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Production of chitose from fusarium solani

A technology of Fusarium solani and chitosan oligosaccharide is applied in the field of producing high-activity chitosan oligosaccharide, which can solve the problems of difficulty in obtaining, separation and purification, and high production cost, and achieves high enzymatic hydrolysis substrate concentration, high biological activity, and high production cost. The effect of low production cost

Inactive Publication Date: 2005-10-12
苏理
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AI Technical Summary

Problems solved by technology

[0003] The acid hydrolysis method and oxidative degradation method widely used at present to produce chitosan oligosaccharides are difficult to control the reaction conditions and separation conditions, and the post-treatment is cumbersome, separation and purification are difficult, and chitosan oligosaccharides with high physiological activity (polymerization degree is 6-10) The yield is low and the cost is high
Enzymatic degradation is mainly hydrolyzed by chitinase, chitosanase and lysozyme, but these enzymes are difficult to obtain, resulting in high production costs

Method used

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  • Production of chitose from fusarium solani

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] This kind utilizes Fusarium solani to produce the method for chitosan oligosaccharide, and its steps comprise:

[0026] 1), using Fusarium solani (Fusarium solani) to produce chitosan high-efficiency endonuclease through culture and fermentation:

[0027] Through rapid screening of plates and shake flask experiments, a strain of Fusarium solani that can rapidly degrade chitosan into chitooligosaccharides was screened; the strain was optimized to ferment chitooligosaccharides above 300U / L. carbohydrase activity;

[0028] Medium composition: glucose 2%, peptone 1%, yeast powder 0.1%, casein 0.1%; medium pH value 5.5-7.5, optimal pH value 6.0; culture time 20-64hr, optimal 60hr; selected The stirring speed is 250-300r / min; the ventilation rate: at 0-24hr, the ventilation ratio is 0.33:1; at 24-48hr, the ventilation ratio is 0.38:1; at the end of 48hr, the ventilation ratio is 0.44:1; and according to the fermentation If necessary, add polyether defoamer in time.

[0029...

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Abstract

A process of preparing chitosan used Fusarium Solami. Chitosan produced from Fusarium Solami is a kind of high efficient inscribed enzyme. Viscosity of chitosan solution can reduce to 90% in 2h between 37deg.C and 55deg.C. Producing field is bigger than 70% after 8h. Content of oligosaccharide below 10 is more than 90%. The process contains strain culture, ferment process, pre-process of material, filtering of enzymolysised solution, molecular weight assortment, decolour, desalting, concentrating and drying.

Description

Technical field: [0001] The invention is a technology for producing highly active chitosan oligosaccharides by utilizing Fusarium solani chitosan high-efficiency endonuclease. Background technique [0002] Chitosan oligosaccharide (Chitosan oligosaccharide), also known as chitosan oligosaccharide, scientific name is β-1,4-oligo-glucosamine, which is made of chitosan as raw material and degraded by biotechnology with good water solubility and high function. Low molecular weight (less than 4000D) product with strong action and high biological activity. The absorption rate of chitosan oligosaccharide in the human body is nearly 100%, and it is a functional medical food material that has attracted much attention in recent years. The water solubility of chitosan oligosaccharide is greater than 99%, and the absorption rate of human body is 99.88%, so it has better biological activity than chitosan. Experiments have shown that the application of chitosan oligosaccharides in dairy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00
Inventor 苏理鲍晓明兰文忠赵双枝董学前
Owner 苏理
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