Method for preparing biopesticide tetramycin and application

A technology of Wuningmycin and bio-pesticides, applied in the field of application in the prevention and treatment of apple spot leaf defoliation, can solve the problems of limited use and efficacy, unsatisfactory control effect of apple rot disease, and high cost

Active Publication Date: 2010-01-27
辽宁微科生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyoxin is the main biopesticide used for apple spot deciduous disease, but polyoxin is mostly imported preparations, which increases the cost. It is urgent to research and develop new biopesticides to solve the high cost and low control rate of existing biopesticides And other issues
[0007] Wuningmycin, as a known compound, is produced by Streptomyces ahygroscopicus subsp.Wuzhouensis n.subsp ("Acta Mi

Method used

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  • Method for preparing biopesticide tetramycin and application
  • Method for preparing biopesticide tetramycin and application
  • Method for preparing biopesticide tetramycin and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment 1: the preparation of Wuningmycin fermented liquid

[0103] The present invention passes through the preferred culture medium formula of orthogonal test:

[0104] (1) Seed fermentation medium: every 100 ml contains: soybean cake powder 1.0g, starch 1.0g, glucose 0.7g, peptone 0.5g, magnesium sulfate 0.03g, ammonium sulfate 1.0g, dipotassium hydrogen phosphate 0.02g, Calcium carbonate 0.7g, sodium chloride 0.1g, vegetable oil 0.2g, pH natural;

[0105](2) Submerged fermentation medium: every 100 ml contains: glucose 2.5g, starch 1.5g, cornmeal 1.0g, soybean cake powder 2.0g, peptone (or corn steep liquor) 0.5g, ammonium sulfate 0.2g, magnesium sulfate 0.03g, 0.02g of dipotassium hydrogen phosphate, 0.7g of calcium carbonate, 0.1g of sodium chloride, 0.2g of vegetable oil, after sterilization, add 0.4% of the medium volume of n-propanol, and the pH is natural.

[0106] The present invention has optimized fermentation condition:

[0107] (1) Preparation of se...

Embodiment 2-5

[0109] Example 2-5: Application examples of Wuningmycin in the prevention and treatment of apple spot defoliation disease

[0110]

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PUM

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Abstract

The invention discloses a method for preparing biopesticide tetramycin from Streptomyces ahygroscopicus subsp.Wuzhouensis n.subsp, and application of a product prepared by the method in preventing and controlling diseases such as apple alternaria leaf spots and the like. The tetramycin prepared by the method improves the biological potency of the tetramycin (the biological potency of fermentation liquor is improved by 15.5 percent after 0.3 to 0.5 percent of normal propyl alcohol is added.), can prolong the quality guarantee period, improves the effect of preventing and controlling apple canker to over 95 percent, has good effect of preventing and controlling the diseases such as the apple alternaria leaf spots, tomato gray mold and the like, has effect of inhibiting yeast, gram-negative and gram positive bacteria, fungi (comprising fungal pathogens) and the like, has wide adaptability, and belongs to the environment-friendly biopesticide.

Description

technical field [0001] The invention relates to an improved preparation method of Wuningmycin, a biopesticide produced by Streptomyces ahygroscopicus subsp. Wuzhouensis n.subsp, and the application of the product in preventing and treating apple leaf spot disease. Background technique [0002] my country is a large agricultural country, with more than 100 million hectares of grain crops, more than 4.8 million hectares of cotton, more than 14 million hectares of oil crops, and more than 18 million hectares of vegetables and fruits. For a long time, people have used a lot of chemical pesticides to prevent and control plant diseases and insect pests. The use of chemical pesticides plays an effective role in ensuring the normal production of crops, but with the long-term use of a large number of chemical pesticides, it has caused serious damage to the ecological environment, such as soil structure damage, reduced enzyme activity in the soil, serious pollution of soil and water, e...

Claims

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Application Information

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IPC IPC(8): C12P17/18A01N43/90A01P3/00C12R1/465
Inventor 李莉陈飞王志学韩希军吴红艳桓明辉关艳丽郭玲玲
Owner 辽宁微科生物工程有限公司
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