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Fusarium solani P-1 mycopremna and use in degradation of polychlorinated biphenyl thereof

A technology of Fusarium rot, polychlorinated biphenyls, applied in fungi, microorganism-based methods, restoration of polluted soil, etc., can solve the problems of secondary pollution, low efficiency and high cost

Inactive Publication Date: 2008-05-07
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional remediation technologies such as landfill and incineration are not only costly and inefficient, but also easily cause secondary pollution. Therefore, research in recent years has mainly focused on bioremediation technology
At present, the biodegradation of PCBs at home and abroad mainly depends on the anaerobic dechlorination and aerobic ring opening of bacteria or fungi. The anaerobic bacteria that have been isolated include Desulfomonile tiedjei, Desulfitobacterium, Dehalobacter restrictus, Dehalospirillum multivoans, Desulforomonaschloroethenica, Dehalococcoides etnenogenes, Enterobacter MS-1 and Enterobacteragglomerans, aerobic bacteria include Burkholderia sp., Pseudomonas sp., Sphingomonas sp., Rhodococcus sp., Microccus sp., Achromobacter sp., Norcardia sp., Alcalegenes sp., Arthrobacter sp., Acinetobacter sp., Janibacter sp., Bacillus sp. and Corynebacterium sp., fungi such as white rot fungi, but no fungus Fusarium solani has been found to degrade PCBs

Method used

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  • Fusarium solani P-1 mycopremna and use in degradation of polychlorinated biphenyl thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Screening of Fusarium solani P-1

[0019] Will (NH 4 ) 6 Mo 7 o 24 4H 2 O 5.28g, ZnSO 4 ·7H 2 O 29.87g, CuSO 4 1.28g, H 3 BO 3 4.0g, MnSO 4 ·H 2 O 2.805g, CoCl 2 ·6H 2 O 4.0g was made into 1L solution, and sterilized at 115°C for 20 minutes to obtain a trace salt solution. Will KH 2 PO 4 2.93g, K 2 HPO 4 ·3H 2 O5.87g, MgSO 4 0.3g, FeSO 4 ·7H 2 O 0.01g, NaCl 2.0g, (NH 4 ) 2 SO 4 5.0g, CaSO 4 ·H 2 O 0.44g, mixed with trace salt solution 2mL, supplemented with distilled water to 1000mL, pH 7.0, sterilized at 115°C for 20 minutes to obtain basic MM medium. Dissolve 2,2',4,4',6,6'-hexachlorobiphenyl in acetone, then add it to the basic MM medium, and sterilize at 115°C for 20 minutes to obtain a medium with a final concentration of 100 μg / mL p.

[0020] Take 5 g of rhizosphere soil from the mangrove rhizosphere soil of the semi-field mangrove wetland simulation system artificially polluted with PCBs for one year, dilute it 10 times...

Embodiment 2

[0022] The slant of Fusarium solani P-1 obtained in Example 1 was inoculated into tiger red agar medium, and cultured statically for 60 hours at 27°C, and the obtained bacterial strain was inoculated in 150 mL of medium P with an inoculation loop under aseptic conditions. Under the condition of 27°C, shake culture for 60 hours, collect the bacteria, wash 3 times with 0.05mol / L PBS (pH7.5), then resuspend and dilute to OD 600(600nm broad density value) is 1.0, inoculate 1% into a serum bottle containing 5mL basal MM medium and Aroclor1254 content of 50μg / mL, cover with a rubber stopper, and culture on a shaker at 30°C 200r / min for 10d. Another inoculation of the same concentration of bacterial solution in a boiling water bath for 30 minutes was used as a control.

[0023] Add 4 times the volume of n-hexane to the serum bottle, cover with a rubber stopper whose inner surface is polytetrafluoroethylene, seal it with an aluminum cap, put it into a constant temperature oscillator f...

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Abstract

The invention relates to a sort of new bacterial strain of Fusarium solani, namely P-1 CCTCC No: M207128, and also relates to the application of the Fusarium solani P-1 in degrading polychlorobiphenyl. The Fusarium solani P-1 can degrade polychlorobiphenyl effectively, in particular to aerobic condition, under which the Fusarium solani P-1 can degrade polychlorobiphenyl replaced by perchloricbiphenyl, and the average degrading rate of typical perchloricbiphenyl Aroclor 1245 can reach to 64.15 percent and the degrading rate of polychlorobiphenyl replaced by chlordane-lorobiphenyl of Aroclor 1245 can reach to 70.30 to 100 percent.

Description

technical field [0001] The invention relates to a new strain of Fusarium solani, specifically Fusarium solani (Fusarium solani) P-1 CCTCCNo: M 207128 and its application in degrading polychlorinated biphenyls. Background technique [0002] Polychlorinated biphenyls are widely distributed in polluted soil, water bodies and sediments. They have environmental persistence, bioaccumulation and potential carcinogenicity. They can withstand high temperatures of 350 ° C and are resistant to oxidants, acids and alkalis. Sex, it is difficult to be degraded. Traditional remediation technologies such as landfill and incineration are not only costly and inefficient, but also easily cause secondary pollution. Therefore, research in recent years has mainly focused on bioremediation technologies. At present, the biodegradation of PCBs at home and abroad mainly depends on the anaerobic dechlorination and aerobic ring opening of bacteria or fungi. The anaerobic bacteria that have been isolat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34B09C1/10C12R1/645
Inventor 陈桂珠孙红斌刘亚云
Owner SUN YAT SEN UNIV
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