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Solid fermentation of trichoderma harzianum and application of products of solid fermentation of trichoderma harzianum in potato fusarium root rots

A technology of solid fermentation and solid plate, applied in the fields of application, fungicides, fungi, etc., can solve the problems that Fusarium cannot be eradicated, remains, and is easy to relapse, and achieves the effects of easy realization, stable growth, and simple cultivation conditions

Pending Publication Date: 2019-10-08
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are often residual problems when treated with chemical agents. At the same time, the Fusarium in the production field cannot be eradicated, and it is easy to recur in the next year. Therefore, a method that can carry out biological control of root rot is required for treatment, which is environmentally friendly, safe and effective, and can be used for long-term control.

Method used

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  • Solid fermentation of trichoderma harzianum and application of products of solid fermentation of trichoderma harzianum in potato fusarium root rots
  • Solid fermentation of trichoderma harzianum and application of products of solid fermentation of trichoderma harzianum in potato fusarium root rots
  • Solid fermentation of trichoderma harzianum and application of products of solid fermentation of trichoderma harzianum in potato fusarium root rots

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Embodiment 1

[0030] The present embodiment provides a kind of solid fermentation method of Trichoderma harzianum M-17, comprises the following steps:

[0031] Step 1. Strain cultivation: inoculate the Trichoderma harzianum M-17 strain on a PDA solid plate, and culture it at 25°C for 7 days under dark conditions;

[0032] Step 2, solid fermentation: the Trichoderma colony cultivated in step 1 is punched out along the edge of the colony with a puncher of Φ=10mm, and the bacterium cake is inoculated into the sterilized solid fermentation medium with a 10% inoculum size, and the bacterium cake is inoculated at 25 Cultivate statically at ℃ for 3 days, continue to culture after shaking the flask manually, culture temperature is 27℃, culture time is 10 days, and the culture medium can be finished after the hyphae are overgrown with the culture medium.

[0033] Wherein the solid fermentation medium components and sterilization conditions are: 50% of traditional Chinese medicine powder, 20% of whol...

Embodiment 2

[0036] The present embodiment provides a kind of solid fermentation method of Trichoderma harzianum M-17, comprises the following steps:

[0037] Step 1. Strain cultivation: inoculate the Trichoderma harzianum M-17 strain on a PDA solid plate, and culture it at 27°C for 7 days in the dark;

[0038] Step 2, solid fermentation: the Trichoderma colony cultivated in step 1 is punched out along the edge of the colony with a puncher of Φ=10mm, and the bacterium cake is inoculated into the sterilized solid fermentation medium with a 10% inoculum size, and the bacterium cake is inoculated at 25 Cultivate statically at ℃ for 3 days, continue to culture after shaking the flask manually, culture temperature is 28℃, culture time is 10 days, and the culture medium can be finished after the hyphae are overgrown with the culture medium.

[0039] Wherein the solid fermentation medium components and sterilization conditions are: 70% of traditional Chinese medicine powder, 10% of whole potato p...

Embodiment 3

[0042] The present embodiment provides a kind of solid fermentation method of Trichoderma harzianum M-17, comprises the following steps:

[0043] Step 1. Strain cultivation: inoculate the Trichoderma harzianum M-17 strain on a PDA solid plate, and culture at 28° C. for 5 days under dark conditions;

[0044] Step 2, solid fermentation: the Trichoderma colony cultivated in step 1 is punched out along the edge of the colony with a puncher of Φ=10mm, and the bacterium cake is inoculated into the sterilized solid fermentation medium with a 10% inoculum size, and the bacterium cake is inoculated at 25 Cultivate statically at ℃ for 3 days, and then continue to cultivate after shaking the flask manually. The culture temperature is 30 ℃, and the culture time is 7 days.

[0045] Wherein the solid fermentation medium components and sterilization conditions are: 30% of traditional Chinese medicine powder, 20% of whole potato flour, 30% of corn flour, 15% of sawdust, 5% of potato peel, and...

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Abstract

The invention relates to solid fermentation of trichoderma harzianum. The solid fermentation of the trichoderma harzianum comprises the steps that trichoderma harzianum M-17 strains are inoculated into a solid culture medium for culture at 28 DEG C for 7-10 days, and according to the formula, the culture medium is prepared from the components of Chinese medicinal powder, potato flakes, corn flour,wood chips and potato skins. The trichoderma harzianum cultured by the method has a good inhibiting effect on pathogenic bacteria of potato root rots such as fusarium equiseti, fusarium sambucinum, fusarium oxysporum, fusarium solani and fusarium acuminatum, and can be applied to the prevention and control process of the potato root rots, the occurrence of the potato root rots is reduced and prevented, the yield of potatoes is increased and the quality of the potatoes is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the solid fermentation of a strain of Trichoderma harzianum and the application of the product thereof in treating potato Fusarium root rot. Background technique [0002] Trichoderma is a kind of microorganism that widely exists in nature. Trichoderma belongs to the phylum Deuteromycota, Hyphospora, and the genus Trichoderma. Mold, Trichoderma harzianum, Trichoderma longibrachiata, etc. Among them, the mycelium of Trichoderma harzianum is slender and colorless, with separation and many branches. The conidiophores grow from the side branches of the mycelia, opposite or alternate, generally with 2 to 3 branches, and small conidia. Stem bottle-shaped or conical, conidia mostly spherical, spore wall with small warts, blue-green. On the PDA medium, the colony was initially white flocculent, and then dark green. [0003] Fusarium is a worldwide distribution of fungi, which c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCC12N1/14C12R2001/885C12N1/145A01N63/30
Inventor 王喜刚郭成瑾沈瑞清焦杨张丽荣杨波
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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