A fulvic acid hydrogen peptide biological stimulant fertilizer and a preparation method thereof

Abstract

The application discloses a kind of fulvic acid hydrogen peptide biological stimulant hormone fertilizer and preparation method thereof, belong to fertilizer technical field.Fertilizer is by 15~30 parts of fulvic acid, 5~12 parts of peony seed meal small molecule peptide, 20~35 parts of potassium dihydrogen phosphate and other components compound, water is made up to 100 parts, and auxiliary agent is emulsifier and chelating agent compound.Preparation first by dissolving fulvic acid, potassium phosphate in water and obtaining mixed solution, then adding small molecule peptide and auxiliary agent and stirring until the system is stable;Small molecule peptide is prepared by peony seed meal defatted powder crushing and enzymolysis, solves the problem of traditional fertilizer nutrient antagonism, unstable system, and the utilization of peony seed meal also improves resource utilization.Tomato, cucumber seedling test shows that it can significantly improve the growth index of crop root system and aboveground part, and the root-promoting and seedling-strengthening effect is better than that of similar fertilizer on the market, and the preparation process is simple, easy to industrialization, and has good agricultural application prospect.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology, and in particular to a humic acid hydrogen peptide biostimulant fertilizer and its preparation method. Background Technology

[0002] In agricultural planting, root development and seedling vigor directly determine crop growth, stress resistance, and ultimately, yield and quality. Therefore, promoting root and seedling growth is a crucial aspect of crop cultivation management, and corresponding functional fertilizers have become one of the core agricultural inputs in agricultural production. Currently, there are many types of root and seedling growth fertilizers on the market, mainly including single or compound fertilizers such as humic acid, amino acids, and phosphorus and potassium elements. However, existing products generally have many shortcomings: some fertilizers only focus on supplementing nutrients and lack the synergistic effect of bioactive substances, resulting in slow root promotion and limited improvement in seedling stress resistance; some fertilizers containing active peptides are mostly peptides prepared from common animal and plant proteins, which have low activity, poor compatibility with mineral elements, and are prone to nutrient antagonism; and some compound fertilizers have unreasonable component combinations, with poor integration of fulvic acid with other nutrients, easily leading to stratification and precipitation, resulting in low nutrient utilization after fertilizer application and failing to achieve the effect of synergistic root and seedling growth.

[0003] Meanwhile, traditional fulvic acid fertilizers often suffer from insufficient content of small-molecule active substances and reduced effectiveness after combining with elements such as phosphorus and potassium. Peptide fertilizers, on the other hand, rely heavily on grain protein raw materials, resulting in high costs and limited raw material sources. Peony seed meal, a byproduct of peony seed oil processing, is rich in high-quality plant protein, yet it is mostly discarded or used as cheap animal feed, leading to extremely low resource utilization and waste of raw materials. Furthermore, existing root-promoting and seedling-strengthening fertilizers often involve complex preparation processes, some requiring high-temperature and high-pressure treatments that can easily destroy active ingredients. Inappropriate selection of adjuvants can also lead to poor fertilizer system stability, resulting in performance degradation during storage and application, making it difficult to meet the demands of efficient, stable, and environmentally friendly fertilization in agricultural production.

[0004] Therefore, developing a compound fertilizer with fulvic acid and highly active small molecule peptides as its core, combined with a reasonable phosphorus and potassium nutrient system, using environmentally friendly and highly utilized raw materials, exhibiting synergistic effects between components and good system stability, and capable of rapidly promoting root growth and seedling development, while also having a simple preparation process and being easy to industrialize, has become an urgent technical problem to be solved in the field of agricultural fertilizers. Summary of the Invention

[0005] The purpose of this invention is to overcome the technical defects of existing root-promoting and seedling-strengthening fertilizers, such as poor nutrient synergy, low utilization rate of active ingredients, and waste of raw materials. This invention provides a fulvic acid hydrogen peptide biostimulant fertilizer and its preparation method. By using fulvic acid and peony seed meal small molecule peptides as core active ingredients, combined with phosphorus and potassium nutrients and special adjuvants, the components synergistically enhance each other, which can effectively promote crop root development and cultivate strong seedlings, and can effectively meet the actual needs of agricultural production.

[0006] This application provides a fulvic acid hydrogen peptide biostimulant fertilizer, which comprises the following components by weight: 15-30 parts fulvic acid, 5-12 parts small molecule peptide, 20-35 parts potassium dihydrogen phosphate, 1-5 parts monoammonium phosphate, 0.1-0.8 parts potassium sulfate, 1-4 parts adjuvant, and water to make up to 100 parts.

[0007] Preferably, the small molecule peptide is a small molecule peptide derived from peony seed meal.

[0008] Preferably, the fulvic acid is either mineral-derived fulvic acid or biochemical fulvic acid.

[0009] Preferably, the additives include emulsifiers and chelating agents.

[0010] Preferably, the emulsifier is either sodium dodecylbenzenesulfonate or Tween 80.

[0011] Preferably, the chelating agent is any one of tartaric acid, citric acid, and sodium citrate.

[0012] This application also provides a fulvic acid hydrogen peptide biostimulant fertilizer and a method for preparing the same, the preparation method comprising the following steps:

[0013] (1) Add fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate and potassium sulfate to water according to the ratio, and stir until completely dissolved to obtain a mixed solution;

[0014] (2) Add small molecule peptides to the mixture and stir until homogeneous;

[0015] (3) Add the adjuvant and continue stirring until the system is uniform and stable to obtain the root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer.

[0016] Preferably, the method for preparing the small molecule peptides is as follows: after defatting and pulverizing peony seed meal, the pH is adjusted, a compound protease is added for enzymatic hydrolysis, the enzyme is inactivated, and the residue is removed by centrifugation to obtain peony seed meal small molecule peptide solution.

[0017] Preferably, the enzymatic hydrolysis is performed at 45–55°C for 2–4 hours.

[0018] Preferably, the enzyme inactivation parameters are 85-95℃ for 10-15 minutes.

[0019] The beneficial effects of the embodiments in this application are as follows:

[0020] (1) As the core active ingredient, fulvic acid has strong biological activity. It can stimulate the division and elongation of crop root cells, induce the root system to produce auxin-like substances, promote the thickening and elongation of the main root and the germination of lateral roots, and increase the root absorption area. It can also improve the soil aggregate structure, improve the soil's water and fertilizer retention capacity, and chelate mineral elements in the soil to reduce nutrient fixation. It can enhance the permeability of crop cell membranes, improve the seedling's resistance to stress, alleviate the inhibition of seedling growth by environmental stress, and can also combine with other nutrients to improve the compatibility of the fertilizer system.

[0021] (2) Peony seed meal small molecule peptides use peony seed oil processing by-products as raw materials to achieve high-value utilization of resources and reduce raw material costs. It contains a variety of amino acids and active peptides, which are easily absorbed directly by crop roots, quickly replenishing the nitrogen source and bioactive substances required for seedling growth; it can activate enzymatic reactions in the plant, promote protein synthesis and nutrient metabolism, help increase the height and stem diameter of the above-ground parts of seedlings, and enhance seedling growth; at the same time, it works synergistically with fulvic acid to enhance root vitality, reduce nutrient antagonism, and further strengthen the root-promoting effect.

[0022] (3) Fulvic acid and peony seed meal small molecule peptides have a synergistic effect. Compared with the control group 1 without the addition of peony seed meal small molecule peptides, the main root length and seedling height of crops can be significantly increased, and the roots and aboveground parts can grow vigorously at the same time. The synergistic effect of roots and seedlings is far better than the nutritional system of fulvic acid alone. The experiment verified that the main root length of tomato and cucumber seedlings was significantly increased compared with the control group 1, and the growth effect of plant height and stem diameter was also better. Attached Figure Description

[0023] Figure 1 This is a flowchart of the preparation method of Example 1 of this application. Detailed Implementation

[0024] The technical solutions of this application will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0025] In the following examples, peony seed meal was purchased from Heze Guyu Peony Biotechnology Co., Ltd.; compound protease (100,000 U / g) was purchased from Henan Wansheng Industrial Co., Ltd.; and other reagents and equipment not specified were conventional and commercially available.

[0026] Preparation Example 1

[0027] Preparation of small molecule peptides from peony seed meal

[0028] (1) Raw material processing: Peony seed meal was degreased with petroleum ether at a material-to-liquid ratio of 1:5, degreased at room temperature for 2 hours, pulverized to 80 mesh, and sieved.

[0029] (2) pH adjustment: Add deionized water to the crushed peony seed meal at a material-to-liquid ratio of 1:40, and adjust the pH of the system to 7.0 with 1 mol / L NaOH.

[0030] (3) Enzymatic hydrolysis: Add compound protease at a dosage of 5000 U / g, and hydrolyze in a constant temperature water bath at 50℃ for 4 hours with a stirring rate of 150 r / min.

[0031] (4) Enzyme inactivation: Heat to 90℃ and maintain the temperature for 12 minutes to complete enzyme inactivation;

[0032] (5) Separation: Centrifuge at 4000r / min for 15min, take the supernatant to obtain peony seed meal small molecule peptide solution.

[0033] Preparation Example 2

[0034] Preparation of small molecule peptides from peony seed meal

[0035] (1) Raw material processing: Peony seed meal was degreased with petroleum ether at a material-to-liquid ratio of 1:5, degreased at room temperature for 2 hours, pulverized to 80 mesh, and sieved.

[0036] (2) pH adjustment: Add deionized water to the crushed peony seed meal at a material-to-liquid ratio of 1:40, and adjust the pH of the system to 7.0 with 1 mol / L NaOH.

[0037] (3) Enzymatic hydrolysis: Add compound protease at a dosage of 5000 U / g, and hydrolyze in a constant temperature water bath at 45℃ for 4 hours with a stirring rate of 150 r / min.

[0038] (4) Enzyme inactivation: Heat to 85℃ and maintain the temperature for 10 minutes to complete enzyme inactivation;

[0039] (5) Separation: Centrifuge at 4000r / min for 15min, take the supernatant to obtain peony seed meal small molecule peptide solution.

[0040] Preparation Example 3

[0041] Preparation of small molecule peptides from peony seed meal

[0042] (1) Raw material processing: Peony seed meal was degreased with petroleum ether at a material-to-liquid ratio of 1:5, degreased at room temperature for 2 hours, pulverized to 80 mesh, and sieved.

[0043] (2) pH adjustment: Add deionized water to the crushed peony seed meal at a material-to-liquid ratio of 1:40, and adjust the pH of the system to 7.0 with 1 mol / L NaOH.

[0044] (3) Enzymatic hydrolysis: Add compound protease at a dosage of 5000 U / g, and hydrolyze in a constant temperature water bath at 55℃ for 2 hours with a stirring rate of 150 r / min.

[0045] (4) Enzyme inactivation: Heat to 95℃ and maintain the temperature for 15 minutes to complete enzyme inactivation;

[0046] (5) Separation: Centrifuge at 4000r / min for 15min, take the supernatant to obtain peony seed meal small molecule peptide solution.

[0047] Example 1

[0048] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0049] (1) Formula components: by weight, 20 parts of mineral fulvic acid, 8 parts of peony seed meal small molecule peptides prepared in Example 1, 28 parts of potassium dihydrogen phosphate, 3 parts of monoammonium phosphate, 0.5 parts of potassium sulfate, 2 parts of auxiliary agents (1 part of sodium dodecylbenzenesulfonate and 1 part of tartaric acid), and 38.5 parts of deionized water.

[0050] (2) Preparation method: Its flowchart is as follows Figure 1 As shown:

[0051] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0052] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0053] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour sodium dodecylbenzenesulfonate into the mixing tank, stir for 5 min to make it evenly dispersed in the system, then add tartaric acid in batches and small amounts, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Use a viscometer to measure the viscosity of 18 mPa·s humic acid hydrogen peptide biostimulant fertilizer.

[0054] Example 2

[0055] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0056] (1) Formula components: by weight, 15 parts of mineral fulvic acid, 5 parts of peony seed meal small molecule peptides prepared in Example 2, 20 parts of potassium dihydrogen phosphate, 1 part of monoammonium phosphate, 0.1 parts of potassium sulfate, 1 part of auxiliary agent (0.5 parts of Tween 80, 0.5 parts of citric acid), and 57.9 parts of deionized water.

[0057] (2) Preparation method:

[0058] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0059] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0060] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour Tween 80 into the mixing tank, stir for 5 min to make it evenly dispersed in the system, then add citric acid in batches of small amounts, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Measure the viscosity with a viscometer to 19 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0061] Example 3

[0062] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0063] (1) Formula components: by weight, 30 parts of biochemical fulvic acid, 12 parts of peony seed meal small molecule peptides prepared in Example 3, 35 parts of potassium dihydrogen phosphate, 5 parts of monoammonium phosphate, 0.8 parts of potassium sulfate, 4 parts of adjuvants (2 parts of Tween 80 and 2 parts of sodium citrate), and 13.2 parts of deionized water.

[0064] (2) Preparation method:

[0065] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0066] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0067] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour Tween 80 into the mixing tank, stir for 5 min to evenly disperse it in the system, then add sodium citrate in small batches, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Measure the viscosity with a viscometer to 18 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0068] Example 4

[0069] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0070] (1) Formula components: by weight, 20 parts of biochemical fulvic acid, 8 parts of peony seed meal small molecule peptides prepared in Example 1, 28 parts of potassium dihydrogen phosphate, 3 parts of monoammonium phosphate, 0.5 parts of potassium sulfate, 2 parts of auxiliary agents (1 part of sodium dodecylbenzenesulfonate and 1 part of citric acid), and 38.5 parts of deionized water.

[0071] (2) Preparation method:

[0072] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0073] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0074] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour sodium dodecylbenzenesulfonate into the mixing tank, stir for 5 min to disperse it evenly in the system, then add citric acid in small batches, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Measure the viscosity with a viscometer to 17 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0075] Example 5

[0076] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0077] (1) Formula components: by weight, 20 parts of biochemical fulvic acid, 8 parts of peony seed meal small molecule peptides prepared in Example 3, 28 parts of potassium dihydrogen phosphate, 3 parts of monoammonium phosphate, 0.5 parts of potassium sulfate, 2 parts of auxiliary agents (1 part of sodium dodecylbenzenesulfonate and 1 part of sodium citrate), and 38.5 parts of deionized water.

[0078] (2) Preparation method:

[0079] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0080] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0081] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour sodium dodecylbenzene sulfonate into the mixing tank, stir for 5 min to disperse it evenly in the system, then add sodium citrate in small batches, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Measure the viscosity with a viscometer to 18 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0082] Example 6

[0083] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0084] (1) Formula components: by weight, 20 parts of mineral fulvic acid, 8 parts of peony seed meal small molecule peptides prepared in Example 2, 28 parts of potassium dihydrogen phosphate, 3 parts of monoammonium phosphate, 0.5 parts of potassium sulfate, 2 parts of auxiliary agents (1 part of Tween 80 and 1 part of tartaric acid), and 38.5 parts of deionized water.

[0085] (2) Preparation method:

[0086] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0087] 2) Addition of small molecule peptides from peony seed meal: Keep the stirring state of the mixing vessel unchanged and maintain the stirring rate at 250 r / min. Slowly and evenly add the measured peony seed meal small molecule peptide solution into the mixing vessel, with the addition rate controlled at 5-10 mL / min. After all the peptide solution has been added, continue to maintain the stirring rate at 250 r / min and continue stirring for 20 min. During the stirring process, observe the state of the system regularly to ensure that there is no local aggregation or layering. After stirring, the system is a uniform light brown or light yellow liquid.

[0088] 3) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour Tween 80 into the mixing tank, stir for 5 min to evenly disperse it in the system, then add tartaric acid in small batches, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Measure the viscosity with a viscometer to 16 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0089] Comparative Example 1

[0090] Root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer

[0091] (1) Formula components: by weight, 20 parts mineral humic acid, 28 parts potassium dihydrogen phosphate, 3 parts monoammonium phosphate, 0.5 parts potassium sulfate, 2 parts auxiliary agents (1 part Tween 80, 1 part tartaric acid), and 38.5 parts deionized water.

[0092] (2) Preparation method:

[0093] 1) Preparation of basic mixture: Add deionized water to a clean and dry stirred tank, turn on the stirring equipment, adjust the stirring speed to 250 r / min, and stir in a clockwise direction (to ensure uniform stirring without dead corners); after the stirring is stable, slowly add the mineral fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate, and potassium sulfate in batches to the weighed solid raw materials. After each raw material is added, continue stirring for 5 minutes until the raw material is initially dissolved before adding the next one. This avoids the simultaneous addition of multiple raw materials, which may lead to excessively high local concentrations, clumping, and difficulty in dissolving. After all raw materials have been added, maintain the stirring speed of 250 r / min and continue stirring for 30 minutes. After stirring, turn off the stirring equipment and let it stand for 5 minutes. The basic mixture should be completely transparent or translucent and free of visible solid particles.

[0094] 2) Additive addition: Adjust the stirring speed of the mixing tank to 200 r / min, slowly pour Tween 80 into the mixing tank, stir for 5 min to make it evenly dispersed in the system, then add tartaric acid in batches of small amounts, stirring for 3-5 min after each addition, maintaining a stirring speed of 200 r / min, and continue stirring for 25 min. During the stirring process, observe the state of the system every 8 min to check for stratification, precipitation, and bubbles. Use a viscometer to measure the viscosity to 14 mPa·s. This is fulvic acid hydrogen peptide biostimulant fertilizer.

[0095] Test case

[0096] Fertilizer comparison test

[0097] (1) Experimental materials: test fertilizers: root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer prepared in Examples 1-6 of this invention; root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer of Comparative Example 1; Comparative Example 2: commercially available humic acid phosphorus and potassium fertilizer; Comparative Example 3: commercially available amino acid peptide fertilizer; blank control group: water.

[0098] (2) Crops: Tomato: Variety: Fen Guan No. 2; Cucumber: Variety: Jin Yan No. 4; Select plump and uniformly sized seeds, and after soaking and germination, select seeds with uniform white sprouts for sowing.

[0099] (3) Substrate: peat moss: vermiculite: perlite = 3:1:1. After high-temperature sterilization, the substrate is filled into seedling pots, with 200g of substrate per pot.

[0100] (4) Experimental methods: The experiment was conducted in a greenhouse, with 10 treatment groups: Examples 1-6, Comparative Example 1 (root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer), Comparative Example 2, Comparative Example 3, and a blank control group. Each group was replicated three times, with 30 seedlings per replicate, arranged in a randomized block design. Greenhouse environmental parameters: temperature 25±2℃, light duration 12h / d, light intensity 30000lx, relative humidity 60%-70%.

[0101] (5) Test procedure:

[0102] 1) Sowing and seedling raising: Sow the germinated tomato and cucumber seeds separately in seedling pots, one seed per pot, cover with 1cm of soil, water thoroughly, and start fertilizer spraying when the seedlings grow to two leaves and one heart.

[0103] 2) Fertilization method and concentration: All tested fertilizers were diluted 500 times and applied by a combination of foliar spraying and root irrigation. The treatment was carried out once every 7 days for 3 consecutive treatments. The amount of fertilizer sprayed / irrigated per plant each time was 20 mL. The blank control group was treated with an equal amount of water. All other cultivation and management measures were kept the same.

[0104] 3) Measurement indicators and time: Seven days after the last treatment, 10 seedlings of uniform growth were randomly selected from each group (replicas) and the following indicators were measured:

[0105] a. Root system indicators: taproot length (cm), number of lateral roots (strips / plant), fresh root weight (g / plant), dry root weight (g / plant, dried at 80℃ to constant weight).

[0106] b. Above-ground indicators of seedlings: plant height (cm), stem diameter (mm, 1cm below cotyledon), number of true leaves (leaf / plant), above-ground fresh weight (g / plant), above-ground dry weight (g / plant, dried at 80℃ to constant weight).

[0107] c. Comprehensive index: root-to-shoot ratio (root dry weight / aboveground dry weight).

[0108] (6) Experimental results: The growth index data of tomato seedlings are shown in Table 1; the growth index data of cucumber seedlings are shown in Table 2.

[0109] Table 1. Growth Indicators of Tomato Seedlings

[0110] Main root length (cm) Number of lateral roots (per plant) Root dry weight (g / plant) Plant height (cm) Stem diameter (mm) Example 1 Group 18.5±0.9 32.3±1.2 0.58±0.09 22.6±1.3 4.5±0.2 Example 2 group 17.2±1.6 30.1±1.0 0.52±0.12 21.7±0.5 4.2±0.5 Example 3 Group 19.1±1.2 33.5±1.3 0.61±0.06 23.5±1.0 4.7±0.9 Example 4 group 18.2±0.6 31.8±1.5 0.63±0.04 22.3±0.8 4.4±0.6 Example 5 group 18.5±1.5 31.5±0.8 0.55±0.10 22.0±0.7 4.3±0.8 Example 6 group 18.8±1.2 32.8±1.4 0.59±0.08 23.0±1.6 4.6±1.1 Comparative Example 1 15.3±0.9 30.5±1.3 0.65±0.15 20.8±1.4 4.2±0.6 Comparative Example 2 14.3±0.8 21.5±1.9 0.38±0.11 20.5±1.2 3.5±0.7 Comparative Example 3 Groups 15.1±1.4 23.2±0.7 0.41±0.05 20.9±1.5 3.7±1.3 Blank control group 10.2±1.7 12.8±1.6 0.22±0.13 18.6±0.9 2.8±0.9

[0111] Table 2. Growth Indicators of Cucumber Seedlings

[0112] Main root length (cm) Number of lateral roots (per plant) Root dry weight (g / plant) Plant height (cm) Stem diameter (mm) Example 1 Group 16.8±0.5 28.5±1.1 0.51±0.02 20.3±0.7 4.2±0.5 Example 2 group 15.7±0.9 26.3±0.9 0.46±0.06 19.2±0.9 3.9±0.9 Example 3 Group 17.5±1.3 29.8±1.2 0.55±0.10 21.2±0.5 4.5±0.7 Example 4 group 16.5±1.1 28.1±1.5 0.49±0.07 20.0±1.4 4.1±1.2 Example 5 group 16.3±1.6 27.8±1.7 0.48±0.05 21.8±1.6 4.0±0.8 Example 6 group 17.2±1.5 29.3±1.1 0.53±0.08 20.8±1.9 4.4±0.9 Comparative Example 1 14.9±0.12 27.5±1.9 0.52±0.11 19.5±1.2 3.6±0.5 Comparative Example 2 12.5±0.7 19.2±0.8 0.33±0.09 18.5±1.3 3.2±1.3 Comparative Example 3 Groups 13.2±0.8 20.5±0.9 0.36±0.03 19.6±1.5 3.4±0.4 Blank control group 8.8±1.2 11.5±1.2 0.19±0.06 16.3±1.7 2.5±0.6

[0113] (7) Experimental Analysis:

[0114] Tables 1 and 2 show that after applying the humic acid hydrogen peptide fertilizers of Examples 1-6, the taproot length, lateral root number, and root dry weight of tomato and cucumber seedlings were significantly higher than those of Comparative Examples 2 and 3, and the blank control group. Specifically, the average taproot length of tomato seedlings in the Example groups was approximately 18.4 cm, an increase of 28.7% compared to Comparative Example 2 and 80.4% compared to the blank control group; the average taproot length of cucumber seedlings in the Example groups was approximately 16.7 cm, an increase of 33.6% compared to Comparative Example 2 and 90.9% compared to the blank control group; regarding the number of lateral roots, the average number of lateral roots in tomato seedlings in the Example groups was significantly higher than that in comparative examples 2 and 3, and the blank control group. The average number of lateral roots per plant was over 30 for tomatoes and over 27 for cucumbers, while the commercially available fertilizer group had less than 24 lateral roots per plant for tomatoes and less than 21 lateral roots per plant for cucumbers, and the blank group had only about 10 lateral roots per plant. The average dry weight of the root system in the example groups was about 0.58 g / plant for tomatoes and about 0.50 g / plant for cucumbers, which was more than twice that of the blank group and about 40%-60% higher than that of the commercially available fertilizer group. The data on main root length and plant height in Example 1-6 groups were significantly higher than those in Comparative Example 1, indicating that the addition of peony seed meal small molecule peptides has a significant beneficial effect on main root length and plant height.

[0115] In summary, the root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer developed in this invention uses humic acid and small-molecule peptides from peony seed meal as its core active ingredients, combined with a reasonable ratio of phosphorus and potassium nutrients and special emulsifying and chelating agents. This solves the technical defects of traditional root-promoting and seedling-strengthening fertilizers, such as poor nutrient synergy, low utilization rate of active ingredients, raw material waste, and poor system stability. The fertilizer is prepared using a simple room-temperature stirring process, eliminating the need for high-temperature and high-pressure treatment, effectively preserving the activity of each component. Furthermore, the resource utilization of peony seed meal reduces raw material costs and achieves environmentally friendly reuse of agricultural by-products. Field trials on tomato and cucumber seedlings have verified that after applying this fertilizer, the root indicators such as taproot length, lateral root number, and root dry weight, as well as the above-ground growth indicators such as plant height and stem diameter, are significantly better than those of commercially available humic acid phosphorus and potassium fertilizers, amino acid peptide fertilizers, and the water control group. It can efficiently promote crop root development and cultivate robust seedlings, with outstanding synergistic effects on root and seedling growth, demonstrating good application prospects and promotional value in the agricultural planting field.

[0116] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application. All equivalent changes and improvements made within the scope of this application shall still fall within the patent coverage of this application.

Claims

1. A fulvic acid hydrogen peptide biostimulant fertilizer, characterized in that, The fertilizer comprises the following components by weight: 15-30 parts fulvic acid, 5-12 parts small molecule peptides, 20-35 parts potassium dihydrogen phosphate, 1-5 parts monoammonium phosphate, 0.1-0.8 parts potassium sulfate, 1-4 parts adjuvants, and water to make up to 100 parts.

2. The fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 1, characterized in that, The small molecule peptide is a small molecule peptide derived from peony seed meal.

3. The fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 1, characterized in that, The fulvic acid is either mineral-derived fulvic acid or biochemical fulvic acid.

4. The fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 1, characterized in that, The additives include emulsifiers and chelating agents.

5. The fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 4, characterized in that, The emulsifier is either sodium dodecylbenzenesulfonate or Tween 80.

6. The fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 4, characterized in that, The chelating agent is any one of tartaric acid, citric acid, and sodium citrate.

7. A method for preparing a fulvic acid hydrogen peptide biostimulant fertilizer, characterized in that, The preparation method includes the following steps: (1) Add fulvic acid, potassium dihydrogen phosphate, monoammonium phosphate and potassium sulfate to water according to the ratio, and stir until completely dissolved to obtain a mixed solution; (2) Add small molecule peptides to the mixture and stir until homogeneous; (3) Add the adjuvant and continue stirring until the system is uniform and stable to obtain the root-promoting and seedling-strengthening humic acid hydrogen peptide fertilizer.

8. The method for preparing a fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 7, characterized in that, The method for preparing the small molecule peptides is as follows: after defatting and pulverizing peony seed meal, the pH is adjusted, a compound protease is added for enzymatic hydrolysis, the enzyme is inactivated, and the residue is removed by centrifugation to obtain peony seed meal small molecule peptide solution.

9. The preparation method of the fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 8, characterized in that, The enzymatic hydrolysis is carried out at 45–55°C for 2–4 hours.

10. The method for preparing a fulvic acid hydrogen peptide biostimulant fertilizer as described in claim 8, characterized in that, The enzyme inactivation parameters are 85-95℃ for 10-15 minutes.

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