Paenibacillus terrae YC16-08 and application thereof
A technology of bacillus and land, applied in the field of microorganisms, can solve problems such as environmental degradation, loss of soil fertility, and destruction of biodiversity
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[0033] 1. Materials and methods
[0034] 1.1 Isolation and storage of bacteria
[0035] In this example, rhizosphere soil samples were collected for the isolation of bacteria, and the rhizosphere soil samples were collected from facility greenhouses during the pepper growing season in Beidi Village, Yongchang County, Gansu Province. Randomly select 25 points in the sampling plot, take the root system of a healthy pepper plant and shake it gently with your hands until the soil on the root system does not fall off, collect the soil that has not fallen off on the root system, mix it and bring it back with a sealed bag, and remove it with a 0.2cm mesh sieve Pepper remains, air-dried at room temperature. Weigh 10g of soil sample into a conical flask filled with 90mL sterilized water, shake it for 10min, and make 10 -1 Concentration of soil suspension, let it stand until the soil particles settle, then draw 1mL supernatant and transfer it into a test tube filled with 9mL sterilize...
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