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Anti-phytoplasma composition containing lentinan

A technology of lentinan and phytoplasma, which is applied in the directions of plant growth regulators, botanical equipment and methods, animal repellents, etc., can solve the problems of few effective control agents and poor safety, and achieves good effects and high field control efficiency. Effect

Active Publication Date: 2017-11-07
SHANDONG SHENGPENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of less effective agents and poor safety for the prevention and control of phytoplasma diseases, the present invention provides an anti-phytoplasma composition containing lentinan, which has good biological activity and high safety to crops

Method used

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  • Anti-phytoplasma composition containing lentinan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of Lentinan and Oligoaminosaccharin Preparations

[0015] 1.1 5% lentinan and amino oligosaccharin (3:8) aqueous solution

[0016] Composition by weight percentage:

[0017] Lentinan 1.36%

[0018] Oligoaminosaccharins 3.64%

[0019] Sodium dodecylbenzenesulfonate 1%

[0020] Styrylphenyl ethoxylate 4%

[0021] Ammonium sulfate 6%

[0022] Water up to 100%

[0023] Preparation:

[0024] Dissolve ammonium sulfate in water, add sodium dodecylbenzene sulfonate and styrylphenyl polyoxyethylene ether, mix evenly, add lentinan and amino oligosaccharin master drug, stir evenly, and the product is obtained.

[0025] 1.2 4% lentinan and amino oligosaccharin (4:7) water

[0026] Lentinan 1.45%

[0027] Oligoaminosaccharins 2.55%

[0028] Sodium dodecylbenzenesulfonate 2%

[0029] Styrylphenol formaldehyde resin Polyoxyethylene polyoxypropylene block polyether 4%

[0030] Ethylene glycol 6%

[0031] Xanthan Gum 0.7%

[0032] Water up to 100%

...

Embodiment 2

[0061] Example 2 Indoor bioassay

[0062] Referring to the tissue culture method of Zhao Jin et al. (Feasibility study on drug screening for jujube madness under in vitro conditions [J] Journal of Hebei Agricultural University. 2006, 2:9, 1:70-73.), in sterile conditions Next, the stems (with 4 leaves) of the tissue-cultured seedlings of Jujube jujube with typical clustering symptoms were treated with drugs, and the same seedlings were inserted into MS medium containing different doses of drugs, and the concentrations of each drug were 0.1 , 1, 2, 5, 10mg / mL, each treatment repeated 3 times, 12 bottles as a treatment, light 1400-1600lx, photoperiod 16h / 8h, temperature 25-30°C, the survey results after 30 days of cultivation, using SPSS 13.0 Calculate EC 50 with the regression equation. Among them, co-toxicity coefficient (CTC) ≥ 120 means synergistic effect; 80 < CTC < 120 means additive effect; CTC ≤ 80 means antagonistic effect. The test results are shown in Table 1.

[...

Embodiment 3

[0066] Embodiment 3 field efficacy test

[0067] 3.1 Drugs to be tested

[0068] 4% lentinan and amino oligosaccharin (4:7) aqueous solution (AS): Example 1.2;

[0069] 10% tetracycline hydrochloride aqueous solution (AS);

[0070] 20% morpholinidine hydrochloride wettable powder (WP).

[0071] 3.2 Test crops and test locations

[0072] Jinsixiaozao No. 4, Zhuji Township, Leling County, Dezhou City, Shandong Province

[0073] 3.3 Dosage of treatment agents

[0074] Table 2 The amount of reagent used for testing

[0075]

[0076] 3.4 Community design

[0077] A total of 30 plots, 20 jujube trees per plot, repeated 5 times, arranged in random blocks.

[0078] 3.5 Application method

[0079] Use a knapsack-type electric sprayer to evenly spray the front and back sides of the jujube tree that has just sprouted, and the water consumption is 450kg / hm 2 , and the blank control was sprayed with the same amount of water.

[0080] The frequency of spraying was 3 times, and th...

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Abstract

The invention provides an anti-phytoplasma composition containing lentinan, wherein the active ingredient of the composition is lentinan and amino-oligosaccharin; the weight ratio of the lentinan and amino-oligosaccharin is (1 to 20) to (20 to 1); the total content of the two is 0.1 to 20wt%, and the rest is accessories. The using method of the anti-mycoplasma composition is blending with water for spraying or injecting into a plant transport system. The anti-phytoplasma composition containing lentinan has prevention and treatment effects on phytoplasma diseases, good effects, high field prevention effects and effective components safe to plants, people and livestock.

Description

technical field [0001] The invention relates to a composition for resisting phytoplasma, in particular to a composition composed of lentinan and amino oligosaccharin used in phytoplasma plant diseases, and belongs to the field of phytochemical protection. Background technique [0002] Phytoplasma, formerly known as mycoplasma-like organism (MLO), is a single-celled prokaryotic organism without a cell wall and surrounded by a biofilm. Phytoplasma cannot be cultivated artificially, and colonizes in the intestinal tract, lymph, salivary gland and other tissues of plant sieve tube cells and piercing-sucking mediator insects. Many plant diseases that were once thought to be caused by viruses have been confirmed to be caused by phytoplasma. Phytoplasmas are mainly transmitted by grafting or insects that feed on the phloem of plants, including leafhoppers, planthoppers, and aphids, which often cause plant yellowing, leaf changes, and arbuscular branches, causing huge losses to com...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00
CPCA01N43/16
Inventor 魏来忠殷碧秋张秀焕卞曙光
Owner SHANDONG SHENGPENG TECH
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