouse tomato, cucumber and pepper seedlings in the later stage of transplanting, which is beneficial to increase the growth and disease resistance of greenhouse tomato, cucumber and pepper; 6) The organic coupling cultivation technology of corn straw degradation by ΔMP-03 bacterial suspension significantly increased the leaf area, chlorophyll content, photosynthetic efficiency and light energy utilization rate of greenhouse tomato, cucumber and pepper; 7) The organic coupling cultivation technology of ΔMP-03 suspension significantly inhibited the incidence of soil-borne diseases of greenhouse tomatoes, cucumbers and peppers, significantly improved the prevention and control effect, and reduced the occurrence and harm of soil-borne diseases of tomatoes, cucumbers and peppers.

[0088] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A method for organically coupling cultivation of vegetable facilities, characterized in that: The method comprises: digging a square ditch one month before transplanting the facility vegetable seedlings, adding the following components in sequence: corn stalks, Penicillium cyclopium 455-1 bacterial suspension, vegetable virus vaccine, peanut cake, wheat bran, farmyard manure, bacterial fertilizer, compound fertilizer, water, then covering with soil, sealing with mulch film, and after one month, drilling holes on the mulch film surface to transplant the vegetable seedlings; Among them, the preservation number of Penicillium arcus 455-1 is CGMCC No.41541.

2. The method according to claim 1, characterized in that In terms of weight percentage, the addition ratio of each component is as follows: 50% corn stalks, 8.2% peanut cakes, 8.2% wheat bran, 9.7% farmyard manure, 6.8% bacterial fertilizer, 5.2% compound fertilizer, 6.8% water, 5% Penicillium arcus 455-1 suspension with a concentration of 200 million spores / mL, and 0.1% vegetable virus vaccine; The main components of the farmyard manure are organic matter, nitrogen, phosphorus and potassium; The bacterial fertilizer is Jinzhengda Qintu No. 1; The main components of the compound fertilizer are nitrogen, phosphorus and potassium.

3. The method according to claim 2, characterized in that The vegetable virus vaccine is a attenuated strain vaccine or a double-linked small RNA vaccine, purchased from Nanjing Ruimate Biotechnology Co., Ltd.

4. The method according to any one of claims 1 to 3, characterized in that: The vegetables include tomatoes, peppers and cucumbers.

5. Use of the method according to any one of claims 1 to 4 in preventing and controlling soil-borne diseases of tomatoes, peppers and cucumbers.

6. The use according to claim 5, characterized in that: The diseases include tomato late blight caused by Phytophthora infestans, cucumber blight caused by Phytophthora drechsleri, and pepper blight caused by Phytophthora capsici.

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