Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis

A technology of Bacillus aureus and thuringiensis spore, applied in the application, biocide, animal repellent, etc., can solve the problems of long insecticidal cycle, high cost, and easy to produce resistance, and achieve shortened insecticidal cycle and field control. the effect of improving

Pending Publication Date: 2017-02-15
JILIN ACAD OF AGRI SCI
View PDF13 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of biological pesticide that utilizes Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer, aiming to solve the problem that current Beauveria bassiana or Bacillus thuringiensis used alone to prevent and control corn borer has long killing cycle and easy to produce resistance , the problem of high cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] Such as figure 1 As shown, the preparation method of the biological pesticide utilizing Beauveria bassiana and Bacillus thuringiensis to prevent and control corn borer in the embodiment of the present invention comprises the following steps:

[0020] S101: Measure 0.1g of Beauveria bassiana (D1-5 bacterial strain) high sporopollen (spore density is 1×10 11 / g), 10g Bacillus thuringiensis (JN001 bacterial strain) high sporopollen (spore density is 1×10 12 / g), 6% auxiliary agent 1 (volume ratio)#, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% urea (mass volume Compare);

[0021] S102: After fully grinding and mixing, add sterile water to make up 1L, and keep it sealed.

Embodiment 1

[0024] Indoor toxicity test: the prepared water suspension was diluted 1000 times, and the final concentration of Beauveria bassiana spores was 1×10 4 spores / ml, the final concentration of Bacillus thuringiensis was 1×10 7 spore / ml, using the dipping method to carry out indoor virulence determination on the 2nd instar larvae of the Asian corn borer, and respectively taking the 1000-fold dilution of the suspending agent additive without Beauveria bassiana and Bacillus thuringiensis as the blank control, on the basis of the same production cost, Take Beauveria bassiana 1 x 10 9 Spores / ml was the control. The results of toxicity test showed that the LT90 of Beauveria bassiana and Bacillus thuringiensis aqueous suspension was 7.05 days, and the LT90 of Beauveria bassiana alone was 8.98 days, and the insecticidal time was shortened by 21.49%.

Embodiment 2

[0026] Field application: Dilute the prepared water-suspension agent 1000 times, and use a high-stem sprayer to carry out field spraying and control at the bell mouth stage of corn (mid-June), 20L per mu. In the second ten days of September, the insect population decline rate was investigated by dissection. Taking the untreated plot as a control, the combined control effect of Beauveria bassiana and Bacillus thuringiensis reached 85.06%, and the control effect of Beauveria bassiana alone was 66.67%, and the control effect increased by 21.62%.

[0027] The application effects of the present invention will be described in detail below in conjunction with comparison.

[0028] In application with separate 1 x 10 9 At the same cost as the concentration of Beauveria bassiana water suspension concentrate, apply 1×10 4 spores / ml Beauveria bassiana and 1×10 7 spores / ml of Bacillus thuringiensis, the combined prevention and control of corn borer can shorten the indoor insecticidal cyc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis. 1L of the biopesticide comprises 0.1g of beauveria bassiana, 10g of bacillus thuringiensis, 6% of an auxiliary agent 1# on the basis of a volume ratio, 2% of an auxiliary agent 2# on the basis of a volume ratio, 0.05% of xanthan gum on the basis of a volume ratio, 0.05% of formaldehyde on the basis of a volume ratio, 5% of urea on the basis of a mass-volume ratio and the balance water. Through combination of beauveria bassiana and bacillus thuringiensis, a water suspending agent is prepared. Through high rod sprayer spraying, Asiatic corn borers in the field can be prevented and controlled and an insecticidal period is shortened by 20%. Compared with a single beauveria bassiana water suspending agent with a beauveria bassiana concentration of 1*10<9> and with the same cost, the biopesticide utilizes combination of 1*10<4> spore / ml of beauveria bassiana and 1*10<7> spore / ml of bacillus thuringiensis to prevent and control corn borers, shortens an indoor insecticidal period by 20% or more and improves field prevention effects by 20% or more.

Description

technical field [0001] The invention belongs to the technical field of corn borer prevention and control, and in particular relates to a biological pesticide for preventing and controlling corn borer by using Beauveria bassiana and Bacillus thuringiensis. Background technique [0002] Corn is the second largest grain crop in my country, with an annual output of 300 billion catties. It can be used for food, and can also be used as feed and industrial raw materials to produce industrial alcohol and shochu. The corn borer is the main pest in corn production. It not only eats the leaves of corn, bores into the main stem or ear of corn, reduces photosynthesis and affects nutrient transportation; The yield loss of corn is 7%-15%, more than 50% yield reduction in severe years, or even no yield, causing huge economic losses to my country's grain production. Chemical pesticides have always played a major role in the prevention and control of corn borer, ensuring food production and s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/04A01N63/00A01P7/04
CPCA01N63/00A01N63/30A01N2300/00
Inventor 张正坤李启云路杨赵宇汪洋洲徐文静张佳诗杜茜隋丽
Owner JILIN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com