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Multifunctional bacillus siamensis and application thereof

A technology of bacillus and bacteria, applied in the direction of application, bacteria, fungicides, etc.

Active Publication Date: 2021-07-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some scholars screened the biocontrol bacteria with antagonistic effect on the pathogenic bacteria of peony root rot in the early stage (Wang Xueshan et al., Screening and identification of antagonistic bacteria of peony root rot. Shandong Agricultural Science, 2012, Vol. 44, No. 7; Wu Yuzhu et al., Research on 6 kinds of biocontrol fungi and bacteria to control tree peony root rot. Shandong Forestry Science and Technology, No. 6, 2004), but the research objects are all ornamental tree peonies. There are few reports on the screening of pathogenic bacteria and biocontrol bacteria

Method used

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  • Multifunctional bacillus siamensis and application thereof
  • Multifunctional bacillus siamensis and application thereof
  • Multifunctional bacillus siamensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation and identification of embodiment 1 Bacillus siamese LS275

[0020] 1. Source of soil samples

[0021] The soil was taken from the rhizosphere soil and non-rhizosphere soil of oil peony plants with root rot in Changzhi area, Shanxi Province. Uproot the plant and shake it off gently. The large piece of soil that falls is the non-rhizosphere soil, and the soil that still sticks to the root is the rhizosphere soil. The samples of every 5 plants are mixed into one soil sample, and the soil is collected into sterile Bags, labeled and numbered, brought back to the laboratory and stored in a 4°C refrigerator.

[0022] 2. Isolation of Bacillus strains

[0023] Take 10g of soil samples in 90mL of distilled water, place in a constant temperature shaking incubator for 30 minutes, and bathe in a water bath at 85°C for 30 minutes to kill most of the non-spore bacteria, let it stand, and dilute the supernatant to 10 -3 , 10 -4 , 10 -5concentration. Take 100 μL of the d...

Embodiment 2

[0043] Example 2 Determination of the growth-promoting function of Bacillus siamese LS275

[0044] Microorganisms with plant growth-promoting effects generally produce certain growth-promoting factors and secrete them into the environment, thereby promoting plant growth. Under laboratory conditions, the growth-promoting effect on plants can be judged by determining whether microorganisms produce indole-3-acetic acid (IAA), siderophore and ACC deaminase.

[0045] 1. Identification of iron carrier capacity

[0046] The activated antagonistic bacteria were inoculated in CAS medium and cultured at 28°C for 48 hours. If there was an orange-yellow transparent circle around the biocontrol strain, it indicated that it had the ability to produce siderophore, otherwise it did not (Zhan Shoufa et al., 2017).

[0047] 2. Functional identification of IAA (indole acetic acid) production

[0048]Add L-tryptophan to the liquid LB and Gao’s No. 1 medium to make the content reach 2.5mmol / L, i...

Embodiment 3

[0067] Growth and Colonization Ability of Embodiment 3 Bacillus siamese LS275

[0068] 1. Determination of growth characteristics: take Bacillus siamese LS275 seed solution and inoculate it in 20mL liquid LB medium with 1% inoculum, repeat 3 times, cultivate it in a shaker at 37°C and 120r / min, respectively at 2 and 4 , 6, 8, 10, 12, 18, 24, 30, 36, 42, 48, 54, 60, 66, and 72h to take the bacterial liquid, and use a microplate reader to measure the absorbance value (OD 590 ), with the other 2 strains of Bacillus siamese LS28, LS211 and 1 strain of Bacillus subtilis LS18 obtained through simultaneous screening as controls, the growth characteristics were measured. For the growth curves of the 4 strains of antagonistic bacteria, see figure 2 . It can be seen from the figure that the four strains of biocontrol bacteria all entered the logarithmic phase at 6-8 hours, entered the stable phase at 24 hours, and entered the decay period at 54 hours. The OD value of LS18 was the low...

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Abstract

The invention discloses a bacillus siamensis (bacillus siamensis), and the preservation number of the bacillus siamensis is CGMCC No.19505. The bacillus siamensis has a very high inhibition effect on root rot caused by fusarium solani, the bacterial liquid of the bacillus siamensis has a relatively good prevention and treatment effect on the root rot of oil peonies, the growth of oil peony plants can be promoted, so that the investment of chemical fertilizers and pesticides can be reduced, the environmental pollution can be alleviated, and the sustainable development of agriculture can be realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the field of plant disease biological control microorganisms, in particular to a strain of multifunctional Bacillus siamese and its application. Background technique [0002] Peony for oil (Paeonia suffruticosa Andr.) is a new type of woody oil crop, which has strong seed production ability and high seed oil yield. Peony seed oil is rich in unsaturated fatty acids, of which α-linolenic acid is higher than 40%, which is 40 times that of olive oil. In 2011, the Ministry of Health of the People's Republic of China issued Announcement No. 9 to approve peony seed oil as a new resource food. At present, peony for oil has been planted in large areas in Shandong, Henan, Shanxi, Gansu and other places in my country. With the expansion of planting area and the increase of planting years, oil peony is increasingly affected by soil-borne diseases, and the spread of the main soil-born...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00A01P21/00A01G22/60C12R1/07
CPCC12N1/20A01N63/22A01G22/60
Inventor 彭霞薇张宇汪允盛周金星
Owner BEIJING FORESTRY UNIVERSITY
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